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Molecular Biology Final Exam (Set A)
Molecular Biology Final Exam (Set A)

... basepairs wherever its sequence allows. Since this internal basepairing relies on self-complementary sequence, the way in which an RNA molecule folds is dependent on its nucleotide base sequence, and thus is different for every RNA. The implications of this are that RNA has a much wider range of thr ...
Lab Techniques
Lab Techniques

... Preparative – Can identify a specific fragment based on size ...
Dr. Escobar
Dr. Escobar

... Washes and Colorimetric Detection ...
Document
Document

... to a single locus and the number of repeats varies then up to two bands would be seen for each sample as shown – the length of each band being related to the number of repeats – each of the samples in Fig. 1 has two alleles and is therefore heterozygous. If only one band was seen the person would be ...
Ch5hybridisationSNPRFLP
Ch5hybridisationSNPRFLP

... Biomedical application ...
Topic: Genetics Aim: Describe some methods that can be used to
Topic: Genetics Aim: Describe some methods that can be used to

... Genetic engineering involves biological and chemical methods to change the arrangement of a gene’s DNA. One method of genetic engineering is gene splicing, or recombinant DNA which involves bringing together genetic material from multiple organisms. Recombinant DNA can be made by inserting a gene fr ...
No Slide Title - Merrillville Community School
No Slide Title - Merrillville Community School

... Formed in the nucleus, it then moves out into the cytoplasm ...
Polymerase Chain Reaction
Polymerase Chain Reaction

... Separation: Double Stranded DNA is denatured by heat into single strands. Short Primers for DNA replication are added to the mixture. DNA polymerase catalyzes the production of complementary new strands. Copying The process is repeated for each new strand created All three steps are carried out in t ...
Cell Line Characterization - Sigma
Cell Line Characterization - Sigma

... 104023GMP.BUK: DNA fingerprinting of cell lines with a single multi-locus probe – an appropriate assay for cell line identity testing This DNA fingerprinting assay uses a single restriction endonuclease (HinfI) with MLP 33.15. In addition to the test article, a positive control standard consisting o ...
FlyCutTM XmaI - AP
FlyCutTM XmaI - AP

... enzyme, and then ligated, transformed and plated on X-gal/IPTG plate. Successful expression of the β-galactosidase indicates that lacZα gene remains integrity after cloning. A blue colony represents an intact gene, and a white colony represents an interrupted gene. To be Blue/White certified, enzyme ...
Characterization of Genes Expressed During the Early Stages of
Characterization of Genes Expressed During the Early Stages of

Document
Document

... The base pair in the 3rd codon position can “wobble” ...
Prescott`s Microbiology, 9th Edition 42 Biotechnology and Industrial
Prescott`s Microbiology, 9th Edition 42 Biotechnology and Industrial

... populations of engineered RNA molecules (aptamers) for use in therapies e. Selecting the best variants is more rapid using high-throughput screening (HTS) methods often using robotic 96-well plate assays 6. Metagenomics—since most environmental microbes do not grow in the laboratory, bioprospecting ...
Biotechnology - (www.ramsey.k12.nj.us).
Biotechnology - (www.ramsey.k12.nj.us).

... - chemical which causes cells to have extra set of chromosomes - fruits, flowers, and plants larger - *some cases such as plants and lower animals natural occurrence - lethal for human zygote ...
Cloze passage 4
Cloze passage 4

... r) Environmental factors that induce mutations are known as ……………….. s) A type of mutation where some of the DNA is lost from the chromosome t) 2 English scientists who discovered the structure for DNA u) A female scientist whom the above scientists stole and used her ideas v) The main technique use ...
Agarose Gel Electrophoresis - Cal State LA
Agarose Gel Electrophoresis - Cal State LA

... fragments of similar, but different, lengths Resolution is affected by  agarose ...
Name Date__________________ DNA and Protein Synthesis
Name Date__________________ DNA and Protein Synthesis

... 3. If a DNA sequence consists of 12 nucleotides, how many mRNA codons will there be? 4. The enzyme that creates mRNA from a DNA sequence is called: 5. Each codon of mRNA (hence each triplet in DNA) codes for one: 6. The specific amino acid carried by a tRNA is determined it's: 7. True or False? When ...
dna
dna

... strand Restriction enzymes-Chemicals that cut DNA into fragments that can later be incorporated into another DNA strand; about 150 are commercially available Probe- A single strand of nucleic acid, much like RNA, that has been made in a way that its base sequence lines up to hybridize areas in an al ...
Abstract Dead plant biomass is a key pool of carbon in terrestrial
Abstract Dead plant biomass is a key pool of carbon in terrestrial

... significantly. Results also confirmed that Actinobacteria thrive better than other bacteria in contaminated soils and may thus serve as important degraders of lignocellulose. Finally, to get the most accurate estimates of the relative abundance of fungal and bacterial taxa obtained by NGS, methods ...
Document
Document

... cancer or other illness by looking at cells in the microscope and describing how they appear. This is sometimes not very precise. Microarrays have been used to compare normal and disease tissue for differences in gene expression. This method is very precise and discriminating. Some types of tumors t ...
Slide 1
Slide 1

... When DNA is replicated (doubled) in a cell, you make another DNA helix In mitosis both helices attach to each other at the centromere forming sister chromatids….which makes the X-shaped chromosome you normally think of when ...
Examination in Gene Technology, TFKE38 2011-10-18
Examination in Gene Technology, TFKE38 2011-10-18

... 4) You want to study the ribosomal proteins and has therefore chosen to clone protein L21. The figure below (figure 2) shows the DNA sequence and protein sequence of L21. NOTE: This question provides a total of 20 points a) Construct primers to find the gene in a cDNA library (2p) b) In order to qu ...
Glossary of Biotechnology Terms
Glossary of Biotechnology Terms

... by reverse transcription. A cDNA is so-called because its sequence is the complement of the original mRNA sequence. However, when double-stranded cDNA is synthesized, it contains both the original sequence and its complement. cDNA library: a collection of cDNA's, each of which has been inserted in a ...
Transkriptom a proteom - Univerzita Karlova v Praze
Transkriptom a proteom - Univerzita Karlova v Praze

... Mobile phase = labeled probe of certain gene (signal = presence of certain transcript + info about the transcript size) ...
Polymerase Chain Reaction
Polymerase Chain Reaction

... Amplifying the DNA from a single bacterium or virus using PCR can provide a speedy and accurate diagnosis for serious infections, where getting the right treatment quickly can mean the difference between life and death. PCR is already used in the diagnosis of AIDS, viral meningitis, TB and an ever-g ...
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