Notes_DNA Replication_teacher
... DNA molecule is antiparallel: Complementary strands run in opposite directions. Scientists label the ends 3’ and 5’. Leading and Lagging Strands: DNA polymerase can only attach new nucleotides to the 3’ end of the new DNA strand. This means that it must constantly back track to copy parts of the str ...
... DNA molecule is antiparallel: Complementary strands run in opposite directions. Scientists label the ends 3’ and 5’. Leading and Lagging Strands: DNA polymerase can only attach new nucleotides to the 3’ end of the new DNA strand. This means that it must constantly back track to copy parts of the str ...
Standard Genetic Code
... another when a protein is built. The instructions for building each particular protein is encoded in DNA in the cell nucleus. The instructions are transcribed from DNA into RNA, which then leaves the nucleus and travels to the ribosome where the instructions are used by translating the code into pro ...
... another when a protein is built. The instructions for building each particular protein is encoded in DNA in the cell nucleus. The instructions are transcribed from DNA into RNA, which then leaves the nucleus and travels to the ribosome where the instructions are used by translating the code into pro ...
DNA
... because DNA polymerase must have something to bind to The molecule is replicated in both directions. One side is laid down ...
... because DNA polymerase must have something to bind to The molecule is replicated in both directions. One side is laid down ...
MCQ- V-Semester 2015 - KLE College of Pharmacy
... The first drug produced using recombinant DNA technology was: A) Streptokinase ...
... The first drug produced using recombinant DNA technology was: A) Streptokinase ...
Chapter 12 Notes
... Before a cell divides, it duplicates its DNA in a copying process called _____________________ - This process ensures that each resulting cell has the same complete set of DNA molecules How does the double helix structure of DNA make replication (copying) possible?????? - Each strand of the doub ...
... Before a cell divides, it duplicates its DNA in a copying process called _____________________ - This process ensures that each resulting cell has the same complete set of DNA molecules How does the double helix structure of DNA make replication (copying) possible?????? - Each strand of the doub ...
Transform cells and spread plates
... o Observe the color and glow of the bacteria under a UV light o Fewer colonies of bacteria if amp negatively affected growth o Equal numbers of colonies on both LB nutrient agar and LB/amp agar if amp had no effect o The presence of any colonies on the amp plate would suggest that those bacteria are ...
... o Observe the color and glow of the bacteria under a UV light o Fewer colonies of bacteria if amp negatively affected growth o Equal numbers of colonies on both LB nutrient agar and LB/amp agar if amp had no effect o The presence of any colonies on the amp plate would suggest that those bacteria are ...
Cell Cycle, DNA, and Protein Synthesis
... • The two halves of the doubled structure are called___________________. • Sister chromatids are exact copies of each other and are held together by a __________. • In animal cells, the _______________ move to opposite ends of the cell and start to form _______________ ...
... • The two halves of the doubled structure are called___________________. • Sister chromatids are exact copies of each other and are held together by a __________. • In animal cells, the _______________ move to opposite ends of the cell and start to form _______________ ...
Serial dilution and plate counts
... To calculate the bacterial density in the original suspension follow this calculation: In this example let’s assume that you had 32 colonies on a plate obtained by plating 100µl of a 1x10-6 dilution of the original suspension. First, determine the correction factor to adjust the volume plated on eac ...
... To calculate the bacterial density in the original suspension follow this calculation: In this example let’s assume that you had 32 colonies on a plate obtained by plating 100µl of a 1x10-6 dilution of the original suspension. First, determine the correction factor to adjust the volume plated on eac ...
Document
... C8. These drugs would diminish the amount of negative supercoiling. Negative supercoiling is needed to compact the chromosomal DNA, and it also aids in strand separation. Bacteria might not be able to survive and/or transmit their chromosomes to daughter cells if their DNA was not compacted properly ...
... C8. These drugs would diminish the amount of negative supercoiling. Negative supercoiling is needed to compact the chromosomal DNA, and it also aids in strand separation. Bacteria might not be able to survive and/or transmit their chromosomes to daughter cells if their DNA was not compacted properly ...
learning objectives
... A. The first step of genetic engineering is to cleave the DNA that the geneticist wishes to transfer. B. This process involves the use of restriction enzymes that bind specific sequences of nucleotides and split the DNA in that position. C. Since DNA is made up of complementary bases, both strands d ...
