Molecular Genetics
... base pairing, the two sides of the molecule are mirror images of each other • 7. each strand contains the information needed to produce the second strand • 8. Watson and Crick proposed an early model of replication in their first paper describing the structure of DNA ...
... base pairing, the two sides of the molecule are mirror images of each other • 7. each strand contains the information needed to produce the second strand • 8. Watson and Crick proposed an early model of replication in their first paper describing the structure of DNA ...
DNA Replication
... Power Standard (s) Reference: Standard 6- Nucleic Acids and Protein Synthesis State Standard: Goal 1.1: Understand Systems, Order, and Organization 9-10.B.1.1.1 Explain the scientific meaning of system, order, and organization. 9-10.B.1.1.2 Apply the concepts of order and organization to a given sys ...
... Power Standard (s) Reference: Standard 6- Nucleic Acids and Protein Synthesis State Standard: Goal 1.1: Understand Systems, Order, and Organization 9-10.B.1.1.1 Explain the scientific meaning of system, order, and organization. 9-10.B.1.1.2 Apply the concepts of order and organization to a given sys ...
DNA and protein synthesis
... o rRNA is produced in the nucleolus and makes up the ribosomes, found in the cytoplasm How does DNA replication work? o DNA unzips when helicase breaks hydrogen bonds between the bases. o DN polymerase pairs complementary bases to those on the original or parent strand. This produces the daughter ...
... o rRNA is produced in the nucleolus and makes up the ribosomes, found in the cytoplasm How does DNA replication work? o DNA unzips when helicase breaks hydrogen bonds between the bases. o DN polymerase pairs complementary bases to those on the original or parent strand. This produces the daughter ...
General Microbiology Lecture Twelve Identification of Bacteria
... bacterium that does not produce visible results. The total of all the proteins expressed by genes can be detected by isolating chemically all the protein and separating them by electrophoresis using polyacrilamide gel. (PAGE). • When the polyacrilamide is stained with a dye specific for protein a pa ...
... bacterium that does not produce visible results. The total of all the proteins expressed by genes can be detected by isolating chemically all the protein and separating them by electrophoresis using polyacrilamide gel. (PAGE). • When the polyacrilamide is stained with a dye specific for protein a pa ...
Genetic Engineering Notes - Teacher Copy
... o DNA molecules used for transformation of animal and plant cells contain marker genes. o DNA molecules can be constructed with two ends that will sometimes recombine with specific sequences in the host chromosome. o The host gene normally found between those two sequences may be lost or replaced wi ...
... o DNA molecules used for transformation of animal and plant cells contain marker genes. o DNA molecules can be constructed with two ends that will sometimes recombine with specific sequences in the host chromosome. o The host gene normally found between those two sequences may be lost or replaced wi ...
DNA/Protein Synthesis Study Guide
... If one strand of DNA has the base sequence AATCCG, what will be the corresponding base sequence of its complementary strand? ...
... If one strand of DNA has the base sequence AATCCG, what will be the corresponding base sequence of its complementary strand? ...
A Comparison of Concentration Methods for Low Copy Number
... A commonly encountered hurdle in the processing of forensic DNA samples is the amount of DNA available for STR genotyping. This can be a function of either the sample itself, the collection of DNA from the sample, or the DNA extraction process. Standard low copy number (LCN) DNA typing techniques ar ...
... A commonly encountered hurdle in the processing of forensic DNA samples is the amount of DNA available for STR genotyping. This can be a function of either the sample itself, the collection of DNA from the sample, or the DNA extraction process. Standard low copy number (LCN) DNA typing techniques ar ...
Note. The efficiency of Benzonase treatment can be determined by
... 2. If dead cells/debris are visible in viral supernatant, centrifuge tube at 1000 rpm for 5 min. Remove the supernatant and transfer to a fresh sterile tube, and discard the pellet. 3. Optional: Concentrate the virus and/or filter the virus using 0.45 um filter units. 4. Treat viral supernatant with ...
