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What would we like to know about DNA and how do we obtain that
What would we like to know about DNA and how do we obtain that

... • Function and structure of RNA – Stabilizing forces for 3D structure – The different types of RNA – Catalysis acid base mechanism – General concepts of RNA World ...
Why don’t antibodies get rid of HIV?
Why don’t antibodies get rid of HIV?

... polymerase simultaneously transcribing two adjacent genes. ...
Protein synthesis - hrsbstaff.ednet.ns.ca
Protein synthesis - hrsbstaff.ednet.ns.ca

... Silent mutations have no effect on the operation of the cell. Usually silent mutations occur in the noncoding regions (introns) of DNA. Missense mutations occur when a change in the base sequence of DNA alters a codon (a code for a specific amino acid), resulting in the wrong amino acid being placed ...
INDUCTION OF ß-GALACTOSIDASE IN E.COLI
INDUCTION OF ß-GALACTOSIDASE IN E.COLI

... The machinery of RNA and protein synthesis needs a great amount of energy. The strictly controlled energy metabolism of living cells demands very selective and careful control of these processes. Enzyme induction and repression serve both in procaryotes and in eucaryotes as a main pathway of regulat ...
DNA and RNA
DNA and RNA

... Gene Regulation • Operators and promoters are DNA sequences in the operon that control when genes are turned on and off. – When the cell needs a certain protein, RNA polymerase attaches to the promoter and makes a messenger RNA that is translated into the needed protein. – When the cell no longer n ...
Jeopardy!!
Jeopardy!!

... Which type of RNA functions as a blueprint of the ...
DNA, RNA, & Protein Synthesis Notes (12.3)
DNA, RNA, & Protein Synthesis Notes (12.3)

... interpreters of the mRNA codon sequence.  At the middle of the folded strand, there is a three-base coding sequence called the anticodon.  Each anticodon is complementary to a codon on the mRNA. ...
Do Now: Wednesday, March 19
Do Now: Wednesday, March 19

... for the protein that is needed is unwound  Step 2: RNA polymerase (enzyme) uses the DNA to make a complementary strand of mRNA ...
Rapid communication A multiplex reverse transcriptase
Rapid communication A multiplex reverse transcriptase

... sample of plant material was weighted prior to being homogenized in 1.5-ml Eppendorf tubes containing 500 ll of Trizol (Gibco/ BRL) and incubated for 5 min at room temperature. The RNA was chloroform-extracted, isopropyl alcohol-precipitated and resuspended in H2O. Genomic DNA was removed by adding ...
DNA, RNA and Protein
DNA, RNA and Protein

... Therefore, each carbon atom can four covalent bonds with make ____ other types of atoms or additional carbons. ...
Supplementary Figure Legends (doc 60K)
Supplementary Figure Legends (doc 60K)

... extracellular ligand to a transmembrane G-protein coupled receptor (GPCR) alters the conformation of the associated heterotrimeric G protein, causing dissociation of the Gαs and Gβγ subunits and initiating a cascade of intracellular events. The subunit Gαs activates the adenylatecyclase enzyme (ADC ...
The Young Scholars Program - 1996
The Young Scholars Program - 1996

... ORF sequence to already known genes from other genomes or to other genes in E. coli’s genome. Within E. coli there are gene families of “paralogous” genes, which are not identical, but which have related sequence and function. For example there are 80 ABC transporter genes (genes involved in group t ...
Chapter 13 Viruses
Chapter 13 Viruses

... Chapter 13 viruses ...
PPS - VCU
PPS - VCU

... oligogonucleotide microarrays: implications for probe design and data analysis. ...
CHIP-seq and RNA-seq
CHIP-seq and RNA-seq

... 3. Map the reads to the Genome Build the database – bowtie2 b. Run the alignment - tophat a. ...
DNA and the Genome
DNA and the Genome

... • This enzyme is responsible for transcription. • RNA polymerase binds at the promoter and unwinds the DNA. ...
Cell Nucleus and Chromatin Structure
Cell Nucleus and Chromatin Structure

... the chromosome. Genes are transcribed from the DNA template by one of three RNA polymerases inside the nucleus. Transcription is initiated from a specialized promoter region upstream of the gene. Additional regulation by enhancers or repressor proteins may be required. RNA polymerase II is responsib ...
RNA Structure and Function
RNA Structure and Function

... along the RNA strand. The sugar/ phosphate string functions to “hold” the “letters” (N bases) in the proper order to spell out the recipe for the protein to be made. The N bases are the letters in the genetic code and the sugar/phosphate backbone is the “paper” on which the code is “written”. E. Typ ...
00_BioBackground
00_BioBackground

... • Regions in the DNA sequence encode instructions for the manufacture of proteins in the cell • Proteins are linear chains whose elements come from a set of 20 chemically active building blocks known as amino acids. • Each protein has a unique sequence of amino acids that is determined by a DNA sequ ...
Class Topics - Seneca High School
Class Topics - Seneca High School

... Codons ...
12.3 RNA and Protein Synthesis
12.3 RNA and Protein Synthesis

... – Similar to DNA replication ...
Functional Genomics
Functional Genomics

... genomes cannot be assigned function based on sequence similarity. • Genes sharing a common pattern of expression in many different experiments are likely to be involved in similar processes. – Gene A regulates Gene B, or vice versa – Gene A and Gene B are regulated by Gene C ...
E. Coli
E. Coli

... A.) GAL4, protein, a transcriptional activator that control expression of genes involved in galatose utilization. It contain two separable and functionally distinct domains that are both essential for activation of target gene expression: 1. N-terminal domain –responsible for specific DNA-binding ac ...
Chapter 4 Cellular Metabolism
Chapter 4 Cellular Metabolism

... where protein synthesis will occur. They lie across the __ribosome_ and wait for the ___transfer RNA to bring in the appropriate amino acids. The correct amino acids will be lined up because the tRNA bases are arranged in _anti-codons_ that are complementary to the __cocons_ of the bases of the mRNA ...
Presentation
Presentation

... with cytosine (C)  The process is ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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