1. DNA SEQUENCER (Applied Biosystems, 3730xl DNA Analyzer)
... session (1 time-max: 1 hour) is available for new customer only by officer/staff in-charge before conducting the experiment. Additional training from third party is at customer’s own cost and arrangement upon approval from officer in-charge. Sample preparation & consumables are not included. Rental ...
... session (1 time-max: 1 hour) is available for new customer only by officer/staff in-charge before conducting the experiment. Additional training from third party is at customer’s own cost and arrangement upon approval from officer in-charge. Sample preparation & consumables are not included. Rental ...
DNA replication machinery
... molecules. The process of DNA replication is a fundamental process used by all living organisms as it is the basis for biological inheritance. As each DNA strand holds the same genetic information, both strands can serve as templates for the reproduction of the opposite strand. The template strand i ...
... molecules. The process of DNA replication is a fundamental process used by all living organisms as it is the basis for biological inheritance. As each DNA strand holds the same genetic information, both strands can serve as templates for the reproduction of the opposite strand. The template strand i ...
DNA and the Genome
... There are far more possible codons than amino acids. There are 64 (4 3 ) possible combinations of the four bases but only 20 amino acids occurring in nature. This has led to more than one codon coding for an amino acid. There are three codons that do not code for amino acids: UGA, UAA and UAG. The o ...
... There are far more possible codons than amino acids. There are 64 (4 3 ) possible combinations of the four bases but only 20 amino acids occurring in nature. This has led to more than one codon coding for an amino acid. There are three codons that do not code for amino acids: UGA, UAA and UAG. The o ...
Bergey`s Manual
... DNA fingerprinting: Number and sizes of DNA fragments (fingerprints) produced by RE digests are used to determine genetic similarities. Ribotyping: rRNA sequencing Polymerase chain reaction (PCR) can be used to amplify a small amount of microbial DNA in a sample. The Fig 10.14: Electrophoresis ...
... DNA fingerprinting: Number and sizes of DNA fragments (fingerprints) produced by RE digests are used to determine genetic similarities. Ribotyping: rRNA sequencing Polymerase chain reaction (PCR) can be used to amplify a small amount of microbial DNA in a sample. The Fig 10.14: Electrophoresis ...
Gene Section NBS1 (Nijmegen breakage syndrome 1) Atlas of Genetics and Cytogenetics
... The 754 amino acid protein is called nibrin; predicted MW 85 kDa, 95 kDa by SDS-PAGE; contains in Nterm a forkhead associated domain (amino acids 24100) and a breast cancer domain (BRCT; amino acids 105-190), both domains being found in the various DNA damage responsive cell cycle checkpoint protein ...
... The 754 amino acid protein is called nibrin; predicted MW 85 kDa, 95 kDa by SDS-PAGE; contains in Nterm a forkhead associated domain (amino acids 24100) and a breast cancer domain (BRCT; amino acids 105-190), both domains being found in the various DNA damage responsive cell cycle checkpoint protein ...
1.d Standard curve construction and validation of the C t
... Supporting information 1: Complete experimental protocol 1.a DNA template Fresh tissue (entire brain and 0.2g muscle) samples were dissected out immediately after sampling and stored at -80°C until DNA extraction. The DNA was extracted using a Wizard® Genomic DNA Purification Kit (Promega) following ...
... Supporting information 1: Complete experimental protocol 1.a DNA template Fresh tissue (entire brain and 0.2g muscle) samples were dissected out immediately after sampling and stored at -80°C until DNA extraction. The DNA was extracted using a Wizard® Genomic DNA Purification Kit (Promega) following ...
Restriction Digest of pAMP and pKAN
... It is important at this stage of our experimental procedure that we need to onfirm that Hind III and BamH I have digested the original plasmids and that we have the correct restriction fragments. Gel electrophoresis is a procedure commonly used to separate fragments of DNA according to molecular siz ...
... It is important at this stage of our experimental procedure that we need to onfirm that Hind III and BamH I have digested the original plasmids and that we have the correct restriction fragments. Gel electrophoresis is a procedure commonly used to separate fragments of DNA according to molecular siz ...
Genetic Transformation computer exercise v02 r01
... an algorithm (a step-by-step procedure) to compare the order of nucleotide bases in the sequences and then lines them up so that the number of identical bases is maximized. The alignment program will point out those bases that are identical (indicated by an asterisk - ), those that are similar (:), ...
