An ATPase domain common to prokaryotic cell cycle proteins, sugar
... t, tiny (GSAT); s, small (GSATNDVCP); p, tiny plus polar (GSATNDQEKHR); x, any amino acid. Note that the groups overlap and that their names are merely mnemonic. The five-motif pattern used for the search cannot be fully represented in this simple form. The motifs are all located in the conserved su ...
... t, tiny (GSAT); s, small (GSATNDVCP); p, tiny plus polar (GSATNDQEKHR); x, any amino acid. Note that the groups overlap and that their names are merely mnemonic. The five-motif pattern used for the search cannot be fully represented in this simple form. The motifs are all located in the conserved su ...
The Arabidopsis ABHD11 Mutant Accumulates
... Figure 6. In vitro acylhydrolase activity of recombinant AtABHD11. A, The phospholipase assay was conducted with total lipids extracted from the yeast cells as a substrate (2.09 3 105 dpm/tube) with 1 mg of the recombinant, purified AtABHD11 as the enzyme source. The eluted fraction of vector backgr ...
... Figure 6. In vitro acylhydrolase activity of recombinant AtABHD11. A, The phospholipase assay was conducted with total lipids extracted from the yeast cells as a substrate (2.09 3 105 dpm/tube) with 1 mg of the recombinant, purified AtABHD11 as the enzyme source. The eluted fraction of vector backgr ...
Mineral Catalysis and Prebiotic Synthesis: Montmorillonite
... vast deposits of montmorillonite clays up to 16 m thick. The simple crystal structure of clays consists of layers of corner-linked SiO4 tetrahedra bound to layers of edgelinked AlO6 octahedra (FIG. 1). These sheets bind to each other like “a deck of cards” by van der Waals forces and interlayer cati ...
... vast deposits of montmorillonite clays up to 16 m thick. The simple crystal structure of clays consists of layers of corner-linked SiO4 tetrahedra bound to layers of edgelinked AlO6 octahedra (FIG. 1). These sheets bind to each other like “a deck of cards” by van der Waals forces and interlayer cati ...
The hepatitis C virus Core protein is a potent nucleic acid chaperone
... Core protein. Here we report that Core protein chaperones the annealing of complementary DNA and RNA sequences and the formation of the most stable duplex by strand exchange. These results show that the HCV Core is a nucleic acid chaperone similar to retroviral NC proteins. We also ®nd that the Core ...
... Core protein. Here we report that Core protein chaperones the annealing of complementary DNA and RNA sequences and the formation of the most stable duplex by strand exchange. These results show that the HCV Core is a nucleic acid chaperone similar to retroviral NC proteins. We also ®nd that the Core ...
Network Entropy and the Cancer Cell
... Therapeutic applications • A great problem is that important cancer-related genes are not directly druggable. • In these cases it may be possible to use differential flux entropy to identify neighboring viable drug targets that also exhibit significant reductions in flux entropy. • This computation ...
... Therapeutic applications • A great problem is that important cancer-related genes are not directly druggable. • In these cases it may be possible to use differential flux entropy to identify neighboring viable drug targets that also exhibit significant reductions in flux entropy. • This computation ...
Lecture-Mic 623-Plasmids-Corynebacterium - Home
... appeared to be organized in a single operon. ...
... appeared to be organized in a single operon. ...
Domains of the Adenovirus E1A Protein Required for
... Received January 16, 1991; revised version accepted May 9, 1991. ...
... Received January 16, 1991; revised version accepted May 9, 1991. ...
PDF - FEMS Microbiology Letters
... The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 58, and cloned into pIN-III(lppp-5)A3. The resulting plasmid p ...
... The gene coding for this putative decarboxylase was expressed in Escherichia coli in order to assign an unequivocal function. The gene was PCR amplified from Ent. faecium RM58 using Pfu DNA polymerase and the two synthetic primers 57 and 58, and cloned into pIN-III(lppp-5)A3. The resulting plasmid p ...
Diverse roles of the Mediator complex in plants
... and CDK8 all show developmental phenotypes due to altered cell differentiation (Wang and Chen, 2004; Gillmore et al., 2010; Ito et al., 2010). The med12 and med13 mutants are affected in the transition from globular to heart stage embryos due to a delay in the expression of KANADI1 and KANADI2 trans ...
