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DNA, RNA and Protein
DNA, RNA and Protein

... of the new double-helical molecules? ...
Central Dogma of Molecular Biology
Central Dogma of Molecular Biology

... control your characteristics? DNA contains instructions for all the proteins your body makes. Proteins, in turn, determine the structure and function of all your cells. What determines a protein’s structure? It begins with the sequence of amino acids that make up the protein. Instructions for making ...
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M-MuLV Reverse Transcriptase, RNase H minus

... M-MuLV Reverse transcriptase is purified from an E.coli strain harbouring a plasmid that directs the synthesis of a modified form of Moloney Murine Leukemia virus (M-MuLV) reverse transcriptase. M-MuLV reverse transcriptase is a RNA or DNA directed DNA polymerase. The enzyme can synthesize a complem ...
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... Post-transcriptional Modifcation non-coding introns removed from mRNA  Coding exons spliced together to make the mRNA that will be used in translation  multiple splicing patterns for each “pre-mRNA”  1 gene  multiple mRNA/proteins ...
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... Single stranded bases within a stem are called a bulge of bulge loop if the single stranded bases are on only one side of the stem. If single stranded bases interrupt both sides of a stem, they are called an internal ...
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Assignment 5 (Perl Project 2)

... A DNA string, which we will also call a DNA strand, is a nite sequence of the lowercase letters a, c, g, and t in any order. For example, acgtacccggttt is a small DNA strand. The four letters stand for the four nucleotides : adenine, cytosine, guanine, and thymine. Nucleotides, which are the molecu ...
Molecular Biology
Molecular Biology

... • RNA polymerase II (Pol II; also known as RNAP II). It transcribes protein-encoding genes into mRNA (and also the snRNA genes). • RNA polymerase III (Pol III). It transcribes the 5S rRNA genes and all the tRNA genes. ...
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... regulation; Binding site of tobacco TGA1a (a member of the bZip family of TFs; Abiotic and biotic stress differentially stimulate "as-1 element" activity "C-box" according to the nomenclature of ACGT elements by Foster et al. (1994); One of ACGT elements; Factors groups 2 and 3 have affinity for C-b ...
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SBI-4U1 Exam Review

... You are allowed to bring a rough draft into the exam. If you do so, it must be submitted with the exam. Your answer must be in essay format with complete sentences and paragraphs. Content, organization, grammar and spelling all count. 1. Discuss the reasons why the development of: proteins and nucle ...
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... mRNA: ________________________ 2. DNA: TAC GGG ACA GGT ATT mRNA: ________________________ 3. DNA: TAC CCT ATG CCA ATC mRNA: ________________________ ...
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ppt - Duke Computer Science
ppt - Duke Computer Science

... Usually these are proteins . . . . But they can also be RNA or DNA. That is, single stranded RNA or DNA molecules can and will fold up into secondary and tertiary structures depending on their sequence. DNA can be synthesized as very large numbers of different (random sequences) Aptamers can be sele ...
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... Splicing of mRNA precursors. A representative precursor mRNA is depicted. Exon 1 and Exon 2 indicate two exons separated by an intervening sequence (or intron) with consensus 5, 3, and branch sites. The fate of the phosphates at the 5- and 3’-splice sties can be followed by tracing the fate of t ...
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NZY First-Strand cDNA Synthesis Kit

... 6. Use the cDNA product directly in PCR or qPCR diluted in TE buffer or undiluted; or store at -20 °C until required. ...
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... 1. A repressible operon is one that is usually on; binding of a repressor to the operator shuts off transcription. 1. The trp operon is a repressible operon. trp operon ...
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ppt2 DNA Transcription and Translation

... RNA polymerase binds to the promoter site (TATA box) (start) on the DNA RNA polymerase adds RNA nucleotides complimentary to the DNA strand mRNA building is complete when the RNA polymerase reaches a Termination (stop) site on the DNA This strand of mRNA is EDITED before leaving the nucleus & carryi ...
Assignment on DNA, RNA, Transcription and Translation
Assignment on DNA, RNA, Transcription and Translation

... can’t get out. To solve this problem, copies of the DNA are made in a form called mRNA. The process of making mRNA from DNA is called transcription. After transcription, the mRNA copies leave the nucleus to be in the part of the cell outside the nucleus, otherwise known as the cytoplasm. mRNA can’t ...
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RNA polymerase II holoenzyme

RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding genes in living cells. It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins.
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