• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
The methanol oxidation genes mxaFJGIR(S)ACKLD in
The methanol oxidation genes mxaFJGIR(S)ACKLD in

... (mxaACKLD) some, if not all, of which are involved in the insertion of calcium into the active site of MDH [8,9]. The mxaACKLD cluster has not been described in Paracoccus denitri¢cans, but immediately downstream of mxaI in this organism there is another gene (mxaR) and the 3P end of a putative ...
Using Fruit Flies to Investigate a Cancer Metastasis
Using Fruit Flies to Investigate a Cancer Metastasis

... and humans are responsible for the opposite phenotypes. With this result, we will, in the future, mutate specific amino acids within the WPD loop to confirm these are the amino acids that cause the phenotype. If hPRL-3 doesn’t have a oncogenic phenotype, then we know that the differences in the fly ...
Slide 1 - Schools
Slide 1 - Schools

... identical copies of a gene-carrying piece of DNA – Recombinant DNA is formed by joining DNA sequences from two different sources – One source contains the gene that will be cloned – Another source is a gene carrier, called a vector ...
Agro bacterium-mediated Transformation
Agro bacterium-mediated Transformation

If there are “CUES” listed within the question, please USE them and
If there are “CUES” listed within the question, please USE them and

... 6) Explain the concept of gene linkage. If you performed a test cross with a fruit fly that is heterozygous for two genes, how would you conclude that the two genes are linked? (CUES: crossing over, parental-type offspring, recombinant offspring, chromosome) 7) Use Figure 15.12 to explain non-disjun ...
Eukaryotic transcriptional control
Eukaryotic transcriptional control

... Heterodimeric transcription factors increase regulatory diversity and gene-control options (a) Many transcription factors (e.g. b-Zip and helix-loop-helix proteins) can form both homodimers or heterodimers with other members of the same class. (b) In the hypothetical example shown, transcription fa ...
Bacterial Transformation - Eastern Regional High School
Bacterial Transformation - Eastern Regional High School

... bacteria by uptake of foreign/new DNA ...
Chromosomal Mapping of Murine c-fes and c
Chromosomal Mapping of Murine c-fes and c

... DNA of the two progenitor strains A and B, followed by hybridization with a c-fes probe (7). The pattern obtained by using DNA digested with four different restriction enzymes suggests the existence of a single locus containing the murine c-fes gene (Fig. 1). Except for EcoRI, no other restriction e ...
A Biology Primer for Computer Scientists
A Biology Primer for Computer Scientists

... (zinc), and Co (cobalt). Individual atoms bond to each other to form more complex entities (molecules). There exists a number of binding mechanism, whose detailed study is not necessary in this context. Suffice it to say that the strength of different binding mechanisms varies substantially, and tha ...
Nucleic Acids and Chromatin
Nucleic Acids and Chromatin

... 1. DNA and RNA are polymers of nucleotides (polynucleotides). Nucleotides contain a base, a sugar and a phosphate. a. The base is either a purine (A & G), or a pyrimidine (T & C for DNA or U & C for RNA). In many cases the bases contain chemical modifications which may affect their function. Some of ...
Synthese der Oligonukleotide
Synthese der Oligonukleotide

... Since the discovery that RNA molecules fold into three dimensional objects, which can have catalytic properties the selection of RNA and DNA molecules with specific molecular recognition properties or catalytic capabilities has become a prime focus of research. These molecule are called ribozymes or ...
mc2 Genome_Organization
mc2 Genome_Organization

... – Sometimes processed pseudogenes insert into a location that is transcribed. Leads to a new fusion protein or a intronless gene. These are sometimes called “expressed processed pseudognes”. A whole group of them is expressed exclusively in the testes, with introncontaining homologues expressed in o ...
vertebrate genome evolution and function illuminated by chicken
vertebrate genome evolution and function illuminated by chicken

... Cold Spring Harbor course on Computational Genomics Nov. 10, 2007 ...


... Although this strain offers the advantage of a complete absence of a rigid cell wall and facile lysis, difficulties are experienced in achieving uniform growth and reproducible cell densities on account of a heterogeneous population of cells in liquid 'cultures. An IVTS from a wild type strain shoul ...
DNA Transcription & Translation
DNA Transcription & Translation

... 2. RNA is generally single-stranded 3. RNA contains uracil in place of thymine. ...
June 2007
June 2007

... Why are dominant mutations easier to detect than recessive mutations? (A) (B) (C) (D) ...
PCR of GFP - the BIOTECH Project
PCR of GFP - the BIOTECH Project

... 1. Label the PCR tube so that you can distinguish the samples in the tube. 2. Add 5 µl primer of each primer to each tube. If necessary, gently tap you tube on the counter to get all of the liquid to the bottom of the tube. 3. Add 10 µl GoTaq (green solution). Close the tubes and centrifuge briefly ...
SECTION I- BIOLOGY AND COMPUTER
SECTION I- BIOLOGY AND COMPUTER

... 23. Sequence of specific nucleotide that results in specific protein is called: a. mRNA b. Chromosomes c. Ligase d. Gene 24. In-vivo therapy is best related to: a. To cure hemophilia b. Cytokinin c. Develop into mature plants d. Mature Flowers 25. Change in the frequency of alleles occurring by chan ...
Practice Final Exam - mvhs
Practice Final Exam - mvhs

... penR gene-- This codes for a protein that breaks apart the antibiotic penicillin. This causes the penicillin to no longer function as an antibiotic. HLN gene-- This codes for the HLN protein which glows a fluorescent yellow color when it is exposed to UV light. stp90 gene-- This codes for the stp90 ...
AP Biology Deoxyribonucleic acid
AP Biology Deoxyribonucleic acid

... http://staff.um.edu.mt/acus1/3Molgen.htm ...
Cell Cyles
Cell Cyles

... an extra copy of the chromosome and the other cell to have no copy Upon fertilization, one embryo will have 3 copies and the other would have 1 copy (from the ‘normal’ parent) Cause of Down’s syndrome – they have 3 copies of chromosome 21 ...
Gene7-16
Gene7-16

... transposition like events Figure 16.8 Copy choice recombination occurs when reverse transcriptase releases its template and resumes DNA synthesis using a new template. Transfer between template strands is probably occurs directly, but is shown here in separate steps to illustrate the process. ...
lac Operon - Mediatech, Inc.
lac Operon - Mediatech, Inc.

... An operon is a unit of gene expression and a transcriptionally-regulated system. The lac operon is responsible for producing the proteins that control the uptake of lactose for use as a carbon energy source when glucose is not available to the cell. It consists of three structural genes and a repres ...
The Symbiotic Relationship of Science and Technology in the 21st
The Symbiotic Relationship of Science and Technology in the 21st

... A corn hybrid genetically altered to resist European corn borers was field tested by Ciba Seeds in 1992. Soybean seed is now available that has been genetically engineered to tolerate glyphosphate herbicides such as Roundup®, which kills virtually all vegetation (Monsanto, 1992). Monsanto has also d ...
1. Which of the following statements about homologous
1. Which of the following statements about homologous

... pregnancies will trigger an immune response. What is a possible explanation for why subsequent pregnancies could trigger an immune ...
< 1 ... 188 189 190 191 192 193 194 195 196 ... 652 >

Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report