Manipulating and Analyzing DNA
... Today you will examine three extremely important aspects of biotechnology; restriction enzymes, recombinant DNA and gel electrophoresis. You will use two different websites to understand both topics. By the end of today you should be able answer the flooring questions: What are restriction enzymes? ...
... Today you will examine three extremely important aspects of biotechnology; restriction enzymes, recombinant DNA and gel electrophoresis. You will use two different websites to understand both topics. By the end of today you should be able answer the flooring questions: What are restriction enzymes? ...
DNA metabolism
... Recombination - linear sequence of DNA altered by cleavage and rejoining of chromosome (involves RecA protein) Repair of this type sometimes needed to reconstruct replication fork Human breast cancer genes (BRCA1 and BRCA2) produce proteins that interact with the human homolog of RecA, therefore the ...
... Recombination - linear sequence of DNA altered by cleavage and rejoining of chromosome (involves RecA protein) Repair of this type sometimes needed to reconstruct replication fork Human breast cancer genes (BRCA1 and BRCA2) produce proteins that interact with the human homolog of RecA, therefore the ...
Worksheet 1 (isolation)
... Besides the coding information (exons), DNA contains a lot of non-coding information (introns). During RNA processing these non-coding parts are removed. Before the synthesis of a protein starts, the corresponding RNA molecule is formed by RNA transcription. One strand of the DNA double helix is use ...
... Besides the coding information (exons), DNA contains a lot of non-coding information (introns). During RNA processing these non-coding parts are removed. Before the synthesis of a protein starts, the corresponding RNA molecule is formed by RNA transcription. One strand of the DNA double helix is use ...
DNA-RNA Review
... chain during protein synthesis Transfer RNA Structures found in the cytoplasm made of rRNA and proteins where protein synthesis happens Ribosomes ...
... chain during protein synthesis Transfer RNA Structures found in the cytoplasm made of rRNA and proteins where protein synthesis happens Ribosomes ...
MyTaq™ HS DNA Polymerase
... The leaves from plants such as Arabidopsis thaliana, corn and tomato are used for agricultural research and are a ready source of DNA without causing too much damage to the main plant. The use of ISOLATE II and MyTaq means that high quality DNA can be extracted from many leaves and then used in PCR ...
... The leaves from plants such as Arabidopsis thaliana, corn and tomato are used for agricultural research and are a ready source of DNA without causing too much damage to the main plant. The use of ISOLATE II and MyTaq means that high quality DNA can be extracted from many leaves and then used in PCR ...
How are animal proteins made from DNA?
... What is “transcription?” • A part of the DNA double helix within the nucleus is ________, cut by _______, and then copied onto a new ______ ______, called mRNA. This process is called ___________.” • Once the DNA is transcribed, the single strand moves from the ______ to a ________ in the _________ ...
... What is “transcription?” • A part of the DNA double helix within the nucleus is ________, cut by _______, and then copied onto a new ______ ______, called mRNA. This process is called ___________.” • Once the DNA is transcribed, the single strand moves from the ______ to a ________ in the _________ ...
DNA - Grant County Schools
... nitrogenous base, a sugar, and a phosphate group • In 1950, Erwin Chargaff reported that DNA composition varies from one species to the next, however that the nitrogen based are found in predictable ratios: ...
... nitrogenous base, a sugar, and a phosphate group • In 1950, Erwin Chargaff reported that DNA composition varies from one species to the next, however that the nitrogen based are found in predictable ratios: ...
PCR labwork 2 ENG
... a. Initial Denaturation for 10 minutes at 95°C: In this initiation step the hydrogen bonds are broken between the nucleotide base pairs and DNA strands separate from each other. b. Denature 30 seconds at 95°C: Continued denaturation of DNA double helix. c. Anneal primers for 30 seconds at 60°C: The ...
... a. Initial Denaturation for 10 minutes at 95°C: In this initiation step the hydrogen bonds are broken between the nucleotide base pairs and DNA strands separate from each other. b. Denature 30 seconds at 95°C: Continued denaturation of DNA double helix. c. Anneal primers for 30 seconds at 60°C: The ...
lecture15
... Restriction Fragment Length Polymorphism (RFLP) Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restri ...
... Restriction Fragment Length Polymorphism (RFLP) Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restri ...
theme one - Essentials Education
... The images have been cut out and pasted together to show the homologous pairs. Until recently this was done with scissors, it is now done with computer software They are generally numbered and arranged from longest to shortest. The sex chromosomes are bottom right of this photo. This is the male set ...
... The images have been cut out and pasted together to show the homologous pairs. Until recently this was done with scissors, it is now done with computer software They are generally numbered and arranged from longest to shortest. The sex chromosomes are bottom right of this photo. This is the male set ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""