Transformation
... Transformation of E.coli with Plasmid DNA LABEL one microcentrifuge tube with “+DNA” and a second microcentrifuge tube with “-DNA”. TRANSFER 500 µL ice-cold CaCl2 solution into the ”– DNA” tube using a sterile 1 mL pipet. Using a toothpick, TRANSFER approx. 15 well-isolated colonies (each colony sho ...
... Transformation of E.coli with Plasmid DNA LABEL one microcentrifuge tube with “+DNA” and a second microcentrifuge tube with “-DNA”. TRANSFER 500 µL ice-cold CaCl2 solution into the ”– DNA” tube using a sterile 1 mL pipet. Using a toothpick, TRANSFER approx. 15 well-isolated colonies (each colony sho ...
DNA
... bacteria and injected it along with type R bacteria into mice The mice died and their bodies contained active type S bacteria Concluded that DNA passed from type S bacteria to type R, making it lethal ...
... bacteria and injected it along with type R bacteria into mice The mice died and their bodies contained active type S bacteria Concluded that DNA passed from type S bacteria to type R, making it lethal ...
Discovery of a “transforming principle”
... bacteria and injected it along with type R bacteria into mice The mice died and their bodies contained active type S bacteria Concluded that DNA passed from type S bacteria to type R, making it lethal ...
... bacteria and injected it along with type R bacteria into mice The mice died and their bodies contained active type S bacteria Concluded that DNA passed from type S bacteria to type R, making it lethal ...
RODENT GENOTYPING The proper identification of transgenic
... be obtained by a variety of techniques that vary in terms of the discomfort the animal may experience. When animals are marked by ear punches or notches, sufficient tissue can be saved to do the PCR. If the animals are to be marked this way anyway, then this technique adds no additional discomfort t ...
... be obtained by a variety of techniques that vary in terms of the discomfort the animal may experience. When animals are marked by ear punches or notches, sufficient tissue can be saved to do the PCR. If the animals are to be marked this way anyway, then this technique adds no additional discomfort t ...
DNA
... • Exons are sections of coding DNA – i.e. they contain instructions for making proteins. • Introns are sections of non-coding DNA (once called "junk DNA") – i.e. they do not contain instructions for making proteins but are now believed to serve other important ...
... • Exons are sections of coding DNA – i.e. they contain instructions for making proteins. • Introns are sections of non-coding DNA (once called "junk DNA") – i.e. they do not contain instructions for making proteins but are now believed to serve other important ...
Slide 1
... In nature, genes can be transferred between bacteria in three ways: • Conjugation – ...
... In nature, genes can be transferred between bacteria in three ways: • Conjugation – ...
Quick DNA Extraction from Rice Seed (Wet)
... at 500 strokes/minute for two minutes. Centrifuge for 1 min to bring all liquid to the bottom of the assay block. Incubate the samples in about 1” of water at 95ºC for 20 minutes then place them on ice for approximately 10 minutes or until samples are cool to the touch. Centrifuge again for 1 minute ...
... at 500 strokes/minute for two minutes. Centrifuge for 1 min to bring all liquid to the bottom of the assay block. Incubate the samples in about 1” of water at 95ºC for 20 minutes then place them on ice for approximately 10 minutes or until samples are cool to the touch. Centrifuge again for 1 minute ...
Copying DNA: Southern Blotting
... Step 1: Restriction enzymes cut DNA making sticky ends Step 2: These sequences are combined with the DNA of the organism you want to change (recombinant DNA) ...
... Step 1: Restriction enzymes cut DNA making sticky ends Step 2: These sequences are combined with the DNA of the organism you want to change (recombinant DNA) ...
Chapter 4 Cellular Metabolism
... The process of splitting two molecule by adding water is called __hydrolysis__ Control of metabolism: ____enzymes___control the rates of all the metabolic reactions of the cell Enzymes are complex __proteins__that function to lower the activation energy of a reaction so it may begin and proceed more ...
... The process of splitting two molecule by adding water is called __hydrolysis__ Control of metabolism: ____enzymes___control the rates of all the metabolic reactions of the cell Enzymes are complex __proteins__that function to lower the activation energy of a reaction so it may begin and proceed more ...
Genetic Fidelity Testing of Tissue Culture Raised Plants - NCS-TCP
... Ten varieties each of banana and sugarcane, seven varieties of black pepper, twenty three varieties of potato and one variety of vanilla plants were obtained from different authentic sources. DNA was isolated from all these varieties by modified CTAB method. ...
... Ten varieties each of banana and sugarcane, seven varieties of black pepper, twenty three varieties of potato and one variety of vanilla plants were obtained from different authentic sources. DNA was isolated from all these varieties by modified CTAB method. ...
No Slide Title - Fenn Schoolhouse
... Insert the Human Gene into Bacteria to Produce Insulin for Diabetics Produce Artificial Sweeteners ...
... Insert the Human Gene into Bacteria to Produce Insulin for Diabetics Produce Artificial Sweeteners ...
Genetic Engineering - Biology Class With Mrs. Caskey
... http://www.hhmi.org/biointeractive/so matic-cell-nuclear-transfer-animation ...
... http://www.hhmi.org/biointeractive/so matic-cell-nuclear-transfer-animation ...
DNA/RNA
... determines the shape, size and growth of an organism 9 Double stranded helix made up of nucleotides – four bases; Adenine, Thymine, Guanine and Cytosine 9 For encoding genetic information, the central feature of DNA structure is the A-T and G-C base pairing. http://student.ccbcmd.edu/~gkaiser/biotut ...
... determines the shape, size and growth of an organism 9 Double stranded helix made up of nucleotides – four bases; Adenine, Thymine, Guanine and Cytosine 9 For encoding genetic information, the central feature of DNA structure is the A-T and G-C base pairing. http://student.ccbcmd.edu/~gkaiser/biotut ...
Class: AP Bio Unit: Genetics Estimated Date Target Reading
... 11/03/11 Explain the Laws of segregation and independent assortment. (Write two probability questions that require use of the rule of multiplication and two probability questions that require use of the rule of addition.) Describe non-mendelian inheritance and human genetic disorders. ...
... 11/03/11 Explain the Laws of segregation and independent assortment. (Write two probability questions that require use of the rule of multiplication and two probability questions that require use of the rule of addition.) Describe non-mendelian inheritance and human genetic disorders. ...
direct genetic testing
... profile and suspect is quantified by the match probability (Pm) Strength of evidence based on likelihood ratio (LR) LR = C / C ‘Prosecutor’s fallacy’ or ‘fallacy of the transposed conditional’ ‘The probability of the DNA evidence, if it came from the suspect, is 1 in 50 million’ ...
... profile and suspect is quantified by the match probability (Pm) Strength of evidence based on likelihood ratio (LR) LR = C / C ‘Prosecutor’s fallacy’ or ‘fallacy of the transposed conditional’ ‘The probability of the DNA evidence, if it came from the suspect, is 1 in 50 million’ ...
200 THINGS TO KNOW AP Biology TEST
... Eutrophication cultural vs natural Oligotrophic lakes and eutrophic lakes photophosphorylation PGA and PGAL C4 plants outcompete C3 in high sunlight because they store extra Carbon to use when CO2 conc low. CAM plants are desert succulents ( cactus) stomata close during day open at night to avoid de ...
... Eutrophication cultural vs natural Oligotrophic lakes and eutrophic lakes photophosphorylation PGA and PGAL C4 plants outcompete C3 in high sunlight because they store extra Carbon to use when CO2 conc low. CAM plants are desert succulents ( cactus) stomata close during day open at night to avoid de ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".