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... Evaluate methods used to collect environmental data Consider the validity of the method used to collect the data, and the reproducibility fo the data Relate sample size to both validity and reproducibility Recognise and terms ‘mean’ ‘median’ and ‘mode’ ...
Journal of Bacteriology
Journal of Bacteriology

... in protection responses. Among these are three genes encoding proteins involved in resistance to acriflavine (ngr226, ngr288, and ngr289), genes involved in detoxification of other small molecules (e.g., ngr065, ngr334, two copies of the multidrug resistance protein B), CopC (copper resistance prote ...
The Role of real-time PCR in the CBRN Environment Palaiodimos L
The Role of real-time PCR in the CBRN Environment Palaiodimos L

... Joint CBRN Defense Company, Hellenic National Defense General Staff The rapid detection of Biological Warfare Agents (BWAs) is crucial in order to proceed with: • The necessary measures to eliminate the ...
chapter 17 from gene to protein
chapter 17 from gene to protein

...  This establishes the reading frame; subsequent codons are read in groups of three nucleotides.  The cell’s protein-synthesizing machinery reads the message as a series of nonoverlapping three-letter words.  In summary, genetic information is encoded as a sequence of nonoverlapping base triplets, ...
Protists have divergent effects on bacterial diversity along a
Protists have divergent effects on bacterial diversity along a

... Zubkov et al. (2007). Bacterial diversity was assessed using terminal restriction fragment length polymorphism (tRFLP) where amplified bacterial DNA is cut using a site-specific endonuclease, and the sizes of the resulting restriction fragments are measured. Our tRFLP protocol is described by Thomso ...
Localized hypermutation and associated gene losses in legume
Localized hypermutation and associated gene losses in legume

High performance solution-based target selection using individually
High performance solution-based target selection using individually

... The availability of solution-based genomic target selection techniques has enabled rapid development of targeted sequencing applications, some of which have led to the introduction of clinical sequencing tests. Commercialized hybridization capture reagents are based on array-synthesized oligonucleot ...
Chapter 6: Microbial Nutrition and Growth
Chapter 6: Microbial Nutrition and Growth

... atmospheric oxygen must produce an enzyme superoxide dismutase (SOD), to get rid of them. SOD is made by aerobes, facultative anaerobes, and aerotolerant anaerobes, but not by anaerobes or microaerophiles. ...
Molecular Plant Microbe Interactions
Molecular Plant Microbe Interactions

... Fig. 1. Growth dependence of CFNEA31 mutant on genistein in the presence of kanamycin. Growth of CFNEA31, carrying the nptII kanamycin resistance gene as a promoter probe from Tn5::B30, was screened by testing kanamycin (100 µg/ml) resistance with genistein 1 µM (n), 5 µM (▲), or without genistein ( ...
Programa i llibre de resums - Societat Catalana de Biologia
Programa i llibre de resums - Societat Catalana de Biologia

... also for irrigation of fresh produce for human consumption. Water quality, is evaluated according to FIB levels (Fecal Indicator Bacteria), but these indicators do not always correlate with enteric viruses. Wastewater contains many potential well known pathogenic bacteria and viruses; but also other ...
See more scientific discussion on this here.
See more scientific discussion on this here.

... effect. It was not selected against by Natural Selection. What other explanation could there be? It is also a definite piece of evidence for evolution in the sense just described but also because this non-functional gene has the same missing parts in all of the anthropoid primates. ...
Genome Transplantation in Bacteria: Changing One Species to
Genome Transplantation in Bacteria: Changing One Species to

... Genome Transplantation into M. capricolum •  Prepared M. mycoides genome was transplanted into nucleotide-starved M. capricolum cells, at low efficiencies •  Blue colonies (M. mycoides) formed after 3 days •  Smaller blue and white colonies (M. capricolum) formed after 10 days ▫  Result of recombina ...
PDF
PDF

... right end of the sub-sequence. Thus, it is possible to search for the closest B site to the left of the first A site without regard to how many other B sites occur between LEND and A. An example of a useful search specification involves the restriction site, although more complex search arguments ca ...
11. Appendix A – PCR Reagent Concentration and Master Mix Tools
11. Appendix A – PCR Reagent Concentration and Master Mix Tools

GPR17 shRNA Plasmid (r): sc-270023-SH
GPR17 shRNA Plasmid (r): sc-270023-SH

... concentrations of nucleotides and cysteinyl leukotrienes (CysLTs), two families of endogenous signaling molecules, increase significantly at the site of damage. In some neurons, GPR17, a membrane receptor for uracil nucleotide and CysLTs, is upregulated as well, infiltrating the lesioned area. GPR17 ...
FEMS Microbiology Letters
FEMS Microbiology Letters

... brasilense Sp7. After hydrolysis of the purified protein, amino acid sequences of five peptides were obtained, which permitted the cloning and sequencing of the cysK gene. The deduced amino acid sequence of cysteine synthase exhibited homology with several putative proteins from Alpha- and Gammaprot ...
Towards biome-specific analysis of meta-omics data
Towards biome-specific analysis of meta-omics data

... metabolic processes in the gut. The color scale reflects significantly enriched abundances in Healthy compared to Crohn’s Disease (CD) subjects. (b) Chord plot highlighting species-function associations. Modules (MF numbers) belonging to the same global metabolic process share the same color. Associ ...
5. Biodiversity
5. Biodiversity

... prevent environmental degradation” ...
Why don’t antibodies get rid of HIV?
Why don’t antibodies get rid of HIV?

... • DNA replication ensures that genetic information is passed on unchanged from a cell to its descendents. • The major thing cells do with genetic information is use it to encode PROTEINS. • Every cell contains all of an organism’s genes, so each cell could (in theory) make every protein. But which p ...
Metoda Pemuliaan Tanaman Secara Khusus
Metoda Pemuliaan Tanaman Secara Khusus

... Increases in yield are derived both from improved varieties and from improved management. It suggests about a 50-50 of crop yield split between genetic gain and gain attributed to management. Planting material is the most important input in agriculture An improved cultivar is the most economic a ...
PDF
PDF

... from mature adult Ciona intestinalis animal (data not shown). The full length sequence of the cDNA clone was obtained by using the GeneRacerTM kit (Invitrogen, USA). The kit ensures the amplification of only full length transcript via elimination of truncated messages from the amplification process. ...
DNA purification and isolation of genomic DNA from bacterial
DNA purification and isolation of genomic DNA from bacterial

... microgram amounts of DNA, are still performed in molecular biology laboratories, most assessment of chromosomal DNA is done by PCR technology including monoplex or multiplex PCR, SNP analysis and real-time PCR. These latter techniques use nanogram amounts of DNA per reaction. Regardless of the syste ...
Full-Text PDF
Full-Text PDF

... outbreaks of several pathogen microorganisms. Time-analysis saving methods able to rapidly identify the pathogen microorganism causing an outbreak is of great importance for this kind of monitoring and electrochemical multiplexed sensor systems, which would also be sensitive, accurate, simpler and c ...
1 Lecture 5 Molecular biology Dr. Israa Al
1 Lecture 5 Molecular biology Dr. Israa Al

DNA - Napa Valley College
DNA - Napa Valley College

... Watson and Crick put all the pieces of information together. They built models to help them come up with the structure. They knew it was a race so they published a one page article in Nature (1953) with their ideas – they performed no experiments but were able to see the big picture Crick, Watson a ...
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