... an algorithm (a step-by-step procedure) to compare the order of nucleotide bases in the sequences and then lines them up so that the number of identical bases is maximized. The alignment program will point out those bases that are identical (indicated by an asterisk - ), those that are similar (:), ...
Caffeine Metabolism Gene Zephyr and Walsh (2015)
... asked to identify the restriction sites for both ApaI and SacI, describe the sizes that will occur when the enzymes do or do not cut, and then draw out the gels for homozygotes and a heterozygote. In this way, students can form an educated guess about the variety of results they may achieve and make ...
... asked to identify the restriction sites for both ApaI and SacI, describe the sizes that will occur when the enzymes do or do not cut, and then draw out the gels for homozygotes and a heterozygote. In this way, students can form an educated guess about the variety of results they may achieve and make ...
Data for two plasmid isolation techniques, the rapid alkaline extraction... Nucleic Acids Res. 7: 1513-1523) and the rapid boiling technique...
... Often the detrimental trait of the second component itself acts as a forcing marker (E.G. colonial morphology, slow growth due to cytochrome deficiency). But this is not true of all traits for which shelter in the heterokaryon may be desired (e.g. sterility mutants, unstable alleles). The forced het ...
... Often the detrimental trait of the second component itself acts as a forcing marker (E.G. colonial morphology, slow growth due to cytochrome deficiency). But this is not true of all traits for which shelter in the heterokaryon may be desired (e.g. sterility mutants, unstable alleles). The forced het ...
Prostate cancer cell lines case study on cell cycle map
... and the G1-‐S checkpoint. Two interpreta1ons are possible for the LNCAP cells: -‐ most cells are expressing genes of the G1/S checkpoint. The LNCAP cells could try to overpass the checkpoint with l ...
... and the G1-‐S checkpoint. Two interpreta1ons are possible for the LNCAP cells: -‐ most cells are expressing genes of the G1/S checkpoint. The LNCAP cells could try to overpass the checkpoint with l ...
Constructing and Screening a Recombinant DNA Library
... a) When you construct the yeast genomic library in E. coli, what type of yeast would you choose as the donor for the genomic DNA? A wildtype yeast strain or a lysine prototroph. b) Once the DNA is isolated from the yeast cells, what would be your next step in preparing the DNA for use in constructin ...
... a) When you construct the yeast genomic library in E. coli, what type of yeast would you choose as the donor for the genomic DNA? A wildtype yeast strain or a lysine prototroph. b) Once the DNA is isolated from the yeast cells, what would be your next step in preparing the DNA for use in constructin ...
Teacher resource 1
... Ser-Cys-Ile-Glu-Asn-Cys-Asp-Arg-Tyr-Arg-Lys-Gly-Glu-Arg-Leu-Arg SCIENCDRYRKGERLR ...
... Ser-Cys-Ile-Glu-Asn-Cys-Asp-Arg-Tyr-Arg-Lys-Gly-Glu-Arg-Leu-Arg SCIENCDRYRKGERLR ...
Fast, high-resolution DNA sizing with the fragment analyzer system
... Fast, High-Resolution DNA Sizing with the Fragment Analyzer™ System Accurately size DNA up to 50 kb in 1 hour for large-insert SMRTbell™ libraries The Fragment Analyzer™ instrument is a fast, high-resolution benchtop capillary electrophoresis (CE) platform that utilizes proprietary markers to accura ...
... Fast, High-Resolution DNA Sizing with the Fragment Analyzer™ System Accurately size DNA up to 50 kb in 1 hour for large-insert SMRTbell™ libraries The Fragment Analyzer™ instrument is a fast, high-resolution benchtop capillary electrophoresis (CE) platform that utilizes proprietary markers to accura ...
Nick Translation DNA Labeling Systems
... 10 ng/µl in TE Buffer (10mM Tris Buffer, pH 8.0, 1mM EDTA) ...
... 10 ng/µl in TE Buffer (10mM Tris Buffer, pH 8.0, 1mM EDTA) ...
Genetics 314 – Spring 2004
... Name: __________________________ 2. The Mars rovers make a surprising discovery, not only did they find evidence of water they also found evidence of life on Mars! Surprisingly Mars life also had DNA but the DNA replicated differently. It was found to replicate in a conservative manner. How does th ...
... Name: __________________________ 2. The Mars rovers make a surprising discovery, not only did they find evidence of water they also found evidence of life on Mars! Surprisingly Mars life also had DNA but the DNA replicated differently. It was found to replicate in a conservative manner. How does th ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".