... 2. If dead cells/debris are visible in viral supernatant, centrifuge tube at 1000 rpm for 5 min. Remove the supernatant and transfer to a fresh sterile tube, and discard the pellet. 3. Optional: Concentrate the virus and/or filter the virus using 0.45 um filter units. 4. Treat viral supernatant with ...
File
... Recombinant DNA is made by taking short pieces of DNA from one organism and joining it to the DNA of a completely different organism. Once the DNA is made, it can be placed back into a living cell in a process called transformation. ...
... Recombinant DNA is made by taking short pieces of DNA from one organism and joining it to the DNA of a completely different organism. Once the DNA is made, it can be placed back into a living cell in a process called transformation. ...
Cloning Power Point
... multiple copies of that gene using bacterial plasmids, selfreplicating extra-chromosomal circular DNA molecules, that are distinctly different from the normal bacterial genome. Genes and other chromosomes are copied to make enough samples for further study. In order to clone a gene, a fragment of DN ...
... multiple copies of that gene using bacterial plasmids, selfreplicating extra-chromosomal circular DNA molecules, that are distinctly different from the normal bacterial genome. Genes and other chromosomes are copied to make enough samples for further study. In order to clone a gene, a fragment of DN ...
Document
... • Engineer crops to be resistant to cold or insects • Engineer crops to produce vitamins or medicines • Engineer bacteria to produce medicines or enzymes ...
... • Engineer crops to be resistant to cold or insects • Engineer crops to produce vitamins or medicines • Engineer bacteria to produce medicines or enzymes ...
Bio07_TR__U04_CH13.QXD
... all bacteria take in the plasmid. Scientists can identify those bacteria that carry the plasmid because the plasmid also carries a genetic marker. Usually, the genetic marker is a gene that gives the bacteria resistance to a certain antibiotic. Plant cells can also be transformed. Scientists insert ...
... all bacteria take in the plasmid. Scientists can identify those bacteria that carry the plasmid because the plasmid also carries a genetic marker. Usually, the genetic marker is a gene that gives the bacteria resistance to a certain antibiotic. Plant cells can also be transformed. Scientists insert ...
Genetic Engineering Notes
... o Ex: deactivating the gene responsible for the ripening of tomatoes. This new gene can then be inserted into tomato DNA to give them a longer shelf life. ...
... o Ex: deactivating the gene responsible for the ripening of tomatoes. This new gene can then be inserted into tomato DNA to give them a longer shelf life. ...
ChIP-seq
... There are many options for keeping samples cool during sonication: •Use the pulse mode to reduce heat buildup. •Put samples on ice along with the pulse mode. ...
... There are many options for keeping samples cool during sonication: •Use the pulse mode to reduce heat buildup. •Put samples on ice along with the pulse mode. ...
Supplemental Material
... PacI and cloned in the AscI -PacI sites of the pMCB17apx vector (EFIMOV 2003) resulting in the plasmid pMCB17apx-ATM2000. This plasmid was used to transform the wild type recipient strain GR5. Transformants were tested for their ability to grow in YAG and homologous recombination of this plasmid int ...
... PacI and cloned in the AscI -PacI sites of the pMCB17apx vector (EFIMOV 2003) resulting in the plasmid pMCB17apx-ATM2000. This plasmid was used to transform the wild type recipient strain GR5. Transformants were tested for their ability to grow in YAG and homologous recombination of this plasmid int ...
viruses and bacteria
... Virus particles bind to wall of suitable host. Viral genetic material enters cell cytoplasm. Viral protein molecules are assembled into coats; DNA is packaged inside. ...
... Virus particles bind to wall of suitable host. Viral genetic material enters cell cytoplasm. Viral protein molecules are assembled into coats; DNA is packaged inside. ...
Chapter 16 Outline
... How Are The Four Stages Of Genetic Engineering Experiments Performed? Stage 1 - DNA Cleavage ...
... How Are The Four Stages Of Genetic Engineering Experiments Performed? Stage 1 - DNA Cleavage ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".