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Figure 9.8
Figure 9.8

... can form short double-stranded regions – This secondary structure is due to complementary basepairing • A to U and C to G ...
Science - IIS E. Fermi
Science - IIS E. Fermi

... The process by which DNA achieves its control of cell life and function through protein synthesis is called gene expression. A gene is a DNA sequence that contains genetic information for one functional protein. Proteins are essential for the modulation and maintenance of cellular activities. The am ...
Enzymes: Principles of Catalysis
Enzymes: Principles of Catalysis

... 1. Regulation by Localization In eukaryotic cells, proteins can be targeted to specific locales: ER, Golgi, Nucleus, mitochondrion or secreted Specific signal sequences interact with other proteins at the target site KDEL: Endoplasmic reticulum KRKR: Nucleus Hydrophobic residues: Secretion (Golgi) ...
Chapter Two Line Title Here and Chapter Title Here and Here
Chapter Two Line Title Here and Chapter Title Here and Here

... In translation, the sequence of genetic information carried by mRNA is used by ribosomes to construct polypeptides with specific amino acid sequences. To understand how 4 DNA nucleotides can specify the 21 different amino acids commonly found in proteins requires an understanding of the genetic code ...
Eurofins` adaption and optimisation software “GENEius” in
Eurofins` adaption and optimisation software “GENEius” in

... be provided by the customer. Currently codon usage tables of over 35,000 organisms can be found in the Kazusa database. GENEius does not simply aim for a high codon adaption index (CAI), instead it harmonises the codons used. Frequently used codons from highly expressed genes are more often used in ...
Protein Synthesis
Protein Synthesis

... Each codon specifies which one of the 20 amino acids will be incorporated at the corresponding position along a polypeptide. ...
From DNA to Protein
From DNA to Protein

... • Synthesis is the same as the leading strand of DNA RNA Polymerase – Figure 7-8 • RNA is released so we can make many copies of the gene, usually before the first one is done – Can have multiple RNA polymerase molecules on a gene at a time Differences in DNA and RNA Polymerases • RNA polymerase add ...
Chapter 17 lecture notes
Chapter 17 lecture notes

... Each codon specifies which one of the 20 amino acids will be incorporated at the corresponding position along a polypeptide. ...
IPTG_09-10_8h
IPTG_09-10_8h

... Fig 1. Repression in the lac operon. (A) A RNA polymerase molecule binds to its promoter on the DNA to initiate transcription. (B) When a repressor molecule binds to its operator, it blocks part of the promoter and leads to repression. (C) A tetramerized repressor can bind to two operators at the sa ...
DNA sequencing File
DNA sequencing File

... sequence bases in a long length of DNA, let alone a large gene or an entire chromosome. • Instead, the DNA to be sequenced is cut into smaller fragments and these are sequenced. • A computer program then puts them in order by comparing overlapping sections of code. • Once the order of bases in DNA i ...
Name
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... Transcription & the Big picture 1. Fill-in-the-blank General Summary Like DNA, ribonucleic acid (RNA) is a ________________ acid—a molecule made of nucleotides linked together. RNA differs from DNA in three ways. First, RNA consists of a __________________ strand of nucleotides instead of the two st ...
Nucleic Acids
Nucleic Acids

... is synthesized, the ribosome reaches the “stop” codon: UGA, UAA, or UAG • There is no tRNA with an anticodon for the “stop” codons • Therefore, protein synthesis ends (termination) • The polypeptide is released from the ribosome and the protein can take on it’s 3-D structure (some proteins begin fol ...
NOTES: 13.1-13.2 - Protein Synthesis (powerpoint)
NOTES: 13.1-13.2 - Protein Synthesis (powerpoint)

... • How does it go from mRNA (copy of DNA) to amino acids (building blocks of proteins)?  A group of 3 mRNA bases makes up a “codon” (think of as a “code word”) ...
Week 5
Week 5

... Because DNA polymerase will bind only to double-stranded nucleic acid it is necessary to produce a hybrid DNA-RNA strand on the single-stranded template strand of DNA before replication of that sequence can begin. The RNA is referred to as primer RNA Procaryotes: ...
Insertional mutagenesis in zebrafish rapidly identifies genes
Insertional mutagenesis in zebrafish rapidly identifies genes

... heterozygous or non-transgenic • 24 mutants and 24 wildtype embryos were genotyped in most cases • If there are no recombinants and no other insertions, the insert is considered to be the cause of the mutation • RT-PCR or in situ hybridization was used as further evidence that the gene was disrupted ...
Spotted arrays
Spotted arrays

... • 8,734 PCR products from sequenced-verified clones from the UniGene database from NCBI, average length is greater than 500 nucleotides •Between 1-3 ug of DNA per well. Enough to fabricate 500 to 1,000 arrays • Corresponding clones available for purchase for further research ...
Protists
Protists

... PROTISTS- Chapter 28 “More about what they are not… than what they are” CHARACTERISTICS EUKARYOTES that AREN’T animals, plants, or fungi Primarily unicellular (Paramecium, Euglena); Some colonial (Volvox); some multicellular (Seaweed) Cilia or flagella at some time in life cycle Kingdom is POLYPHYLE ...
1 How-to-guide for accessing big data Franziska Denk 1. Go to http
1 How-to-guide for accessing big data Franziska Denk 1. Go to http

... NB: FPKM stands for Fragments Per Kilobase of transcript per Million mapped reads. Like RPKM (Reads Per Kilobase per Million mapped reads), it is a normalised measure of gene expression. It takes into account the length of the transcript (“per kilobase”) and the overall number of reads (“per million ...
Biotechnology IB Syllabus
Biotechnology IB Syllabus

...  Translation depends on complementary base pairing between codons on mRNA and anticodons on tRNA. 
 Applications and skills:  Application: Use of Taq DNA polymerase to produce multiple copies of DNA rapidly by the polymerase chain reaction (PCR).  Application: Production of human insulin in bacte ...
Regulation of Cell Cycle
Regulation of Cell Cycle

... Serine-threonine kinases-regulate function of proteins by phosphorylation of either Serine (S) or Threonine (T) Both subunits needed for the kinase activity of the complex ...
Sample
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... Answer: An acquired mutation is one that occurs in an organism after birth and throughout its life. It can be caused by, for example, too much exposure to radiation, which, over time, can cause a mutation or series of mutations that can lead to cancer. An inherited mutation is one in the germ line t ...
workshop-1
workshop-1

... - coding and non-coding sequence are slightly different in composition - some ‘possible’ splice sites are more likely than others scan genomic sequence … . . .CGTCGTATGGCTTCGATGTAGTACATCGGATCGGTATGGAATCATTTCAGTCGCTAGCTAGCCTAACGTATATAGCTAGGTAAGACTA. . ...
Cellular Division
Cellular Division

... Areas of DNA have readable sequences which code for genes Many parts of the genome are “junk DNA” Genes can have differences in the AT and GC, which form alleles If not detrimental, the mutation will continue If advantageous, the allele will become more common ...
A glucose-responsive transcription factor that regulates
A glucose-responsive transcription factor that regulates

... to efficiently store nutrients as fat during periods of abundant food supply as a safeguard against periodic famine (1). Coupled with dramatic changes in modern lifestyle and food consumption, these ‘‘thrifty genes’’ may now contribute to health defects suffered by as many as half of the American po ...
Presentation
Presentation

... A site binds to release factor ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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