Pol /Primase, Pol ε Pol ε α MIT Department of Biology 7.28, Spring
... Pol ε and Pol δ can load independently of one another and Pol α/Primase. Pol α /Primase only loads in the last experiment when both Pol ε and Pol δ are present, and cannot load in the first two lanes when only one or the other is present. The simplest hypothesis is that Pol ε and Pol δ both can load ...
... Pol ε and Pol δ can load independently of one another and Pol α/Primase. Pol α /Primase only loads in the last experiment when both Pol ε and Pol δ are present, and cannot load in the first two lanes when only one or the other is present. The simplest hypothesis is that Pol ε and Pol δ both can load ...
Amino Acid Sequence Homology of Coat Proteins as a Basis for
... The potyvirus group is the largest and economically the most important of the 28 plant virus groups and families (Matthews, 1982; Brown, 1986). Some members of the group are very successful pathogens and in 1974 were reported to infect 1112 species of 369 genera in 53 plant families (Edwardson, 1974 ...
... The potyvirus group is the largest and economically the most important of the 28 plant virus groups and families (Matthews, 1982; Brown, 1986). Some members of the group are very successful pathogens and in 1974 were reported to infect 1112 species of 369 genera in 53 plant families (Edwardson, 1974 ...
PALI—a database of Phylogeny and ALIgnment of homologous
... 3-D structures of homologous proteins with clear sequence similarity have highly similar structures and often have similar biological roles in the living systems (for examples see 4–6). Variation in the amino acid sequences of homologous proteins in a family is constrained by the high similarity in ...
... 3-D structures of homologous proteins with clear sequence similarity have highly similar structures and often have similar biological roles in the living systems (for examples see 4–6). Variation in the amino acid sequences of homologous proteins in a family is constrained by the high similarity in ...
Iron-sulfur proteins and the role of iron in the gene expression of
... 2. FeS clusters and FeS proteins Proteins that contain iron-sulfur (FeS) clusters as a prosthetic group are widely utilised in all living organisms for a variety of cellular processes. Inorganic FeS metal compounds were already present in hydrothermal vents in the anaerobic atmosphere of the ancien ...
... 2. FeS clusters and FeS proteins Proteins that contain iron-sulfur (FeS) clusters as a prosthetic group are widely utilised in all living organisms for a variety of cellular processes. Inorganic FeS metal compounds were already present in hydrothermal vents in the anaerobic atmosphere of the ancien ...
Preparing and Analyzing Expressed Sequence Tags (ESTs) Library
... over 80% similarity to known sequences of other organisms in NCBI were classified according to GO terms such as molecular function, biological process, cellular components, protein class, and pathway with PANTHER database (Figures 5 and 6). 154 and 268 ESTs of these total ESTs were prenatal and post ...
... over 80% similarity to known sequences of other organisms in NCBI were classified according to GO terms such as molecular function, biological process, cellular components, protein class, and pathway with PANTHER database (Figures 5 and 6). 154 and 268 ESTs of these total ESTs were prenatal and post ...
activator protein
... Repressible Genes • enzymes that function in biosynthetic pathways are products of repressible genes • generally these enzymes are always present unless the end product in the biosynthetic pathway is available ...
... Repressible Genes • enzymes that function in biosynthetic pathways are products of repressible genes • generally these enzymes are always present unless the end product in the biosynthetic pathway is available ...
Global MicroRNA Amplification Kit
... The study of noncoding RNAs, especially noncoding micro RNAs (miRNA), has gained increasing attention in recent years. Micro RNAs are 19-24 nucleotide long single stranded RNAs that regulate the expression of target genes by interacting with complementary sites in the 3’ UTR of the target mRNAs and ...
... The study of noncoding RNAs, especially noncoding micro RNAs (miRNA), has gained increasing attention in recent years. Micro RNAs are 19-24 nucleotide long single stranded RNAs that regulate the expression of target genes by interacting with complementary sites in the 3’ UTR of the target mRNAs and ...
Article, Discoveries Variation in mutational robustness between
... used fitness assays. We show that the fitness effects of amino acid substitutions can be predicted based on evolutionary conservation, but those weakly deleterious mutations are less reliably detected. This suggests that large-effect mutations and the fraction of highly deleterious mutations can be ...
