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Unit 8 - Macromolecules Processes
Unit 8 - Macromolecules Processes

... U8 - Macromolecule Processes Notes ...
DNA/RNA
DNA/RNA

Keynote for 2008 Genomics Workshop
Keynote for 2008 Genomics Workshop

... but most are crop plants. If we count only medicinal plants, generously defined to include makers of secondary metabolites with purported health benefits, such as lycopene for tomatoes and resveratrol for grapes, there are 16 plant species with more than 20,000 ESTs. If we use a strict definition of ...
ppt - Faculty
ppt - Faculty

... DNA replication involves a great many building blocks, enzymes and a great deal of ATP energy. DNA replication in humans occurs at a rate of 50 nucleotides per second and ~500/second in prokaryotes. Nucleotides have to be assembled and available in the nucleus, along with energy to make bonds betwe ...
Biology 303 EXAM II 3/14/00 NAME
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Forensics of DNA

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Chromosome Contact Matrices
Chromosome Contact Matrices

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Lecture 2 Nucleic Acid Structure

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... Shotgun-sequencing Method First proposed by Craig Venter, Hamilton Smith and Leroy Hood in 1996, focuses on the sequencing stage and then mapping., it starts with a BAC clone with very large inserts, averaging about 150 kb. The inserts in each BACs are sequenced on both ends using an automated seque ...
ppt - Department of Computer Science
ppt - Department of Computer Science

... in a dense form where it cannot be transcribed.  To begin transcription requires a promoter, a small specific sequence of DNA to which polymerase can bind (~40 base pairs “upstream” of gene)  Finding these promoter regions is a partially solved problem that is related to motif finding.  There can ...
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SG 17,18,19

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Nucleotide-Sugar Transporters in Plants

Test Answers - WordPress.com
Test Answers - WordPress.com

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... Karyotype (know how to read and analyze): Be able to analyze blood types as co-dominant and multiple alleles Be able to set up and complete a dihybrid Punnett square and analyze probability ratios for genotype and phenotype. Homologous/Non-homologous chromosomes Mutations of gametes: Deletion, inver ...
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... - reactive oxygen species (hydrogen peroxide, hydroxide radicals); common product is oxoG UV light causes pyrimidine dimers, such as thymine dimers Ionizing radiation (x rays, gamma rays) cause ds DNA breaks Bleomycin (anti cancer drug) causes ds breaks Base analogs – what are they? A common example ...
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AP genetic technology

... • DNA from areas with tandem repeats is cut with restriction enzymes • Because of the variation in the amount of repeated DNA, the restriction fragments vary in size • Variation is detected by gel electrophoresis ...
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DNA Timeline - WordPress.com

... • Awarded the 1958 Nobel Prize for Physiology or Medicine ...
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genetics, dna replication, protein synthesis, biotechnology

... 3. Curly hair in humans, white fur in guinea pigs, and needle like spines in cacti all partly describe each organisms a. Alleles b. Autosomes c. Chromosomes d. Phenotype 4. The appearance of a recessive trait in offspring of animals most probably indicates that a. Both parents carried at least one r ...
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5 Conclusion - Duke Computer Science

... appear similar to the classical "one-time pad" cryptographic technique, it is in fact reusable, and is thus a more generally useful procedure than the one-time pad technique, because the same primer sequences (and encryption key) could be employed on many separate occasions for communications betwee ...
C16 DNA
C16 DNA

... DNA ligase – enzyme that joins the Okazaki fragments into a single DNA strand. Primer – short stretch of RNA (~10 nucleotides long) that serves as the template. Only one primer is needed for a DNA polymerase to begin synthesizing the leading strand. For the lagging strand, each fragment must be prim ...
Slide 1
Slide 1

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... • ↓ mRNA and protein levels of P11 in the PFC of FSL compared to FRL • P11 mRNA between naive FSL & FRL not statistically significant with qRT-PCR – ↑ cellular resolution with in-situ hybridization ...
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Tool 1

... size using electrical current. The protocols in current use involve using capillary electrophoresis for this. The different DNA fragments are distinguished within the same lane gel by their colour coding which are read using a laser. MLVA has an important disadvantage: it is (at least up till now) n ...
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Bisulfite sequencing



Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).
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