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Unit 7 packet pt 5
Unit 7 packet pt 5

... Erwin Chargaff (1905–2002), an Austrian-American biochemist, investigated the ratio of nucleotide bases found in the DNA from a variety of organisms. From his research, as well as research by Rosalind Franklin and Maurice Wilkins, Watson and Crick developed the complementary base-pair rule during th ...
cell cycle
cell cycle

... the active site (which is made up of R groups with specific chemistry, i.e. hydrophobic). Enzyme rate is affected by:  pH (optimal for each enzyme),  temperature (optimal for each enzyme but in general increased temp means increased collisions so rate goes up initially; too much heat can denature ...
HL DNA_Jeopardy 2016
HL DNA_Jeopardy 2016

... During infection DNA is injected into the bacteria from the virus (bacteriophage), because DNA is the genetic material & DNA does not contain Sulfur atoms. ...
Modern Biotechnology. Connecting Innovations in Microbiology and Biochemistry to Engineering Fundamentals
Modern Biotechnology. Connecting Innovations in Microbiology and Biochemistry to Engineering Fundamentals

... Modern Biotechnology provides a much–needed introduction connecting the latest innovations in this area to key engineering fundamentals. With an unmatched level of coverage, this unique resource prepares a wide range of readers for the practical application of biotechnology in biopharmaceuticals, bi ...
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...  CODON: three sequential bases that code for a specific a.a. (20 a.a. total)  Amino acid are strung together to make a protein (primary structure)  Change DNA will change RNA which will change amino acids, which change protein ...
Biology Topic 2
Biology Topic 2

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Chapter 12

... 6. Explain how different organisms are used to massproduce proteins of human interest. 7. Explain how DNA technology has helped to produce insulin, growth hormone, and vaccines. 8. Explain how genetically modified (GM) organisms are transforming agriculture. 9. Describe the risks posed by the creati ...
Polymerase Chain Reaction (PCR) + Electrophoresis with agarose
Polymerase Chain Reaction (PCR) + Electrophoresis with agarose

... separation of the DNA fragments from your PCR reaction is accomplished by exploiting the mobilities with which different sized molecules are able to traverse the gel. Longer molecules migrate more slowly because they experience more drag within the gel. Because the size of the molecule affects its m ...
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DNA Barcoding and Its Applications

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... Cell will not enter the mitotic phase unless all the DNA has replicated. ...
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... lytic bacteriophage that is now carrying donor bacterial DNA due to an error in maturation during the lytic life cycle. specialized transduction: A DNA fragment is transferred from one bacterium to another by a temperate bacteriophage that is now carrying donor bacterial DNA due to an error in spon ...
Explain what genetic recombination is, why it is important and ho it
Explain what genetic recombination is, why it is important and ho it

... uptake – a cell absorbs plasmids from the environment.  Transduction – a virus acts as a vector to transfer pieces of foreign DNA from one cell to another. another  Conjugation – a temporary cytoplasmic bridge connects 2 cells so that DNA can be passed from one cell to the other: ...
Biology 1 Exam III F'04test.doc
Biology 1 Exam III F'04test.doc

... 15. An example of a type of chemical bond which involves a full separation of charge (transfer of electrons) is a/an: a. hydrogen bond. b. ionic bond. c. covalent bond. d. hydrophobic bond. e. electron bond. 16. A storage fat molecule is made up of: a. two fatty acids, glycerol, phosphate and anothe ...
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A-History-Of-Dna-Typing-And-Analysis-Criminology

... into databases. The Combined DNA Index System or CODIS is a collection of databases of DNA profiles obtained from evidence samples from unsolved crimes and from known individuals convicted of particular crimes (Butler). The FBI who oversees the database started CODIS in 1998. The main goal of the CO ...
DNA sequencing
DNA sequencing

... DNA As the negative charge increases with size, big DNA molecules would move more quickly  But bigger molecules move more slowly through the gel  Gives a steady and fine separation of DNA molecules by size  Molecules which differ by only one nucleotide in their length can be separated. ...
ppt link
ppt link

Lecture 27
Lecture 27

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Recombinant DNA Technology

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Review PowerPoint

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Chapter 17. Application of Recombinant DNA Technology in

... • In recombinant DNA, nucleotide sequences from two different sources, often two species, are combined in vitro into the same DNA molecule. • Methods for making recombinant DNA are central to genetic engineering, the direct manipulation of genes for practical purposes. • DNA technology has revolutio ...
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PD-PR-083: Laboratory protocol for manual

... for a minimum of 2 hours, or in a 50°C water incubator for a minimum of 1 hour. ...
Chapter 20
Chapter 20

... • In recombinant DNA, nucleotide sequences from two different sources, often two species, are combined in vitro into the same DNA molecule ...
Higher Human Biology Unit 1: Human Cells Homework Questions
Higher Human Biology Unit 1: Human Cells Homework Questions

... 2. A fragment of DNA was found to have 120 guanine bases and 60 adenine bases. What is the total number of sugar molecules in this fragment? A: 60 B: 90 C: 180 D: 360 3. If ten percent of the bases in a molecule of DNA are adenine, what is the ratio of adenine to guanine in the ...
Document
Document

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Transformation (genetics)



In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".
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