... used fitness assays. We show that the fitness effects of amino acid substitutions can be predicted based on evolutionary conservation, but those weakly deleterious mutations are less reliably detected. This suggests that large-effect mutations and the fraction of highly deleterious mutations can be ...
Is β-pleated sheet the molecular conformation which dictates
... prediction scheme (Hamodrakas, S.J., 1988. A protein secondary structure prediction scheme for the IBM PC and compatibles. CABIOS 4, 473–477) that combines six different algorithms predicting α-helix, β-strands and β-turn/loops/coil has been used to predict the secondary structure of chorion protein ...
... prediction scheme (Hamodrakas, S.J., 1988. A protein secondary structure prediction scheme for the IBM PC and compatibles. CABIOS 4, 473–477) that combines six different algorithms predicting α-helix, β-strands and β-turn/loops/coil has been used to predict the secondary structure of chorion protein ...
Identification of a family of BspA like surface proteins of Entamoeba
... with the concept that these genes could have come to E. histolytica via lateral gene transfer from prokaryotes [8]. With the completion of the E. histolytica genome project, we used the InterProScan software (http://www.ebi.ac.uk/ InterProScan) to screen for additional E. histolytica sequences that ...
... with the concept that these genes could have come to E. histolytica via lateral gene transfer from prokaryotes [8]. With the completion of the E. histolytica genome project, we used the InterProScan software (http://www.ebi.ac.uk/ InterProScan) to screen for additional E. histolytica sequences that ...
Infrared spectroscopic studies: from small molecules to large.
... control its binding to the target protein. With the help of ATR-FTIR spectroscopy and a specially designed dialysis setup, a general method was developed to detect ligand binding events by observing IR absorbance changes in the hydration shell around the molecules. ATR-FTIR spectroscopy was also use ...
... control its binding to the target protein. With the help of ATR-FTIR spectroscopy and a specially designed dialysis setup, a general method was developed to detect ligand binding events by observing IR absorbance changes in the hydration shell around the molecules. ATR-FTIR spectroscopy was also use ...
The Cytoskeleton
... GTPases: cdc42: its activation triggers actin polymerization and bundling at ...
... GTPases: cdc42: its activation triggers actin polymerization and bundling at ...
PowerPoint (click here)
... At least some of the residues that are important for ubiquitin ligase activity (452, 455, and 457) are conserved in the mystery protein. The one residue that we checked that was not conserved (374) seems to be less important for activity. These results are consistent with the possibility that the my ...
... At least some of the residues that are important for ubiquitin ligase activity (452, 455, and 457) are conserved in the mystery protein. The one residue that we checked that was not conserved (374) seems to be less important for activity. These results are consistent with the possibility that the my ...
Bioinformatics in Brief This week: DB for structures Structure
... Many unexpected links: • Histon and heat-shock protein ...
... Many unexpected links: • Histon and heat-shock protein ...
lecture4
... tRNA is present for each of the 20 amino acids used in protein synthesis. (Some amino acids employ the services of two or three different tRNAs, so most cells contain as many as 32 different kinds of tRNA.) The amino acid is attached to the appropriate tRNA by an activating enzyme (one of 20 aminoac ...
... tRNA is present for each of the 20 amino acids used in protein synthesis. (Some amino acids employ the services of two or three different tRNAs, so most cells contain as many as 32 different kinds of tRNA.) The amino acid is attached to the appropriate tRNA by an activating enzyme (one of 20 aminoac ...
Fundamentals of Biochemistry. Life at the Molecular Level. 3rd Edition Brochure
... Brochure More information from http://www.researchandmarkets.com/reports/2242601/ ...
... Brochure More information from http://www.researchandmarkets.com/reports/2242601/ ...
Document
... manipulation; hybridoma technology and its applications; cell fusion methods; gene mapping; vaccine production; gene therapy, targeting, silencing and knockout. Selectable markers like pSV and pRSV plasmids -reporter genes. Application of animal cell culture - Engineered cell culture as source of va ...
... manipulation; hybridoma technology and its applications; cell fusion methods; gene mapping; vaccine production; gene therapy, targeting, silencing and knockout. Selectable markers like pSV and pRSV plasmids -reporter genes. Application of animal cell culture - Engineered cell culture as source of va ...
