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Chapter 12 Test Review
Chapter 12 Test Review

... 20. During transcription, the hydrogen bonds between base pairs are broken. 21. A three-base code (on mRNA) for an amino acid is called a codon. 22. This type of RNA, along with proteins, makes up the structure of a ribosome rRNA. 23. Which organelle makes proteins when its rRNA moves along the mRNA ...
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TRANSCRIPTION TO TRANSLATION
TRANSCRIPTION TO TRANSLATION

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Bisulfite sequencing



Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).
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