• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Genes and DNA2012
Genes and DNA2012

... By the 1800s, asbestos was considered to be even more of a “miracle mineral” and was used in conjunction with the growth in industry. Using this readily available and inexpensive material in all manners of household construction offered many benefits, with no visible drawbacks, at the time. Asbestos ...
DNA and Chromosomes
DNA and Chromosomes

...  It is also very important for meiosis, which is the first step of sexual reproduction. ...
wind your way around your own dna - Ozias
wind your way around your own dna - Ozias

... Each of the 46 human chromosomes contains the DNA for hundreds or thousands of individual genes, the units of heredity. A GENE Each gene is a segment of double-stranded DNA that holds the recipe for making a specific molecule, usually protein. These recipes are spelled out in varying sequences of th ...
DNA/RNA
DNA/RNA

... – Chromosomal Inversions: an entire section of DNA is reversed. ...
GPVEC 2008 Biotech part 1
GPVEC 2008 Biotech part 1

... of disease-resistant wheat varieties by cross-breeding different wheat types until the desired disease resistance was present in a resulting new variety. ...
HIV and DNA replication answers
HIV and DNA replication answers

... The teacher should check the ideas of complementary base pairing, the use of DNA polymerase, semi-conservative methods, DNA template strands, and free nucleotides and their structure. ...


... over the internet ...
Make a DNA Model - Flinn Scientific
Make a DNA Model - Flinn Scientific

... have DNA. The DNA molecule is made up of several components—phosphate groups, deoxyribose sugars, and nitrogenous bases. The phosphate groups are the “backbone” of the DNA structure that link together the deoxyribose sugars (a pentose, or five-carbon monosaccharide). Each sugar is bonded with a nitr ...
Teacher practical Make your own protein Specification references
Teacher practical Make your own protein Specification references

... b The function of the protein depends on its shape, for example, the active site shape in an enzyme. If you change the shape, you change the function. Some mutations do not affect the function of a protein because not all of the amino acids will be involved in the bending and folding (or they do not ...
Southern Blotting DNA Fingerprinting
Southern Blotting DNA Fingerprinting

... Southern Blot • A Southern Blot identifies specific sequences of DNA • A Southern Blot may be used to determine a DNA fingerprint • A Southern Blot may be used in forsenic medicine ...
Steps in gene expression: comparison of
Steps in gene expression: comparison of

... In prokaryotic cells, genes do not have introns (no step 2) and transcription and translation are not separated in space and time (no step 3). ...
No Slide Title
No Slide Title

... Wellcome Trust Clinical Research Facilities ...
BACKGROUND CONCLUSIONS GOAL Define the protein YbfE’s role in helping
BACKGROUND CONCLUSIONS GOAL Define the protein YbfE’s role in helping

... show that overexpression of the ybfE gene is lethal. Sitedirected mutagenesis is being used to identify functional residues associated with the overexpression phenotype. recA ...
Assignment 1
Assignment 1

... Replicated copies of each chromosome are called sister chromatids and they are joined at the centromere. Homologues are referred to pair of chromosome, which might be non-identical ...
Answers
Answers

... Place these events in the correct order defining protein synthesis. a. b. c. d. e. f. g. h. i. j. k. l. m. n. o. ...
C16 DNA
C16 DNA

... The Mendelson-Stahl experiment showed that it was the semiconservative model that was most likely based on using isotope of nitrogen. All 6 billion bases in a human cell can be copied in a few hours. (Bacteria can copy their DNA in <1 hour). We have ~1000 X more DNA in our cells than bacteria. ~1 mi ...
From Bugs to Barcodes: Using Molecular Tools to Study
From Bugs to Barcodes: Using Molecular Tools to Study

... • In doing barcoding, we want to make the sequencing part as easy as possible – we want to be able to isolate DNA, amplify it and sequence it without having to clone the DNA • Thus it is important to use a haploid gene as our identifying gene. (If you used a diploid gene, you would get product with ...
Grimmer presentation
Grimmer presentation

... Business Center (DoI/ICB) contract number D15PC0002. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright annotation thereon. ...
Bacterial Genetics Summary
Bacterial Genetics Summary

Document
Document

... The result is selective purification and amplification of a particular target gene or DNA segment from a complex mixture of DNA molecules. The methods used to accomplish these and related tasks are collectively referred to as recombinant DNA technology or genetic engineering. ...
Radioisotopes in biology
Radioisotopes in biology

... -Autoradiography: To locate the position of a radiation source within a sample. The sample is placed on a photographic emulsion and an image is produced much as a normal photo. Weak β-emittors are sutiable (e.g. 3H, 35S) -Very sentitive method, exposure for days or longer ...
01 - Fort Bend ISD
01 - Fort Bend ISD

... chance that two people have eight repeats in location B is 1 in 50. The probability that two people have three repeats in location C is 1 in 200. What is the probability that two people would have matching DNA fingerprints for ...
A Short History of DNA Technology
A Short History of DNA Technology

DNA Transcription / Translation
DNA Transcription / Translation

...  B. RNA polymerase must first bind to a promoter sequence.  C. Transcription is always initiated at the start codon.  D. The 3’ end of the RNA molecule is produced first. ...
Plasmids - winterk
Plasmids - winterk

... They act as delivery vehicles, or vectors, to introduce foreign DNA into bacteria. Using plasmids for DNA delivery began in the 1970s when DNA from other organisms was first ‘cut and pasted’ into specific sites within the plasmid DNA. The modified plasmids were then reintroduced into bacteria. Decad ...
< 1 ... 452 453 454 455 456 457 458 459 460 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report