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Chapter02 Nucleotides and Nucleic Acids(核酸化学)
Chapter02 Nucleotides and Nucleic Acids(核酸化学)

... Splicing produces final mRNA without introns Ribosomal RNA Ribosomes are about 2/3 RNA, 1/3 protein rRNA serves as a scaffold for ribosomal proteins 23S rRNA in E. coli is the peptidyl transferase! Transfer RNA Small polynucleotide chains - 73 to 94 residues each Several bases usually methylated Eac ...
Quant-iT™ Assay Kits for microplate
Quant-iT™ Assay Kits for microplate

... Figure 1—Quantitation using Quant-iT™ Assay Kits. A The Quant-iT™ dsDNA Assay Kit, High Sensitivity has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the ...
Build-a-Bug - Wando High School
Build-a-Bug - Wando High School

... During this step, mRNA goes through different types of maturation including one called splicing, when the non-coding sequences are eliminated. The coding mRNA sequence can be described as a unit of three nucleotides called a codon. 2. In Table 1., transcribe your DNA strand into the “language” of mR ...
Build-a-Bug - Wando High School
Build-a-Bug - Wando High School

... During this step, mRNA goes through different types of maturation including one called splicing, when the non-coding sequences are eliminated. The coding mRNA sequence can be described as a unit of three nucleotides called a codon. 2. In Table 1., transcribe your DNA strand into the “language” of mR ...
Preparation of MyoD mRNA for the differentiation of stem cells into
Preparation of MyoD mRNA for the differentiation of stem cells into

... Several other studies revealed that RNAs can also act as a transcription factor in cell differentiation. MicroRNAs (miRNAs), which are small noncoding RNAs, can affect and guide the RNA for transcription. Furthermore, the presence of small interfering RNAs (siRNAs) can create an environment similar ...
trp
trp

... cap)? How and when is the poly-A tail added to the transcript? Where does eukaryotic RNA processing occur? 7. Understand the structure and function of promoters and terminators in transcription. Contrast intrinsic terminators and rho-dependent terminators. 8. Know the subunit structure of bacterial ...
The Genetic Code The nucleotide bases of the DNA strand
The Genetic Code The nucleotide bases of the DNA strand

... Once the tRNA has found it’s position along the mRNA it locks into a distinct place. The tRNA also carries – as a specific correlation to its nucleotide sequence - one distinct amino acid. A tRNA is therefore distinct for its three nucleotides at one end, and its proper amino acid at the other end o ...
Control of Gene Expression in Prokaryotes.
Control of Gene Expression in Prokaryotes.

... promoter is now unmasked and RNA polymerase can now bind and initiate transcription. However it won’t do this very frequently without the help of the cAMP-CAP bound to the activation site. This protein complex binding puts a 90o kink in the DNA and interacts with the alpha subunit of RNA polymerase. ...
Solution structure of the Drosha double-stranded RNA-binding domain Open Access
Solution structure of the Drosha double-stranded RNA-binding domain Open Access

... in the model. The substrates of Drosha are hairpin primiRNA with mismatched and bulged bases that would form irregular structures. Thus the substrate RNA could be bent and the protein loops could alter conformation to allow interaction. DGCR8 contains two dsRBDs, which recognize primiRNA [18-20]. In ...
Document
Document

... •Required but not a part of the RNA polymerase complex •Many different roles in gene regulation Binding Interaction Initiation Enhancing Repressing •Various structural classes (eg. zinc finger domains) •Consist of both a DNA-binding domain and an interactive domain ...
Control of Gene Expression in Prokaryotes.
Control of Gene Expression in Prokaryotes.