... and CDK8 all show developmental phenotypes due to altered cell differentiation (Wang and Chen, 2004; Gillmore et al., 2010; Ito et al., 2010). The med12 and med13 mutants are affected in the transition from globular to heart stage embryos due to a delay in the expression of KANADI1 and KANADI2 trans ...
Journal of Biotechnology
... prosthetic group to which the adenylated amino acid substrate is transferred and bound as thioester. A 4 -phosphopantetheinetransferase (PPTase) converts the apo-form of the PCP into its holo-form by loading the Ppan-cofactor to an active serine. Sfp is a prototype of PPTases with wide substrate to ...
... prosthetic group to which the adenylated amino acid substrate is transferred and bound as thioester. A 4 -phosphopantetheinetransferase (PPTase) converts the apo-form of the PCP into its holo-form by loading the Ppan-cofactor to an active serine. Sfp is a prototype of PPTases with wide substrate to ...
Chapter 30: Protein Synthesis
... • 30S subunit is 930 kD with 21 different proteins and a 16S rRNA • 50S subunit is 1590 kD with 31 different proteins and two rRNAs: 23S rRNA and 5S rRNA • Ribosomes are roughly 2/3 RNA by mass • 20,000 ribosomes in an E. coli cell, 20% of ...
... • 30S subunit is 930 kD with 21 different proteins and a 16S rRNA • 50S subunit is 1590 kD with 31 different proteins and two rRNAs: 23S rRNA and 5S rRNA • Ribosomes are roughly 2/3 RNA by mass • 20,000 ribosomes in an E. coli cell, 20% of ...
FOG1 recruits the NuRD repressor complex to mediate
... than that observed with GATA-1-ER (Figure 1C), suggesting that FOG-1 is not required for GATA-1 occupancy at these sites. Similar results were obtained with a second independently derived GATA-1(V205M)-ER-expressing cell line (data not shown). As an additional control, GATA-1 occupancy was determine ...
... than that observed with GATA-1-ER (Figure 1C), suggesting that FOG-1 is not required for GATA-1 occupancy at these sites. Similar results were obtained with a second independently derived GATA-1(V205M)-ER-expressing cell line (data not shown). As an additional control, GATA-1 occupancy was determine ...
Current Opinion in Plant Biology
... have been studied in most detail. All of these bacteria form a host-specific symbiosis with leguminous plants. The symbiosis is initiated by the formation of root or stem nodules in response to the presence of the bacterium. Lipooligosacharide signal molecules that are secreted by the bacterium play ...
... have been studied in most detail. All of these bacteria form a host-specific symbiosis with leguminous plants. The symbiosis is initiated by the formation of root or stem nodules in response to the presence of the bacterium. Lipooligosacharide signal molecules that are secreted by the bacterium play ...
shaping our food
... that many traits depend on many genes, so called quantitative traits, was understood and statistical models were developed to account for such traits in livestock breeding. As with evolution, breeding is dependent on genetic variation and the recombination of genes. However, genetic variation can be ...
... that many traits depend on many genes, so called quantitative traits, was understood and statistical models were developed to account for such traits in livestock breeding. As with evolution, breeding is dependent on genetic variation and the recombination of genes. However, genetic variation can be ...
Section Title – One Line Preferred, Two Line Maximum
... copy of the RNA target. Reverse transcription primer: a single short DNA molecule that acts as starting points for the reverse transcriptase, since reverse transcriptases cannot initiate DNA synthesis without a primer. Deoxynucleotide triphosphates (dNTPs): the building blocks for the newly synthesi ...
... copy of the RNA target. Reverse transcription primer: a single short DNA molecule that acts as starting points for the reverse transcriptase, since reverse transcriptases cannot initiate DNA synthesis without a primer. Deoxynucleotide triphosphates (dNTPs): the building blocks for the newly synthesi ...
