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DNA Replication/Transcription/RNA Splicing
DNA Replication/Transcription/RNA Splicing

... Sites of Ongoing Transcription The intranuclear position of many genes has been correlated with their activity state, suggesting that migration to functional subcompartments may influence gene expression. Indeed, nascent RNA production and RNA polymerase II seem to be localized into discrete foci or ...
notes pdf - Auburn University
notes pdf - Auburn University

... H. for an average-sized polypeptide chain (~300-400 amino acids long) translation takes less than a minute I. ...
Gel electrophoresis - University of California, Santa Barbara
Gel electrophoresis - University of California, Santa Barbara

... translated into amino acid sequences • The “words” of the DNA “language” are triplets of bases called codons – 3 bases or nucleotides make one codon – Each codon specifies an amino acid – The codons in a gene specify the amino acid sequence of a polypeptide ...
Origins of Sugars in the Prebiotic World
Origins of Sugars in the Prebiotic World

... • If the nucleophile is the 3’-OH group of another NTP, then a nucleic acid is generated: polymer of nucleotides – Oligomers (“oligos”)  short length (DNA/RNA polymers of long ...
Lecture 2a – Origin of Life and the transition from the RNA world to
Lecture 2a – Origin of Life and the transition from the RNA world to

... inefficient (for example, because the new and old copies have to completely separate before further selfreplication can occur), and also it is essentially random (because the initial copy has to form in the absence of a template). So we think that “in the beginning” there must have been a catalyst f ...
notes 12B
notes 12B

... 2. DNA is a template for _______________ formation during _______________. 3. Transcription is the first step in gene _______________; it is the process whereby a DNA strand serves as a template for the formation of _______________. 4. During translation, an mRNA _______________ vdirects the sequenc ...
Biology and computers - Cal State LA
Biology and computers - Cal State LA

... Copy the FASTA output for both species into a single text file. Make sure the header is separate from the sequence. Clink on the link, find the FASTA format and copy into the same file ...
From Genes to Proteins What do genes code for?
From Genes to Proteins What do genes code for?

... • Some tRNA may bind with more than one codon (Supports redundancy) • “Wobble” hypothesis is that anticodon with U in third position can bind to A or G ...
PowerPoint 演示文稿
PowerPoint 演示文稿

... in a complex together with cellular RNA-binding factors. • This protein-RNA complex may result in the activation of the kinase activity of TFIIH. • This leads to phosphorylation of the CTD of RNA Pol II, Section N: Regulation of transcrip. in Euk. ...
Positive Strand RNA Viruses
Positive Strand RNA Viruses

... It seems that binding to the receptor alters capsid structure in some way, a channel forms across the cell membrane and the RNA is released into cytoplasm. The mRNA is now available for translation. ...
1. Ribonucleic acid is not normally associated with the (1) cytoplasm
1. Ribonucleic acid is not normally associated with the (1) cytoplasm

... 10. Which nucleic acid carries instructions from the nucleus to the cytoplasm? (3) Transfer RNA, only (1) DNA, only (4) DNA, messenger RNA, and transfer RNA (2) Messenger RNA, only 11. Which of the following nucleic acids are composed of nucleotides? (3) Transfer RNA, only (1) DNA, only (2) Messenge ...
Protein Synthesis
Protein Synthesis

... • Amino Acids are the building blocks for proteins • Since there are 4 nucleotides, when three are grouped together, there are 64 possible triplet combinations (43 = 64) • However, there are only 20 amino acids so some amino acids have more than one codon (ex. GGA, GGC, and GGG all code for glycine) ...
C H E M I S T R Y
C H E M I S T R Y

... (lactose) binds to the repressor protein, it changes shape and falls off of the operator region. Now RNA polymerase can pass and transcribe the genes into mRNA. ...
Exam 4
Exam 4

... A) Prokaryotic translation starts at AUG, which codes for methionine B) Prokaryotic mRNA receives a 5’ cap before translation C) In prokaryotes, transcription and translation of an RNA molecule can occur at the same time D) Prokaryotic DNA includes a promoter for each gene E) Prokaryotic ribosomes s ...
From DNA to Proteins
From DNA to Proteins

... • Three types of RNA are needed for protein synthesis – Messenger RNA (mRNA) carries the blueprint for protein assembly to the ribosome. – Ribosomal RNA (rRNA) combines with proteins to form ribosomes upon which polypeptides are assembled. – Transfer RNA (tRNA) brings the correct amino acid to the r ...
Unit 6B Learning Targets
Unit 6B Learning Targets

... ii. Addition of a GTP cap iii. Excision of introns c. Translation of the mRNA occurs in the cytoplasm on the ribosome. d. In prokaryotic organisms, transcription is coupled to translation of the message. Translation involves energy and many steps, including initiation, elongation, and termination. i ...
amino acids
amino acids

... RNA is single- stranded RNA contains the base uracil instead of thymine. Like thymine, uracil can form bonds with adenine ...
Topic 3.5 Transcription (9-13)
Topic 3.5 Transcription (9-13)

... cytoplasm where he enzymes, ribosomes, and amino acids are found ...
1. Overview of Gene Expression Overview of Gene Expression Chapter 10B:
1. Overview of Gene Expression Overview of Gene Expression Chapter 10B:

... 2) ribosome binds 2 tRNA-AAs, 2 codons at a time • i.e., tRNAs with anti-codons complementary to the mRNA codons ...
Slide 1
Slide 1

... Genes for tryptophan synthesis Repressed by end-product of pathway, Tryptophan Repression requires Operator sequence, Aporepressor (trpR gene product) & Corepressor (Tryptophan) - Operator is within the promoter Also controlled by attenuation in the “Leader” region of the transcript ...
1 BIOS 1300 SI SI WORKSHEET 8 (Chapter 3 Cont.) SI Leader
1 BIOS 1300 SI SI WORKSHEET 8 (Chapter 3 Cont.) SI Leader

... growing mRNA chain 3. Termination - In prokaryotes, transcription ends once a ___________________ sequence is transcribed - In eukaryotes, transcription ends 10-35 nucleotides after a _________________________________ is transcribed II. RNA processing: modifications to an mRNA transcript that occur ...
Protein Synthesis
Protein Synthesis

... • mRNA picks up the code from the DNA template strand and takes it from the nucleus to ribosomes in the cytoplasm • Main goal: make a copy of the code and get it out of the nucleus! Question: Why can’t DNA leave the nucleus? ...
DNA,Rep,RNA,Trans pp
DNA,Rep,RNA,Trans pp

... 2. Why must the copy be exact? to prevent mutations ...
DNA - Moodle
DNA - Moodle

... termination • RNA polymerase reaches termination region of the gene, which marks the end of the coding sequence • terminates transcription by releasing both DNA and RNA • maximum 3 marks from each of initiation, elongation, termination ...
Temporal control of Transcription in phage SPO
Temporal control of Transcription in phage SPO

... Cro vs cI •  Cro and cI are DNA binding proteins that bind to the operator sequences OR and OL •  There are three binding sites for each protein in the operator •  Cro and cI bind with opposite affinities to these three sites •  cI binds first to site 1, then 2 and at high concentrations site 3 •  ...
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RNA polymerase II holoenzyme

RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding genes in living cells. It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins.
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