Biotechnology - (www.ramsey.k12.nj.us).
... A DNA construct can be injected into 0.5-day-old fertilized embryos to produce transgenic mice containing the DNA in every cell. The DNA construct typically consists of a gene of interest that is expressed in a certain range of tissues. The resulting mice are mated and the DNA construct is then tra ...
... A DNA construct can be injected into 0.5-day-old fertilized embryos to produce transgenic mice containing the DNA in every cell. The DNA construct typically consists of a gene of interest that is expressed in a certain range of tissues. The resulting mice are mated and the DNA construct is then tra ...
Discovery of the DNA molecule
... Deoxyribonucleic Acid (DNA) Students will be successful when they can…….. ...
... Deoxyribonucleic Acid (DNA) Students will be successful when they can…….. ...
DNA Unit Test Corrections
... 26. If a protein has 10 codons, how many amino acids are there in the protein?_____________ 27. How many amino acids are used by the human body to make proteins?________ 28. Why is the shape of a protein important? _______________________________________ _____________________________________________ ...
... 26. If a protein has 10 codons, how many amino acids are there in the protein?_____________ 27. How many amino acids are used by the human body to make proteins?________ 28. Why is the shape of a protein important? _______________________________________ _____________________________________________ ...
Linkage
... other genes for transfer of genetic material – F+ cells attach to F- cells w/ pilus; – DNA unwinds, and a ss DNA is transferred from the donor to the recipient cell. – DNA synthesis occurs in both, making ds DNA. – Genetic recombination: replacement of old genes ...
... other genes for transfer of genetic material – F+ cells attach to F- cells w/ pilus; – DNA unwinds, and a ss DNA is transferred from the donor to the recipient cell. – DNA synthesis occurs in both, making ds DNA. – Genetic recombination: replacement of old genes ...
DNA to Proteins….a REVIEW
... Below you are given the DNA, mRNA, or the Amino Acid. Fill in the blanks. For some of the blanks you may have to work backwards. *Remember you can only use the mRNA in the Amino Acid chart!!! *Pick one of the Amino Acids and use that codon to fill in the mRNA. ...
... Below you are given the DNA, mRNA, or the Amino Acid. Fill in the blanks. For some of the blanks you may have to work backwards. *Remember you can only use the mRNA in the Amino Acid chart!!! *Pick one of the Amino Acids and use that codon to fill in the mRNA. ...
`Quickie` Quikchange mutagenesis (Stratagene)
... Things to consider: a) The most frequent problem, not surprisingly, is ‘no colonies’. Almost always, if you get colonies (even just a couple), you’re going to get your mutant. To facilitate screening for mutants, you may incorporate a ‘silent’ (one that doesn’t change the coding) restriction site ( ...
... Things to consider: a) The most frequent problem, not surprisingly, is ‘no colonies’. Almost always, if you get colonies (even just a couple), you’re going to get your mutant. To facilitate screening for mutants, you may incorporate a ‘silent’ (one that doesn’t change the coding) restriction site ( ...
Restriction Enzymes
... unaffected when exposed to phages • Luria hypothesized that these bacteria had some type of primitive immune system that restricted phage growth • Luria’s hypothesis was later confirmed by several teams of researchers when they discovered restriction enzymes which cut up phage DNA when it is injecte ...
... unaffected when exposed to phages • Luria hypothesized that these bacteria had some type of primitive immune system that restricted phage growth • Luria’s hypothesis was later confirmed by several teams of researchers when they discovered restriction enzymes which cut up phage DNA when it is injecte ...
Linkage
... Mechanisms of horizontal gene transmission • Conjugation – Bacteria make direct contact with pilus – Transfer genes directly – Both related and unrelated partners ...
... Mechanisms of horizontal gene transmission • Conjugation – Bacteria make direct contact with pilus – Transfer genes directly – Both related and unrelated partners ...
4.4 Genetic Engineering and Biotechnology
... Outline three outcomes of the sequencing of the complete human genome. ...
... Outline three outcomes of the sequencing of the complete human genome. ...
Notes
... • Recombinant DNA technology – A new DNA molecule created by combining DNA from two different sources • Used to create recombinant DNA to study individual genes and genetically engineered organisms and in the treatment of certain diseases • Requires large quantities (how done – see next slide) ...
... • Recombinant DNA technology – A new DNA molecule created by combining DNA from two different sources • Used to create recombinant DNA to study individual genes and genetically engineered organisms and in the treatment of certain diseases • Requires large quantities (how done – see next slide) ...
