: Determining DNA sequences
... • ddATP is a modified adenine base which has a coloured florescent marker attached. In has the added property of terminating the elongation if chosen instead of dATP • During the process all possible lengths of chain are produced. • Lengths are separated based on weight and analysed to give • The co ...
... • ddATP is a modified adenine base which has a coloured florescent marker attached. In has the added property of terminating the elongation if chosen instead of dATP • During the process all possible lengths of chain are produced. • Lengths are separated based on weight and analysed to give • The co ...
Biochem Option (D)
... B.8.1: Describe the structure of nucleotides and their condensation polymers ...
... B.8.1: Describe the structure of nucleotides and their condensation polymers ...
ChIP-seq
... There are many options for keeping samples cool during sonication: •Use the pulse mode to reduce heat buildup. •Put samples on ice along with the pulse mode. ...
... There are many options for keeping samples cool during sonication: •Use the pulse mode to reduce heat buildup. •Put samples on ice along with the pulse mode. ...
Energy Transfer in Living Things (Chapter 6)
... connected chains of nucleotides wound into a double helix • Double – two strands Helix- spiral • Looks like a twisted ladder ...
... connected chains of nucleotides wound into a double helix • Double – two strands Helix- spiral • Looks like a twisted ladder ...
A Comparison of Concentration Methods for Low Copy Number
... (LCN) DNA typing techniques are typically performed as part of, or following, the amplification process. These include but are not limited to: increased amplification cycles, reduced amplification reaction volumes, increased injection voltage and/or time, and post-amplification purification. Due to ...
... (LCN) DNA typing techniques are typically performed as part of, or following, the amplification process. These include but are not limited to: increased amplification cycles, reduced amplification reaction volumes, increased injection voltage and/or time, and post-amplification purification. Due to ...
For teachers: Get four colours of beads or rubber bands. You can
... The RULES 1. Read letters left to right in sets of three 2. Each three-letter code corresponds to an amino acid, such as “Leu” (see key) 3. T = U in the key* ...
... The RULES 1. Read letters left to right in sets of three 2. Each three-letter code corresponds to an amino acid, such as “Leu” (see key) 3. T = U in the key* ...
DNA Structure and Function Notes
... James Watson and Francis Crick were the first to put together a model of DNA’s structure. So, what does it look like? ...
... James Watson and Francis Crick were the first to put together a model of DNA’s structure. So, what does it look like? ...
DNA Replication
... Process continues until entire strand is copied Result is two strands of DNA Semi-Conservative Replication - Each strand is 50% new and 50% old DNA ...
... Process continues until entire strand is copied Result is two strands of DNA Semi-Conservative Replication - Each strand is 50% new and 50% old DNA ...
STUDY GUIDE FOR CHAPTER 12 – DNA Two Main Processes for
... c. _______________________________(enzymes, structural) d. _______________________________(DNA and RNA) 3. The building blocks of DNA and RNA are called _____________________________________ 4. Each building block consists of three main parts: a. _____________________________ Draw a nucleotide b. __ ...
... c. _______________________________(enzymes, structural) d. _______________________________(DNA and RNA) 3. The building blocks of DNA and RNA are called _____________________________________ 4. Each building block consists of three main parts: a. _____________________________ Draw a nucleotide b. __ ...
DNA Unit Test Study Guide extra added
... Rule’s. He found out that guanine always equals the amount of cytosine. He also found that adenine equals the amount of thymine. (Rosalind) Franklin – She used a process called X-ray diffraction to make images of DNA molecules. Her pictures showed that DNA has a spiral shape. (James)Watson and (Fran ...
... Rule’s. He found out that guanine always equals the amount of cytosine. He also found that adenine equals the amount of thymine. (Rosalind) Franklin – She used a process called X-ray diffraction to make images of DNA molecules. Her pictures showed that DNA has a spiral shape. (James)Watson and (Fran ...
IV.F.9 FILLING RECESSED 3` ENDS OF DOUBLE
... Generally, only one of the four dNTPs is labeled. Which dNTPs are added to the reaction depends on the sequence of the protruding 5' termini at the ends of the DNA; e.g., to fill in recessed 3' ends created by cleavage of DNA by EcoRI, only dATP and TTP need be present in the reaction: ...
... Generally, only one of the four dNTPs is labeled. Which dNTPs are added to the reaction depends on the sequence of the protruding 5' termini at the ends of the DNA; e.g., to fill in recessed 3' ends created by cleavage of DNA by EcoRI, only dATP and TTP need be present in the reaction: ...
Create the complementary strand for the following
... How does the cell use DNA to make proteins? -DNA is used as directions for making proteins. -DNA cannot leave the nucleus, so in order to make proteins, RNA must be made. -RNA is much like DNA however it only has one strand and instead of using the nucleotide Thymine (T) it uses the nucleotide ...
... How does the cell use DNA to make proteins? -DNA is used as directions for making proteins. -DNA cannot leave the nucleus, so in order to make proteins, RNA must be made. -RNA is much like DNA however it only has one strand and instead of using the nucleotide Thymine (T) it uses the nucleotide ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""