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DNA methylation affects the cell cycle transcription of the CtrA global
DNA methylation affects the cell cycle transcription of the CtrA global

... (Marczynski, 1999). These results support the previous experiments indicating that hemimethylation of the ctrA P1 promoter is required for its full expression and further suggest that the effect is direct. To assess the role of CcrM in the direct regulation of ctrA P1 transcription, we generated a C ...
Some Biology that Computer Scientists Need for
Some Biology that Computer Scientists Need for

... • Only certain genes are “turned on” at any particular time. • When a gene is transcribed (copied to mRNA), it is said to be expressed. • The mRNA in a cell can be isolated. Its contents give a snapshot of the genes currently being expressed. • Correlating gene expressions with conditions gives hint ...
Notes for lecture 17: RNA Secondary Structure Prediction Adam
Notes for lecture 17: RNA Secondary Structure Prediction Adam

... tRNA during its formation. ...in Gene Regulation RNA secondary structure in the 5’ untranslated region of a messenger RNA can determine whether or not the rest of the mRNA will be transcribed or translated. An RNA that performs this function is called a “riboswitch.” An example of a riboswitch occur ...
Organic Molecules Notes
Organic Molecules Notes

... which are primarily responsible for transmitting characteristics through generations of living organisms show also characteristic symmetry breakings. Nucleic acids are macromolecules, which are formed by linear polymerization of certain units (nucleotides). According to the double helix model of JD ...
3.4 C: Transcription Quiz PROCTOR VERSION
3.4 C: Transcription Quiz PROCTOR VERSION

... The resulting RNA transcript is due to a single substitution in the DNA sequence. Distractor Rationale: This answer suggests the student may understand that a substitution in the DNA sequence will result in a change in the resulting RNA sequence, but does not understand that the given RNA transcript ...
CH 17 PPT
CH 17 PPT

... • Introns—noncoding sequences in DNA that intervene between coding sequences (exons); are initially transcribed but are not translated because they are excised before mRNA leaves the nucleus • Exons—coding sequences of a gene that are transcribed and expressed. • RNA splicing—RNA processing that rem ...


... ends" produced facilitate the insertion of foreign DNA into vector DNA. The foreign gene is sealed into the vector DNA by DNA ligase. When the plasmid replicates or the virus reproduces, the foreign gene is cloned. The polymerase chain reaction (PCR) uses the enzyme DNA polymerase to make multiple c ...
AP Biology Exam Review - Ed W. Clark High School
AP Biology Exam Review - Ed W. Clark High School

...  Transcription: mRNA, RNA polymerase  RNA processing: introns, exons, 5’cap, poly-A tail  Translation: mRNA, codon, tRNA, anticodon, ribosome, small ribosomal subunit, large ribosomal subunit, wobble, stop codon, start codon (Met), initiation, elongation, termination DNA Organization  Chromatin, ...
Homeotic genes - Teacherschoice
Homeotic genes - Teacherschoice

... Genes that affect embryo development by specifying the character of a body segment. The classic example is Antennapedia, a gene that, when mutated, causes a fruitfly to grow a leg in place of antenna. Homeotic genes are homeobox genes that are responsible for segment identity in metazoan organisms. ...
Homeotic genes
Homeotic genes

... Genes that affect embryo development by specifying the character of a body segment. The classic example is Antennapedia, a gene that, when mutated, causes a fruitfly to grow a leg in place of antenna. Homeotic genes are homeobox genes that are responsible for segment identity in metazoan organisms. ...
Gene Section FHL2 (four and a half LIM domains 2)
Gene Section FHL2 (four and a half LIM domains 2)

... placenta. It also induces osteoblast and myoblast differentiation. At cellular level, FHL2 participates in various processes, including cell survival, adhesion, motility, transcription and signal trans-duction. At molecular level, the LIM domains of FHL2 are double zinc finger motifs that physically ...
LECT34 RNAproc
LECT34 RNAproc

