DNA Technology - University of Evansville Faculty Web sites
... Genetic engineers often use a plasmid vector to introduce new genes into plant cells Often the plasmid they use is from the soil bacterium, Agrobacterium tumefaciens When plants are infected with this bacterium in nature the develop tumors These are induced by the bacterium's Ti plasmid (Ti = tumor ...
... Genetic engineers often use a plasmid vector to introduce new genes into plant cells Often the plasmid they use is from the soil bacterium, Agrobacterium tumefaciens When plants are infected with this bacterium in nature the develop tumors These are induced by the bacterium's Ti plasmid (Ti = tumor ...
Nucleic Acids and the RNA World
... • Watson & Crick began to analyze the size and geometry of deoxyribose, phosphate groups, and nitrogenous bases. • Using things like bond angles, and measurements, they were able to devise 2.0nm probably represented the width of the helix, and .34 was likely the distance between bases stacked in the ...
... • Watson & Crick began to analyze the size and geometry of deoxyribose, phosphate groups, and nitrogenous bases. • Using things like bond angles, and measurements, they were able to devise 2.0nm probably represented the width of the helix, and .34 was likely the distance between bases stacked in the ...
DNA
... important problem to understanding the biochemistry of DNA replication. Since the two strands of double-helical DNA run in opposite (antiparallel) directions, continuous synthesis of two new strands at the replication fork would require that one strand be synthesized in the 5′ to 3′ direction while ...
... important problem to understanding the biochemistry of DNA replication. Since the two strands of double-helical DNA run in opposite (antiparallel) directions, continuous synthesis of two new strands at the replication fork would require that one strand be synthesized in the 5′ to 3′ direction while ...
recombinant DNA technology
... The procedure shown seeks to clone the human b-globin gene into a plasmid vector ...
... The procedure shown seeks to clone the human b-globin gene into a plasmid vector ...
DNA
... Free purine or pyrimidine bases are uncharged at physiologic pH Phosphoryl groups of nucleotides ensure that they bear a negative charge at physiologic pH nucleotides absorb light at a wavelength close to 260 nm The concentration of nucleotides and nucleic acids thus often is expressed in te ...
... Free purine or pyrimidine bases are uncharged at physiologic pH Phosphoryl groups of nucleotides ensure that they bear a negative charge at physiologic pH nucleotides absorb light at a wavelength close to 260 nm The concentration of nucleotides and nucleic acids thus often is expressed in te ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""