A Mutation Causing Reduced Biological Activity and Stability of
... mature secreted protein does not alter its biological or immunological properties (16,17). Since carbohydrate moieties are often required for the proteolytic or conformational stability of the protein component of glycoproteins (18), we searched for other examples of naturally occurring mutations re ...
... mature secreted protein does not alter its biological or immunological properties (16,17). Since carbohydrate moieties are often required for the proteolytic or conformational stability of the protein component of glycoproteins (18), we searched for other examples of naturally occurring mutations re ...
DNA and the Genome - Speyside High School
... RNA splicing After the mRNA has been transcribed the introns are removed. The remaining exons are spliced together to form a continuous sequence. This is called the mature transcript. The mature transcript then leaves the nucleus to travel to the cytoplasm. CFE Higher Biology ...
... RNA splicing After the mRNA has been transcribed the introns are removed. The remaining exons are spliced together to form a continuous sequence. This is called the mature transcript. The mature transcript then leaves the nucleus to travel to the cytoplasm. CFE Higher Biology ...
Basics of sequence analysis Ch.6 and Ch.7
... Should result of alignment include all amino acids or proteins or just those that "match"? If yes, a global alignment is desired In a global alignment, presence of mismatched elements is neutral - doesn't affect overall match score Should result of alignment include all amino acids or proteins or j ...
... Should result of alignment include all amino acids or proteins or just those that "match"? If yes, a global alignment is desired In a global alignment, presence of mismatched elements is neutral - doesn't affect overall match score Should result of alignment include all amino acids or proteins or j ...
Microbial Growth Lecture PowerPoint
... Images: Helicobacter pylori, Y. Tsutsumi, M.D., Fujita Health University School of Medicine; pH scale, Edward Stevens ...
... Images: Helicobacter pylori, Y. Tsutsumi, M.D., Fujita Health University School of Medicine; pH scale, Edward Stevens ...
Method and system for computationally identifying clusters within a
... symbols or the one-letter symbols, listed in Table 1, for the amino acids of the protein, starting from the N-terminal amino acid on the left side and ending With the C-terminal amino acid on the right side. For example, the polypeptide polymer displayed in FIG. 2 can be described either as “ALA-TYR ...
... symbols or the one-letter symbols, listed in Table 1, for the amino acids of the protein, starting from the N-terminal amino acid on the left side and ending With the C-terminal amino acid on the right side. For example, the polypeptide polymer displayed in FIG. 2 can be described either as “ALA-TYR ...
Determination of the Binding Site-Size of the Protein
... Step 2. Preparation of protein-DNA complexes Binding mode is not always the same for several DNA-binding proteins. For example, differences in the binding condition, such as the ionic strength, pH and some small molecules included in the reaction mixture, can affect the binding mode of SSB (8). Thes ...
... Step 2. Preparation of protein-DNA complexes Binding mode is not always the same for several DNA-binding proteins. For example, differences in the binding condition, such as the ionic strength, pH and some small molecules included in the reaction mixture, can affect the binding mode of SSB (8). Thes ...
AI for Synthetic Biology
... • Parts used as basis for engineering • Fluorescent proteins can be observed and used to help understand what is going on in a cell ...
... • Parts used as basis for engineering • Fluorescent proteins can be observed and used to help understand what is going on in a cell ...
Figure 1000G Allele Frequencies
... a. Alternative translation start side: If the lost-of-function variant is near the beginning of the protein, translation could be initiated by a downstream in-frame AUG [30]. In our study, if the indel is in the first 25 codons (first 75 bp of translated cDNA) or within 5th percentile of the coding ...
... a. Alternative translation start side: If the lost-of-function variant is near the beginning of the protein, translation could be initiated by a downstream in-frame AUG [30]. In our study, if the indel is in the first 25 codons (first 75 bp of translated cDNA) or within 5th percentile of the coding ...
MB206_fhs_lnt_001.1_AT_May09
... among different bacteria. DNA is supercoiled and tightly packaged. The extent of sequence homology between DNAs from different microorganisms determines how closely related they are (eg. 16sRNA sequence) ...
... among different bacteria. DNA is supercoiled and tightly packaged. The extent of sequence homology between DNAs from different microorganisms determines how closely related they are (eg. 16sRNA sequence) ...
Why measure bacterial production?
... about what we mean by growth efficiency. If the measure of growth is the change in bacterial numbers in a “predator-free” fraction, one must be certain that the fraction is indeed predator-free. How should we conceptualize bacterial mortality due to phage or bacteria within this fraction? Obviously ...
... about what we mean by growth efficiency. If the measure of growth is the change in bacterial numbers in a “predator-free” fraction, one must be certain that the fraction is indeed predator-free. How should we conceptualize bacterial mortality due to phage or bacteria within this fraction? Obviously ...
Document
... database. This database will be useful for researchers working on polysaccharide-based vaccines. It is freely available from the URL: http://crdd.osdd.net/raghava/polysacdb/. Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Anti ...
... database. This database will be useful for researchers working on polysaccharide-based vaccines. It is freely available from the URL: http://crdd.osdd.net/raghava/polysacdb/. Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Anti ...
Study of the arginine repressor in different organisms
... for solvent content is 55.8 %. Crystals of the repressor in the presence of its corepressor arginine in 30% PEG400, 0.2 M CaCl2, 0.1 M Na-HEPES pH 7.5 appeared faster than those for the aporepressor but were smaller. A data set to a resolution of 2.4 Å was collected from a single crystal. The crysta ...