The polymerase chain reaction
... before adding the polymerase. Specialized enzyme systems have been developed that inhibit the polymerase's activity at ambient temperature, either by the binding of an antibody or by the presence of covalently bound inhibitors that dissociate only after a high-temperature activation step. Hot-start/ ...
... before adding the polymerase. Specialized enzyme systems have been developed that inhibit the polymerase's activity at ambient temperature, either by the binding of an antibody or by the presence of covalently bound inhibitors that dissociate only after a high-temperature activation step. Hot-start/ ...
Analysis of Transcription Activation Distance as a Polygenic Trait in
... regulation of gene expression. Work over the past few decades has provided vast insight into the proteins and mechanisms governing this step. In eukaryotes, most fundamental aspects of transcription initiation are conserved from yeast to humans. This conservation, as well as the ability to perform h ...
... regulation of gene expression. Work over the past few decades has provided vast insight into the proteins and mechanisms governing this step. In eukaryotes, most fundamental aspects of transcription initiation are conserved from yeast to humans. This conservation, as well as the ability to perform h ...
Mutually exclusive splicing
... When 5’ splice site is encountered in the newly synthesized RNA, the factors that recognize that site(5’ splice site), transferred from C-terminal tail of RNA polymerase II onto RNA. Once in place, the 5’ splice site components are poised to interact with those other factors that binds to the next 3 ...
... When 5’ splice site is encountered in the newly synthesized RNA, the factors that recognize that site(5’ splice site), transferred from C-terminal tail of RNA polymerase II onto RNA. Once in place, the 5’ splice site components are poised to interact with those other factors that binds to the next 3 ...
Biochemistry - Stryer - Science and Technology
... ince its emergence in the 1970s, recombinant DNA technology has revolutionized biochemistry. The genetic endowment of organisms can now be precisely changed in designed ways. Recombinant DNA technology is the fruit of several decades of basic research on DNA, RNA, and viruses. It depends, first, on ...
... ince its emergence in the 1970s, recombinant DNA technology has revolutionized biochemistry. The genetic endowment of organisms can now be precisely changed in designed ways. Recombinant DNA technology is the fruit of several decades of basic research on DNA, RNA, and viruses. It depends, first, on ...
NEHRU ARTS AND SCIENCE COLLEGE DEPARTMENT OF
... the tiny intact embryos, so often ovaries with young embryos, or entire fertilized ovules will be used. Factors to consider Embryos are manually excised and placed immediately onto a culture media that provides the proper nutrients to support survival and growth (Miyajima 2006). While the disinfesta ...
... the tiny intact embryos, so often ovaries with young embryos, or entire fertilized ovules will be used. Factors to consider Embryos are manually excised and placed immediately onto a culture media that provides the proper nutrients to support survival and growth (Miyajima 2006). While the disinfesta ...
Regulation of Galactoside Transport by the PTS
... Among four proteins (LacY, MelB, MalK, and GlpK) that interact with IIAGlc, MelB is considered to be a little different one, since some of the conserved residues in REGION II are absent. In fact, our recent experiments demonstrated that deletion of Arg-215 to Leu-220, which corresponds to the latter ...
... Among four proteins (LacY, MelB, MalK, and GlpK) that interact with IIAGlc, MelB is considered to be a little different one, since some of the conserved residues in REGION II are absent. In fact, our recent experiments demonstrated that deletion of Arg-215 to Leu-220, which corresponds to the latter ...
7.06 Cell Biology EXAM #3
... For each of the following engineered proteins, predict which location in the cell they would be targeted to. Assume that each targeting sequence is at the site along the protein (N terminus, C terminus, internal) where it would normally be found. Also assume that the only targeting sequences possess ...
... For each of the following engineered proteins, predict which location in the cell they would be targeted to. Assume that each targeting sequence is at the site along the protein (N terminus, C terminus, internal) where it would normally be found. Also assume that the only targeting sequences possess ...
Predictable Alteration of Sequence Recognition by RNA
... OTP82, and using the modified proteins to attempt to complement the respective mutants, we demonstrate that we can predictably alter the specificity of these factors in vivo. ...
... OTP82, and using the modified proteins to attempt to complement the respective mutants, we demonstrate that we can predictably alter the specificity of these factors in vivo. ...