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...  Bacteria need to respond swiftly to changes in their environment. - Induction: the synthesis of enzymes in response to the appearance of a specific substrate. This type of regulation is widespread in bacteria, as well as in unicellular eukaryotes (such as yeasts).  Fig. 12.6: summarizes the esse ...
PDF only
PDF only

... Biologists can search bibliographic databases via the Internet, using keyword queries that retrieve a large superset of relevant papers. Alternatively, they can navigate through hyperlinks between genome databanks and referenced papers. To extract the requisite knowledge from the retrieved papers, t ...
Investigation 1: Examining RNA-Seq data
Investigation 1: Examining RNA-Seq data

... focus on data from experiments that assess the RNA population in cells. This data can be used to help us identify exons and introns for the gene under study. All RNAs in the cell are collectively known as the 'transcriptome,’ as almost all RNA is produced by transcription from a DNA template. (In so ...
Attenuation regulation of amino acid biosynthetic operons in
Attenuation regulation of amino acid biosynthetic operons in

... ribosome translates the leader peptide, it prevents formation of the antiterminator structure, thereby promoting formation of the terminator (3:4), which causes premature termination of transcription (Fig. 1C). Thus, the ribosome plays the role of a mediator, sensing the concentration of charged tRN ...
Histone Modifications - Life Science Saga
Histone Modifications - Life Science Saga

... groups (CH3CO-) to lysines. Acetylation  DNA is stopped from getting further condensed Active transcription of the genes takes place ...
Protein synthesis: methionly-tRNAi recognizes the AUG start codon
Protein synthesis: methionly-tRNAi recognizes the AUG start codon

... Translation initiation usually occurs near the first AUG closest to the 5’ end of an mRNA eIF4A (helicase activity) →uses energy→ unwind RNA→complex move Kozak sequence: ACCAUGG UTR ...
Regulation of Gene Expression
Regulation of Gene Expression

... • Location: - - 80 bp • Function: It is the binding site for CTF1 (CAATbinding transcription factor) and C/EBP. The DNA-binding domain of TF1 is rich in basic Aas, and most likely it is in the alpha-helical conformation. C/EBP binds to DNA in a dimer known as leucine zipper. • Eukaryotes frequently ...
RNA Interference Regulates Gene Action
RNA Interference Regulates Gene Action

... genomic data that are being subjected to computational analysis to predict genes and other genetic elements. However, this is only the first step. As biologists we want to understand the functions of the predicted genes and their roles in the cell. Bioinformaticians are finding thousands—or tens of ...
NMR analysis of protein interactions
NMR analysis of protein interactions

... Not all RRM motifs, however, are involved in RNA binding; interactions with protein have been observed as well [44]. Another abundant single-stranded RNA binding motif is the KH domain, reviewed in [43]. A solution structure has been determined for the KH domain of splicing factor SF1 in complex wit ...
Searching for Novel Riboswitches in Newly Sequenced Genomes
Searching for Novel Riboswitches in Newly Sequenced Genomes

... Prokaryotic Transcription Termination by Riboswitches ...
p53
p53

... • amplification of proto-oncogene which increase the number of copies of oncogene inside the cell • point mutation in a proto-oncogene that change the gene’s protein product to a more active or more resistant to degradation than the normal protein. ...
Genetics Class- Ch. 10 Notes
Genetics Class- Ch. 10 Notes

... Transcription Occurs in three steps: • Initiation  promoter • Elongation  RNA polymerase adds nucleotides to growing RNA • Termination  Sequences in the DNA prompt the RNA polymerase to fall off, ending the transcript ...
Sec"on 8 - Small World Initiative
Sec"on 8 - Small World Initiative

... •  The  large  and  small  subunit  associate  only  in  the  presence  of  mRNA   •  The  mRNA  passes  through  a  “tunnel”  created  by  the  mature  ribosome   •  This  tunnel  contains  the  ac$ve  A,  P,  and  E  sites  where ...
LIPIDS
LIPIDS

... basic proteins (protamines, histones) forming chromatine. Nucleolus contains associated DNA and RNA Mitochondrial DNA (1-3%) in the mitochondria matrix – Structure of simple or double circular helix; does not form complex with proteins; MW << nuclear DNA – Function: Takes part in maintenance of the ...
PDF file
PDF file

... is 2 and 3 orders of magnitude greater than that obtained with a comparable concentration of poly(Glu4:Tyr1), the best phosphoprotein substrate tested. With artificial protein substrates, the specific activity of GST-PIR1 is about 5 orders of magnitude lower than that of PTP 1B (31), a typical tyros ...
Text Book of Molecular Biology
Text Book of Molecular Biology

... 2. The ‘beads on a string’ structure is organized into chromatin/chromosome 3. The mitotic chromosome has one centromere and two telomeres. P129 fig 6-12 The ends of the linear chromosomal DNA are protected from degradation and gradual shortening by the telomeres. The telomere consists of up to hund ...
Telomerase Is a True Reverse Transcriptase
Telomerase Is a True Reverse Transcriptase

... using deoxyribonucleotides as substrates and a sequence within the telomerase RNA subunit as a template, telomerase compensates for the limitations of conventional replication. Telomerase RNA subunits were later found in other ciliated protozoa [13-16], budding yeast [17, 18], and mammals [19, 20]. ...
Lecture 12
Lecture 12

... Ribosomes are large complexes of protein and ribosomal RNA . They consist of two subunits—one large and one small—whose relative sizes are generally given in terms of their sedimentation coefficients, or S (Svedberg) values. The prokaryotic 50S and 30S ribosomal subunits together form a 70S ribosome ...
Molecular Biology
Molecular Biology

... DNA replication is the process of duplicating the DNA sequence in the parent strand to produce an exact replica (daughter strand). Replication is semi-conservative: each one of the two parental strands serves as a template for the new strand synthesis; therefore, duplicated double helices contain on ...
molecular_general_theory_complete
molecular_general_theory_complete

... DNA replication is the process of duplicating the DNA sequence in the parent strand to produce an exact replica (daughter strand). Replication is semi-conservative: each one of the two parental strands serves as a template for the new strand synthesis; therefore, duplicated double helices contain on ...
dna replication
dna replication

... DNA replication is the process of duplicating the DNA sequence in the parent strand to produce an exact replica (daughter strand). Replication is semi-conservative: each one of the two parental strands serves as a template for the new strand synthesis; therefore, duplicated double helices contain on ...
Translation - Santa Susana High School
Translation - Santa Susana High School

... • large unit attaches after the union is made completing the initiation complex - translation begins – proteins called initiation factors bring the units together – energy from GTP is used in the formation of the unit ...
are mRNA
are mRNA

... modification & processing of mRNA in Eukaryotic Cell ...
Table S1 List of Ert1 targets (P 0.05 with enrichment values >1.8
Table S1 List of Ert1 targets (P 0.05 with enrichment values >1.8

... YKR097W ...
Real Time of PCR - KSU Faculty Member websites
Real Time of PCR - KSU Faculty Member websites

... application, PCR can amplify a small amount of template DNA (or RNA) into large quantities in a few hours. This is performed by mixing the DNA with primers on either side of the DNA (forward and reverse), Taq polymerase (of the species Thermus aquaticus, a thermophile whose polymerase is able to wit ...
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RNA polymerase II holoenzyme

RNA polymerase II holoenzyme is a form of eukaryotic RNA polymerase II that is recruited to the promoters of protein-coding genes in living cells. It consists of RNA polymerase II, a subset of general transcription factors, and regulatory proteins known as SRB proteins.
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