... for solvent content is 55.8 %. Crystals of the repressor in the presence of its corepressor arginine in 30% PEG400, 0.2 M CaCl2, 0.1 M Na-HEPES pH 7.5 appeared faster than those for the aporepressor but were smaller. A data set to a resolution of 2.4 Å was collected from a single crystal. The crysta ...
Properties and sequence of the coenzyme B12
... pLM3 was used to transform E. coli K38/pGP1-2, which contains on the plasmid pGP1-2 bacteriophage T7 RNA polymerase under control of the VpL promoter and the temperature-sensitive cI857 V repressor. Expression of the genes was induced by a shift in temperature from 30 to 42³C [10]. Induction of tran ...
... pLM3 was used to transform E. coli K38/pGP1-2, which contains on the plasmid pGP1-2 bacteriophage T7 RNA polymerase under control of the VpL promoter and the temperature-sensitive cI857 V repressor. Expression of the genes was induced by a shift in temperature from 30 to 42³C [10]. Induction of tran ...
Comparative genomics of regulation of heavy
... spacer (the 7-4-7 configuration, consensus TCCGTAC(4)-GTACGGA). The promoter spacer length is a crucial feature of a normally functioning site. The MerR signal of firmicutes is a 9-4-9 palindrome (with consensus ACCGTGTAC-(4)-GTACAGGGT) in a 20-bp promoter spacer (See Additional file 1, sheet2 "merR ...
... spacer (the 7-4-7 configuration, consensus TCCGTAC(4)-GTACGGA). The promoter spacer length is a crucial feature of a normally functioning site. The MerR signal of firmicutes is a 9-4-9 palindrome (with consensus ACCGTGTAC-(4)-GTACAGGGT) in a 20-bp promoter spacer (See Additional file 1, sheet2 "merR ...
Effect of Six Decades of Selective Breeding on
... of certain amino acids such as methionine, cysteine, threonine, tryptophan, isoleucine, valine, and arginine would obviate the use of expensive synthetics necessary to build complete rations for growing swine and poultry (32, 33). To determine whether changes in the relative content of amino acids h ...
... of certain amino acids such as methionine, cysteine, threonine, tryptophan, isoleucine, valine, and arginine would obviate the use of expensive synthetics necessary to build complete rations for growing swine and poultry (32, 33). To determine whether changes in the relative content of amino acids h ...
Title
... allow for the dual nature of most parameters (low values are as important as high values). To each variable X was thus associated a twin X’ varying in an opposite sense (X’= 40-X). Such a doubling proved useful when dealing with ecological gradients (Ponge et al. 1997) or when it ...
... allow for the dual nature of most parameters (low values are as important as high values). To each variable X was thus associated a twin X’ varying in an opposite sense (X’= 40-X). Such a doubling proved useful when dealing with ecological gradients (Ponge et al. 1997) or when it ...
Slide 1
... formation of cystine calculi in the kidneys due to low solubility of cystine in acidic environment. Clinically, cystinuria is divided into two types: • Type I cystinuria – heterozygotes have normal excretion of cystine and dibasic amino acids, which implies that the disease is inherited autosomal-re ...
... formation of cystine calculi in the kidneys due to low solubility of cystine in acidic environment. Clinically, cystinuria is divided into two types: • Type I cystinuria – heterozygotes have normal excretion of cystine and dibasic amino acids, which implies that the disease is inherited autosomal-re ...
Use of molecular markers and major genes in the genetic
... variation and major genes for the genetic improvement of livestock. Information concerning the basis of these techniques and their application to the genetic improvement of animals is reviewed. Main marker molecular marker systems in animals (RFPL and microsatellites), genome maps, methods for detec ...
... variation and major genes for the genetic improvement of livestock. Information concerning the basis of these techniques and their application to the genetic improvement of animals is reviewed. Main marker molecular marker systems in animals (RFPL and microsatellites), genome maps, methods for detec ...
Molecular and Structural Characterization of
... glucosidase genes for heterologous expression in Escherichia coli and examined the SDS-PAGE profiles of the respective recombinant proteins. The wheat glucosidases corresponding to the mature enzymes were overexpressed with and without an N-terminal His-tag in the BL21 CodonPlus(DE3)-RIL strain. Whi ...
... glucosidase genes for heterologous expression in Escherichia coli and examined the SDS-PAGE profiles of the respective recombinant proteins. The wheat glucosidases corresponding to the mature enzymes were overexpressed with and without an N-terminal His-tag in the BL21 CodonPlus(DE3)-RIL strain. Whi ...
Part 1
... an N-containing medium. Throughout the period of growth, samples were taken, cells lysed and the DNA analyzed by centrifugation in CsCl gradient. The parent DNA showed 1 band in CsCl gradient corresponding to 15N DNA, the 1st generation daughter molecules also showed 1 band which was not at the same ...
... an N-containing medium. Throughout the period of growth, samples were taken, cells lysed and the DNA analyzed by centrifugation in CsCl gradient. The parent DNA showed 1 band in CsCl gradient corresponding to 15N DNA, the 1st generation daughter molecules also showed 1 band which was not at the same ...
Review article - HAL
... heterogeneity. Therefore, an abnormal test result does not diagnose MFS, and a normal test result does not rule out MFS. The identification of the FBN1 gene has allowed the development of two types of diagnostic tests: either genetic family studies or mutation identification. Family studies can be p ...
... heterogeneity. Therefore, an abnormal test result does not diagnose MFS, and a normal test result does not rule out MFS. The identification of the FBN1 gene has allowed the development of two types of diagnostic tests: either genetic family studies or mutation identification. Family studies can be p ...