PHYS 4xx Intro 2 1 PHYS 4xx Intro 2
... A protein chain of high molecular mass will be built up as this reaction occurs repeatedly; for example, the two inequivalent spectrin proteins in the human erythrocyte have molecular masses of ~220 000 and ~230 000 Da. Amino acids appear in a protein with varying relative abundance, and some, such ...
... A protein chain of high molecular mass will be built up as this reaction occurs repeatedly; for example, the two inequivalent spectrin proteins in the human erythrocyte have molecular masses of ~220 000 and ~230 000 Da. Amino acids appear in a protein with varying relative abundance, and some, such ...
ppt - Chair of Computational Biology
... The main function of miRNAs is to down-regulate gene expression of their target mRNAs. miRNAs typically have incomplete base pairing to a target and inhibit the translation of many different mRNAs with similar sequences. In contrast, siRNAs typically base-pair perfectly and induce mRNA cleavage only ...
... The main function of miRNAs is to down-regulate gene expression of their target mRNAs. miRNAs typically have incomplete base pairing to a target and inhibit the translation of many different mRNAs with similar sequences. In contrast, siRNAs typically base-pair perfectly and induce mRNA cleavage only ...
biochem ch 45 [9-2
... o Factor XIII cross-links strands of of polymerized fibrin monomers to form secondary hemostatic plug o Ca2+ and γ-carboxyglutamate residues in proteins (formed by vitamin K-dependent process in liver) attach factor complexes to phospholipids exposed on platelet membranes, causing thrombus formation ...
... o Factor XIII cross-links strands of of polymerized fibrin monomers to form secondary hemostatic plug o Ca2+ and γ-carboxyglutamate residues in proteins (formed by vitamin K-dependent process in liver) attach factor complexes to phospholipids exposed on platelet membranes, causing thrombus formation ...
Molecule of the Month: AgrA DNA Binding Domain AgrA is the
... because other nucleotides in the binding site are quite important for binding (and conserved among other LytTR binding site) indicating that they may play an important role in indirect binding or in allowing for proper positing of the DNA binding site3. Isothermal titration calorimetry was performed ...
... because other nucleotides in the binding site are quite important for binding (and conserved among other LytTR binding site) indicating that they may play an important role in indirect binding or in allowing for proper positing of the DNA binding site3. Isothermal titration calorimetry was performed ...
lecture4
... How does a particular sequence of nucleotides specify a particular sequence of amino acids? The answer: by means of transfer RNA molecules, each specific for one amino acid and for a particular triplet of nucleotides in messenger RNA (mRNA) called a codon. The family of tRNA molecules enables the co ...
... How does a particular sequence of nucleotides specify a particular sequence of amino acids? The answer: by means of transfer RNA molecules, each specific for one amino acid and for a particular triplet of nucleotides in messenger RNA (mRNA) called a codon. The family of tRNA molecules enables the co ...
Activity of ribosomes and tmRNA of Streptomyces aureofaciens
... with coordinated expression of multiple genes. Energy expenses for sRNA synthesis are several times lower than for synthesis of regulatory proteins. This type of regulation may represent ancestral form of control of gene functions probably before appearance of regulatory proteins. Streptomycetes are ...
... with coordinated expression of multiple genes. Energy expenses for sRNA synthesis are several times lower than for synthesis of regulatory proteins. This type of regulation may represent ancestral form of control of gene functions probably before appearance of regulatory proteins. Streptomycetes are ...
Highly Efficient Micro RNA Enrichment
... to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of ...
... to 40 nucleotides, and the majority of the miRNA is composed of approximately 22 nucleotides. Most of the commercially available miRNA extraction protocols co-purify the miRNA and total RNA. Therefore, the extracted samples still contain ribosomal RNA and messenger RNA with only a low percentage of ...
Origin and Evolution of a New Gene Descended From alcohol
... two Adh isozymes are present (BATTERHAM et al. 1984). Third, in the two species examined, D. hydei and D. mulleri, the putative Adh pseudogene is transcribed only in pupae and/or adults (FISCHERand MANIATIS 1985; SULLIVANet al. 1994), an expression pattern different from that of other Drosophila Adh ...
... two Adh isozymes are present (BATTERHAM et al. 1984). Third, in the two species examined, D. hydei and D. mulleri, the putative Adh pseudogene is transcribed only in pupae and/or adults (FISCHERand MANIATIS 1985; SULLIVANet al. 1994), an expression pattern different from that of other Drosophila Adh ...