... A. The first step of genetic engineering is to cleave the DNA that the geneticist wishes to transfer. B. This process involves the use of restriction enzymes that bind specific sequences of nucleotides and split the DNA in that position. C. Since DNA is made up of complementary bases, both strands d ...
C1. Self-assembly occurs spontaneously, without the aid of other
... C8. These drugs would diminish the amount of negative supercoiling. Negative supercoiling is needed to compact the chromosomal DNA, and it also aids in strand separation. Bacteria might not be able to survive and/or transmit their chromosomes to daughter cells if their DNA was not compacted properly ...
... C8. These drugs would diminish the amount of negative supercoiling. Negative supercoiling is needed to compact the chromosomal DNA, and it also aids in strand separation. Bacteria might not be able to survive and/or transmit their chromosomes to daughter cells if their DNA was not compacted properly ...
13. DNA Replication
... 1. Review of DNA structure DNA double helix model: DNA made of nucleotide building blocks linked into polymer chains Bases are on inside, sugars and phosphates form a backbone on outside Two strands exist in an antiparallel arrangement ...
... 1. Review of DNA structure DNA double helix model: DNA made of nucleotide building blocks linked into polymer chains Bases are on inside, sugars and phosphates form a backbone on outside Two strands exist in an antiparallel arrangement ...
Manipulating and Analyzing DNA
... change as a species evolves, interspecific analysis of DNA provides a picture of evolutionary relationships between different species. Some of the tools of biotechnology are the natural components of cells. Restriction enzymes are made by bacteria to protect themselves from viruses. They inactivate ...
... change as a species evolves, interspecific analysis of DNA provides a picture of evolutionary relationships between different species. Some of the tools of biotechnology are the natural components of cells. Restriction enzymes are made by bacteria to protect themselves from viruses. They inactivate ...
Ribosomal Protein L11 HDR Plasmid (m): sc-426331
... Target-specific HDR Plasmids provide a DNA repair template for a DSB and, when co-transfected with CRISPR/Cas9 KO Plasmids, enable the insertion of specific selection markers where Cas9-induced DNA cleavage has occurred (1,2). The HDR plasmid can incorporate a Red Fluorescent Protein (RFP) gene to v ...
... Target-specific HDR Plasmids provide a DNA repair template for a DSB and, when co-transfected with CRISPR/Cas9 KO Plasmids, enable the insertion of specific selection markers where Cas9-induced DNA cleavage has occurred (1,2). The HDR plasmid can incorporate a Red Fluorescent Protein (RFP) gene to v ...
The DNA of microorganisms is made up of subunits called A
... microbes in a sample at a specified temperature is called the A. thermal death point (TDP). B. thermal death time (TDT). C. sporicidal time. D. death phase point. E. None of the choices are correct. ...
... microbes in a sample at a specified temperature is called the A. thermal death point (TDP). B. thermal death time (TDT). C. sporicidal time. D. death phase point. E. None of the choices are correct. ...
Fruitful DNA Extraction
... studying DNA molecules). Because DNA mutations accumulate over evolutionary time, the same gene in different individuals or species may be made up of slightly different DNA sequences. This sequence of nucleotide bases is called an organism’s genotype. This molecular data can be used to determine evo ...
... studying DNA molecules). Because DNA mutations accumulate over evolutionary time, the same gene in different individuals or species may be made up of slightly different DNA sequences. This sequence of nucleotide bases is called an organism’s genotype. This molecular data can be used to determine evo ...
Standard 1 answer key. SB1a. Cell theory: states all cells come from
... 27. Vegetative reproduction is a method of asexual reproduction when a piece of the plant is attached back to the parent plant and is used to harvest many plants at a given time. (SB2f) 28. DNA fingerprint is a representation of part of an individual’s DNA that can be used to identify a person at th ...
... 27. Vegetative reproduction is a method of asexual reproduction when a piece of the plant is attached back to the parent plant and is used to harvest many plants at a given time. (SB2f) 28. DNA fingerprint is a representation of part of an individual’s DNA that can be used to identify a person at th ...
Mr Proffitt – IB Biology Name Unit 3 Test Multiple Choice – 1 Mark
... Short Answer – Various marks. Answer, to the best of your ability, the following questions. Be sure to pay attention to the number of marks available for each question! 15. The micrograph below shows an adult human stem cell. ...
... Short Answer – Various marks. Answer, to the best of your ability, the following questions. Be sure to pay attention to the number of marks available for each question! 15. The micrograph below shows an adult human stem cell. ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".