• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
The differences between Eukaryotes and
The differences between Eukaryotes and

... Introduction of human telomerase into such cells leads to reextension of the telomeres and results in normal immortalization of these progeric cell cultures. Clinical interventional studies using this strategy in humans are pending. Predictably, circulating lymphocytes of Hutchinson-Gilford children ...
Biology 321 Spring 2011 Answers to Assignment Set #5
Biology 321 Spring 2011 Answers to Assignment Set #5

... a. Neutral Sequence variation does not affect the Darwinian fitness of the organism. b. Yes. The alternative sequence variation exceeds 1% c. These sequence variations are not disease causing. You would want to focus your screen on the mutations in Table 2. d. It is in the third codon position so li ...
Product manual - biotechrabbit
Product manual - biotechrabbit

Teacher`s Guide for “Heredity” CT State Standards National Science
Teacher`s Guide for “Heredity” CT State Standards National Science

... 2. Pedigree
–
a
diagram
which
is
used
to
map
out
genetic
relationships
 within
a
family
line
 3. Chromosomes
–
an
organized
structure
of
DNA
that
contains
genes
 4. DNA
–
deoxyribonucleic
acid
–
found
in
nucleus
of
cells
which
 ...
Lectures 1-2 - Bilkent University Computer Engineering Department
Lectures 1-2 - Bilkent University Computer Engineering Department

... by “pairing up” with itself. This can have change its properties dramatically. ...
Chapter 8 Nucleotides and Nucleic acids
Chapter 8 Nucleotides and Nucleic acids

... Takes special solvent conditions or special sequences GC or 5methyl GC Some evidence for short stretches of Z in prokaryotes and Eukaryotes, but role in cell not known E. Unusual structures Bend in helix when more than 4 A’s on one strand (6 A’s make 18 degree bend) May be important in protein bindi ...
Alien Protein Synthesis
Alien Protein Synthesis

... In a process known as transcription (takes place in the nucleus) messenger RNA (mRNA) reads and copies the DNA. mRNA then takes the message out of the nucleus into the cytoplasm and finally to the ribosome (rRNA), the site of protein synthesis in a process known as translation. It is at the ribosome ...
Sequence Alignment Introduction
Sequence Alignment Introduction

... barcoding or phylogenetic comparisons of those species. The sequence includes the NADH dehydrogenase subunit 2 gene along with adjacent sequences that include some transfer RNA genes. ND2 gene is one of several genes that are often used for genetic fingerprinting in animals. It is suitable for th ...
Sec. 4.2 Quiz-like Thing
Sec. 4.2 Quiz-like Thing

... Cloning: involves DNA manipulation to produce multiple copies of a single gene or segment of DNA ...
DNA Marker - Faperta UGM
DNA Marker - Faperta UGM

... AFLP Markers  Most complex of marker technologies  Involves cleavage of DNA with two different enzymes  Involves ligation of specific linker pairs to the digested DNA  Subsets of the DNA are then amplified by PCR  The PCR products are then separated on acrylamide gel  128 linker combinations ...
Memorandum of Understanding and Agreement (MUA)
Memorandum of Understanding and Agreement (MUA)

... is to be sufficiently complete so as to provide committee members an understanding of what you intend to do and how you will do it. A summary or abstract of your methods and materials section will also be helpful. Please reference this discussion to appropriate NIH Guidelines and/or USDA/APHIS, and ...
Histone Methylation
Histone Methylation

... by mechanisms other than changes in the underlying DNA sequence – hence the name epi- (Greek: επί- over, above, outer) -genetics. It refers to functionally relevant modifications to the genome that do not involve a change in the nucleotide sequence. Examples of such modifications are DNA methylation ...
formativeassessment - the Biology Scholars Program Wiki
formativeassessment - the Biology Scholars Program Wiki

... Group work followed by report-out Jigsaw Concept maps Homework Projects Many others! ...
Genomic Digital Signal Processing
Genomic Digital Signal Processing

... Nearly all cells of an organism contain the genome. An exception is the red blood cell which lacks DNA. In procaryotes, the genome is found as a single circular piece. Eucaryotic genomes are often very long and are divided into packets called chromosomes. Different eucaryotes have different numbers ...
The Central Dogma Dry Lab
The Central Dogma Dry Lab

Nucleotides - Mrs Miller's Blog | Science Revision
Nucleotides - Mrs Miller's Blog | Science Revision

... RNA nucleotides are lined up against each base on the template strand, producing a complementary strand. As base pairing rules apply, this lining up will be the same as it appears on the coding strand- apart from U in RNA replacing T in DNA If a DNA template strand code reads ATTCGCGTTAAT, what woul ...
Zoo/Bot 3333
Zoo/Bot 3333

... Samples of DNA obtained from a fetus (F) and her parents (M and P) were cut by restriction enzyme R, then analyzed by gel electrophoresis followed by the Southern blot technique and hybridization with the radioactively labeled DNA probe designated “CF probe” in the above figure. Enzyme R has a six b ...
Genetics 314 – Spring 2007
Genetics 314 – Spring 2007

... different sequences for initiation and termination of transcription and translation. To make sure you get expression of a gene you need to have the proper promoter, leader and termination sequences to match the organisms enzymes involved in transcription and translation. 6. If a chemical was discove ...
Chapter 10: Molecular Biology of the Gene
Chapter 10: Molecular Biology of the Gene

... Definitive proof that DNA rather than Protein carries the hereditary information of life E. Coli bacteriophage: A virus that infects bacteria. Bacteriophages only contain a protein coat (capsid) and DNA. They wanted to find out whether the protein or DNA carried the genetic instructions to make ...
C2984Datasheet-Lot100
C2984Datasheet-Lot100

... strain are described below. The genotypes underlying these properties appear in parentheses. Blue/White Screening (F´ Δ(lacZ)M15): makes ω-fragment of β-gal; Δ(lacproAB) deletes the β-gal gene on the chromosome. pUC19 and similar plasmids code for the α-peptide of β-galactosidase (lacZ). The α-pepti ...
Introduction to Molecular Population Genetics
Introduction to Molecular Population Genetics

... Ecology is the study of how organisms interact in particular environments  Initially ecology concentrated on large scale systems involving animals and plants  However, the majority of living organisms on this planet are microorganisms and all environments ...
DNA
DNA

...  DNA is an antiparallel double helix: 5’ end of one strand is paired to 3’ end of other strand.  A & T and G & C are paired up by hydrogen bonds  Two strands are complementary to each other.  If you know sequence of one strand, can determine sequence of the other one. ...
Chapter 20 Practice Multiple Choice
Chapter 20 Practice Multiple Choice

... e. creation of products that will remove poisons from the human body ____ 19. Genetically engineered plants a. are more difficult to engineer than animals. b. include a transgenic rice plant that can help prevent vitamin A deficiency. c. are being rapidly developed, but traditional plant breeding pr ...
Genetic and dietary factors causing changes in gene activity through
Genetic and dietary factors causing changes in gene activity through

... Gains in cells treated with the chemotherapy agent DAC, which inhibits all three enzymes. It is currently not known how this is causing gains in methylation but they are likely to be very important for efficacy Supplementation with folic acid seems to give gains in methylation genome-wide, both for ...
RT-PCR - Faperta UGM
RT-PCR - Faperta UGM

... established protocols ...
< 1 ... 325 326 327 328 329 330 331 332 333 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report