... from 5’-exonuclease activity. ...
protein synthesis - Science with Mrs Beggs
protein synthesis - Science with Mrs Beggs

... • Most are single stranded, therefore does not form a double helix. Although can fold and twist into itself. ...
Designing and making sgRNA constructs
Designing and making sgRNA constructs

... cloning backbone, the example guide sequence one base ‘G’ followed by 19 Ns, instead of the 20 Ns shown for the PX260/334 cloning backbones. This difference in oligo design was due to the requirement of human U6 promoter to have a ‘G’ base at the transcription start site. Hence, we recommend finding ...
Identify which nucleic acid (DNA or RNA) contains each of the
Identify which nucleic acid (DNA or RNA) contains each of the

... Problem 2 Condensation of the Components Provide the products for each of the following condensation reactions: a. ...
Lecture 12
Lecture 12

... 2. Avoid inverted repeat structures or inverted transcription units (see below) (Promoter X-Gene 1-nos3’:: nos3’-Gene 2-Promoter X or Y) 3. May use MARS to stabilize gene expression Matrix attachment regions (MARs) are operationally defined as DNA elements that bind specifically to the nuclear matri ...
Lecture 6 The connection between genes, proteins and metabolism
Lecture 6 The connection between genes, proteins and metabolism

... pathway responsible for biosynthesis of arginine ? YES   Beadle and Tatum went on to identify 3 different classes of mutants that could not synthesize arginine   Each mutant class had a metabolic block at a different step in the metabolic pathway that produces arginine   Because they isolated 3 c ...
Vocabulary Glossary - CTAE Resource Network
Vocabulary Glossary - CTAE Resource Network

... 8. Electroporation: Applying an electric current to a living surface in order to open pores through which something may pass 9. Ethidium Bromide: Fluorescent biological dye used to stain nucleic acids 10. Gel Electrophoresis: Technique to separate protein molecules of various sizes by moving them th ...
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Lecture slides

... Computing versus Biology • what computer science is to molecular biology is like what mathematics has been to physics ...... ...
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... (C) double-stranded ribonucleic acids (D) single-stranded DNA (E) double-stranded DNA. 25. Please choose the incorrect statement conceming natural macromolecules. (A) Starches are composed of monosaccharides. (B) Proteins are made of amino acids. (C) DNAs are composed of dideoxyribonucleotides. (D) ...
DNA & RNA
DNA & RNA

... rRNA and t-RNA images from © Pearson Education Inc, publishing as Pearson Prentice Hall. All rights reserved ...
Semliki Forest virus-based DNA expression vector
Semliki Forest virus-based DNA expression vector

... Figure 1 Construction of plasmid vectors. (a) Construction of pSFV3. The promoter sequence for the subgenomic RNA is located at the end of nsP4 gene and the BamHI–SmaI–XmaI polylinker cassette is positioned downstream of the subgenomic promoter. (b) To construct pSFV3-CMV-lacZ-pA, the cytomegaloviru ...
Answers to Quiz 4 BIol203 Fall 2013ppt
Answers to Quiz 4 BIol203 Fall 2013ppt

... C) (4pts) If you have a CCCCCUGGCU RNA binding protein in a given cell, then what are the most likely splice patterns you would observe in the mRNA for that cell? Be specific using exon numbers and letters. 1. 1, 2abc, 3, 5 2. 1, 2abc, 4, 5 3. 1, 2ab, 3, 5 4. 1, 2ab, 4, 5 Q2. (8pts) Sanger sequencin ...
HIV-1 Reverse Transcriptase
HIV-1 Reverse Transcriptase

... The dsDNA bound to the RT (2HMI) has a hybrid structure. The five base-pairs near the polymerase active site have a conformation similar to A-form DNA, while the nine basepairs towards the RNase active site have a conformation similar to B-form DNA. There is a significant bend involving the four ba ...
Slide 1
Slide 1

... • In-depth coverage of Computational Genomics  Algorithms for sequence analysis  Current applications, trends, and open problems ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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