... promoter is now unmasked and RNA polymerase can now bind and initiate transcription. However it won’t do this very frequently without the help of the cAMP-CAP bound to the activation site. This protein complex binding puts a 90o kink in the DNA and interacts with the alpha subunit of RNA polymerase. ...
General Biology Notes CH 12: TRANSLATION A.K.A. PROTEIN
General Biology Notes CH 12: TRANSLATION A.K.A. PROTEIN

... into a sequence of amino acids that makes up proteins. ...
Eukaryotic Regulation
Eukaryotic Regulation

... Reporter assay in transgenic mouse ...
Lecture 27
Lecture 27

... convert a primary transcript into a final function RNA product •Eukaryotic pre-mRNAs are capped, polyadenylated, and spliced to yield one or more mature mRNAs before transport to the cytoplasm. These processes are coupled in the nucleus so that only properly processed mRNAs are exported to the cytop ...
Operon Info_pGLO pre lab
Operon Info_pGLO pre lab

... Repressible and Inducible Operons: Two Types of Negative Gene Regulation  A repressible operon is one that is usually on; binding of a repressor to the operator shuts off transcription  The trp operon is a repressible operon  An inducible operon is one that is usually off; a molecule called an i ...
protein synthesis worksheet
protein synthesis worksheet

... PART A. Read the following: Protein synthesis is the process used by the body to make proteins. The first step of protein synthesis is called Transcription. It occurs in the nucleus. During transcription, mRNA transcribes (copies) DNA. DNA is “unzipped” and the mRNA strand copies a strand of DNA. On ...
Solid Waste in History
Solid Waste in History

...  Asymmetric creation of a growing bud, on the mother cell.  The bud increases in size and eventually severed from the parental cell.  After division is complete, the mother cell reinitiates the process by growing another bud.  Yeast and some bacteria (Caulobacter is one example) use this form of ...
Functional dissection of the baculovirus late expression factor
Functional dissection of the baculovirus late expression factor

... The late expression factor-8 gene (lef-8) of Autographa californica M nucleopolyhedrovirus encodes the largest subunit of the virally encoded DNA-directed RNA polymerase specific for the transcription of late and very late viral genes. The sequence of lef-8 predicts a C-terminal motif of 13 amino ac ...
supporting information file s1
supporting information file s1

... CoaE. In order to aid the selection of modeling templates for the N- and C-terminal domains of the mycobacterial CoaE, the Sequence Feature Scan tool from the Swiss-Model server which helps predict the secondary structure, presence of disordered regions and helps assign domains in the target sequen ...
Knowledge Entry as the Graphical Assembly of Components
Knowledge Entry as the Graphical Assembly of Components

... Equational information (e.g., rates wrt time) Temporal relations (e.g., simultaneous) Pre/post conditions for actions Richer process models (e.g., repetitive events) Sequences (e.g., nucleotide sequences) Negative information (e.g., doesn’t happen) Locational/spatial information (e.g., shape) Ch ...
translation ppt
translation ppt

... Termination ...
Chapter 8
Chapter 8

... • The language of nucleic acids is A,C,T,G, & U’s, but the language of proteins is amino acids (remember these are the monomers of proteins) • The A,C,T,G, &U’s of a nucleic acid are in a very ...
Genome_annotation
Genome_annotation

... Transcription factors (TFs) are proteins that bind to the DNA and help to control gene expression. We call the sequences to which they bind transcription factor binding sites (TFBSs), which are a type of cis-regulatory sequence. ...
Algebra 1 - Edublogs
Algebra 1 - Edublogs

... Directions: Identify each statement refers as true or false. ____ The ribosome and DNA interact to produce protein. ____ The ribosome puts amino acids together to produce a protein. ____ RNA acts as a messenger for DNA. ____ DNA leaves the nucleus. ____ RNA leaves the nucleus. ____ The order of amin ...
Fishy Code Slips
Fishy Code Slips

... Answers will be both yes and no for the first question. Yes, a change in type of tail fin can affect the ability of the fish to move properly. If a fish has no teeth, it will change what the fish can eat. ...
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RNA polymerase II holoenzyme

RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding genes in living cells. It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins.
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