PLASMIDS AND RESTRICTION ENZYMES
... In the early 1950s, scientists observed that certain strains of E. coli, a common bacterium found in the human gut, were resistant to infection by bacteriophages (viruses that infect bacteria by injecting their DNA into the cell and commandeering the host cell’s molecular processes to make more bact ...
... In the early 1950s, scientists observed that certain strains of E. coli, a common bacterium found in the human gut, were resistant to infection by bacteriophages (viruses that infect bacteria by injecting their DNA into the cell and commandeering the host cell’s molecular processes to make more bact ...
Topic 20 revision notes - Mr Cartlidge`s Saigon Science Blog
... insertion of human DNA into bacterial plasmid DNA using DNA ligase to form a recombinant plasmid ...
... insertion of human DNA into bacterial plasmid DNA using DNA ligase to form a recombinant plasmid ...
analysis
... 1. This method was devised by Sanger and used dideoxynucleotides to terminate chain elongation during DNA synthesis B. Purpose 1. Use sequence to deduce amino acid sequence of proteins 2. Find restriction sites 3. Find introns a) Compare genomic DNA to cDNA 4. Find DNA structures a) Inverted repeats ...
... 1. This method was devised by Sanger and used dideoxynucleotides to terminate chain elongation during DNA synthesis B. Purpose 1. Use sequence to deduce amino acid sequence of proteins 2. Find restriction sites 3. Find introns a) Compare genomic DNA to cDNA 4. Find DNA structures a) Inverted repeats ...
Structures of the bacteriophage Sf6 terminase large subunit reveal a
... capsid fueled by ATP hydrolysis. When an appropriate amount of DNA is inserted, the terminase large subunit makes a second cut on DNA, and brings the remaining DNA to next capsid for next packaging cycle. The terminase large subunit contains an N-terminal ATPase domain and a C-terminal nuclease doma ...
... capsid fueled by ATP hydrolysis. When an appropriate amount of DNA is inserted, the terminase large subunit makes a second cut on DNA, and brings the remaining DNA to next capsid for next packaging cycle. The terminase large subunit contains an N-terminal ATPase domain and a C-terminal nuclease doma ...
Biology 105
... DNA as genetic material • Transformation - process of changing the genetic makeup of an organism by an another organism. ...
... DNA as genetic material • Transformation - process of changing the genetic makeup of an organism by an another organism. ...
Session 4 - OpenWetWare
... mass production through culturing. Vectors maintained in strains and isolated for use need to be prepared to accept insert DNA. This is done by restriction digestion. Restriction digestion will cut a circular plasmid to make it linear; leaving ends which are compatible with other pieces of DNA cut w ...
... mass production through culturing. Vectors maintained in strains and isolated for use need to be prepared to accept insert DNA. This is done by restriction digestion. Restriction digestion will cut a circular plasmid to make it linear; leaving ends which are compatible with other pieces of DNA cut w ...
DNA Replication Practice Worksheet
... of a living organism and determine the organism's characteristics. When the cell reproduces, it has to pass all of this information on to the daughter cells. Before a cell can reproduce, it must first replicate, or make a copy of, its DNA. Where DNA replication occurs depends upon whether the cells ...
... of a living organism and determine the organism's characteristics. When the cell reproduces, it has to pass all of this information on to the daughter cells. Before a cell can reproduce, it must first replicate, or make a copy of, its DNA. Where DNA replication occurs depends upon whether the cells ...
Protocol for Phage T1-Resistant TransforMax™ EC100™
... 2. Prepare 1 ml of SOC medium (do not include antibiotic in the medium) for each electroporation to be performed. This medium will be used for post-electroporation outgrowth of transformed cells. Maintain the medium at room temperature. 3. Pre-chill electroporation cuvettes and 1.5 ml tubes on ...
... 2. Prepare 1 ml of SOC medium (do not include antibiotic in the medium) for each electroporation to be performed. This medium will be used for post-electroporation outgrowth of transformed cells. Maintain the medium at room temperature. 3. Pre-chill electroporation cuvettes and 1.5 ml tubes on ...
problem set
... An operon is a collection of tandemly linked genes that are transcribed in a single polycistronic mRNA. In the E. coli trp operon, the five gene products all work together in the biosynthesis pathway for the amino acid tryptophan. It is advantageous to link genes together in an operon, so that only ...
... An operon is a collection of tandemly linked genes that are transcribed in a single polycistronic mRNA. In the E. coli trp operon, the five gene products all work together in the biosynthesis pathway for the amino acid tryptophan. It is advantageous to link genes together in an operon, so that only ...
After Gel Electrophoresis…
... A technique used in genetic engineering that separates DNA fragments by applying an electric charge to a porous gel ...
... A technique used in genetic engineering that separates DNA fragments by applying an electric charge to a porous gel ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".