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The Microbial World:
The Microbial World:

... microorganisms (fungi and protists). It is now also known that many more forms of microbial life exist on Earth than previously expected. Indeed, most of the organisms from natural soil and aquatic communities have not yet been grown in culture and characterized. Thus, in contrast to plants and vert ...
Isolation of a non-genomic origin fluoroquinolone
Isolation of a non-genomic origin fluoroquinolone

... and ␤-galactosidase have led to successful isolation of constitutive promoters of varying strengths (8,17–19). As compared to directed evolution of native promoters, combinatorial strategy poses challenge of managing large diversity of clones. In an attempt to isolate both inducible and constitutive ...
Slide 1
Slide 1

... binding of a repressor to the operator shuts off transcription  The trp operon is a repressible operon  An inducible operon is one that is usually off; a molecule called an inducer inactivates the repressor and turns on transcription ...
(BACTERIOCIN-LIKE INHIBITORY SUBSTANCES) PRODUCED BY
(BACTERIOCIN-LIKE INHIBITORY SUBSTANCES) PRODUCED BY

... alternative agents either in food biopreservation or in prevention/treatment of bacterial infections due to their antimicrobial action against human, plant and animal pathogens, showing potential biotechnological applications. Bacteriocin-like inhibitory substances (BLIS) are antimicrobial agents si ...
1. Translation
1. Translation

... An additional control system is superimposed on the repressor operator system. This control system is thought to have evolved because the cell can capture more energy from the breakdown of glucose than it can from the breakdown of other sugars. If both lactose and glucose are present, the synthesis ...
Microbial Source Tracking - Rutgers Cooperative Extension Water
Microbial Source Tracking - Rutgers Cooperative Extension Water

... When a fecal source database is constructed it is “internally tested.” Each microbial isolate in the fecal source database is treated as an “unknown” and compared to all of the other isolates in the database. The statistical pattern-matching algorithm (see below) “classifies” that isolate as to its ...
Genetic Testing for Mitochondrial Disorders
Genetic Testing for Mitochondrial Disorders

... Genetic test results can be complex. It is important that your physician and/or genetic counselor discuss your test results with you based on the type of test performed and in the context of your medical and family history. Possible results include: • A positive result indicates that a previously u ...
Complete Elimination of Endosymbiotic Algae from Paramecium
Complete Elimination of Endosymbiotic Algae from Paramecium

... single species or not. We have previously cloned endosymbiotic algae from P. bursaria and characterization of each clone was carried out (Nishihara et al. 1998). On the other hand, we have reported a new technique to produce algae-free paramecia by treating green paramecia with a herbicide, paraquat ...
Gene cloning tutorial
Gene cloning tutorial

... technology. You are provided with a series of cards. These begin with a general introduction (cards 2-3) and the outline of the characteristics of the three particular proteins (cards 4-6). After choosing which protein you want to produce you should work through the remaining cards and produce a str ...
Active site amino acid sequence of the bovine O6
Active site amino acid sequence of the bovine O6

... membrane. Total yield was about 800 pmole active enzyme (0.5% recovery) with a specific activity of 300 units/mg protein (500-fold purification). Incubation of the enzyme with [3H]-labelled substrate followed by SDS-Page electrophoresis and fluorography showed a major radioactive product of about 24 ...
Antimicrobial resistance: Mechanisms of action of antimicrobial agents
Antimicrobial resistance: Mechanisms of action of antimicrobial agents

ESCHERICHIA COLI
ESCHERICHIA COLI

... to interspecies [13]. Humans use secondary metabolites in the form of herbal and recreational drugs as well as medicines. In recent years, the use of some secondary metabolites as an alternative to conventional antibiotics has generated the interest in human health research. More than 13,000 seconda ...
Millport Plankton
Millport Plankton

... important for us to understand these interactions and improve our knowledge about these small uni- and multi-celluar organisms, which the majority of our ocean life depends on. This matter is even more urgent as anthropogenic activities continue to disrupt natural biogeochemical cycles and in the la ...
S. marcescens - York College of Pennsylvania
S. marcescens - York College of Pennsylvania

... Klebsiella pneumoniae and Serratia marcescens are among the most opportunistic pathogens and frequently encountered gram-negative organisms in nosocomial infections. Recent work has shown that gram-negative bacteria release membrane vesicles (MV), which contain proteins, lipopolysaccharides, phospho ...
E. coli
E. coli

... Factor IX is a 415 amino acid plasma glycoprotein which has an essential role in blood clotting. Production of the protein is vital for the treatment of haemophilia. Factor IX is synthesised in liver hepatocytes where it undergoes three distinct types of post-translational modification. These modifi ...
knockdown
knockdown

... query and database If there is a matching word W, performs an ungapped alignment between the query and database sequence, extending the match in each direction High-scoring matches then subjected to a gapped alignment between the query sequence and the database sequence using a variation of the Smit ...
Document
Document

... • These noncoding regions are called intervening sequences, or ____________ • The other regions are called ____________ because they are eventually expressed, usually translated into amino acid sequences • _____________________removes introns and joins exons, creating an mRNA molecule with a ...
The Complete Genome Sequence of Clostridium aceticum: a
The Complete Genome Sequence of Clostridium aceticum: a

... Anaerobic sludge was used for those experiments, so that no single organism could be identified that was responsible for this metabolic activity. The first pure culture of such a bacterium was isolated by Wieringa (2), who later described it as Clostridium aceticum (3, 4). This is an anaerobic, endo ...
B333Syllabus - Home
B333Syllabus - Home

... 2. Laboratory. The microbiology laboratory is an important part of the course. Foremost, it aims to introduce you to the basic skills and techniques used to identify bacteria of medical importance. Simultaneously, it allows you to 'get to know' bacteria, appreciate their morphological and metabolic ...
determination of tantalum in high-tantalum ferro-alloys
determination of tantalum in high-tantalum ferro-alloys

... this dilution. From the graph it was possible to calculate the specific activity which would have been obtained in the absence of self-shielding, and this was shown to be greater than that obtained from a single grain of tantalum pentoxide. When a solution of tantalum, of such dilution that the self ...
Quantification and Classification1 - IC
Quantification and Classification1 - IC

... if, for example, in a well-supported tree, a bacterial protein groups with its eukaryotic homologs to the exclusion of homologs from other bacteria and, best of all, shows a reliable affinity with a particular eukaryotic lineage, the conclusion that horizontal gene transfer is at play seems inevitab ...
PowerPoint
PowerPoint

... the physical appearance of an organism. In some cases, undesirable traits may develop along with those that are desired. ...
Document
Document

... advent of Southern transfer and the associated hybridization techniques made it possible for the first time to obtain information about the physical organization of single and multicopy sequences in complex genomes. The term Southern blotting, now used to describe any type of DNA transfer from gel t ...
Integrated Microfluidic Nucleic Acid Isolation, Isothermal
Integrated Microfluidic Nucleic Acid Isolation, Isothermal

... phosphate or ATP concentration, some of which can be monitored by luminescence reactions using, e.g., luciferase/luciferin. Generally, this NAAT laboratory protocol requires six or more hours, and is carried out by skilled technicians in dedicated facilities equipped with hoods, centrifuges, tempera ...
Document
Document

... (and as the data and biology allow) For example, the nature and rate of change can PAPER 36 be allowed to differ at different positions along the molecule, from one branch of the tree to another, through time, etc. Sites can be considered to be interdependent. © Mark Ragan 2004 ...
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