Lecture 6 Gene expression: microarray and deep sequencing
... Within every probeset, each probe has different sensitivity/specificity, caused by cross-hybridization, different chemical properties etc. Across chips, the fluorescence intensity-concentration response curve can be different, caused by variations in sample processing, image reader etc. ...
... Within every probeset, each probe has different sensitivity/specificity, caused by cross-hybridization, different chemical properties etc. Across chips, the fluorescence intensity-concentration response curve can be different, caused by variations in sample processing, image reader etc. ...
AP Biology Genes Review Questions Experiments by Avery
... 1. Experiments by Avery, MacLeod, and McCarty supported DNA as the genetic material by showing that a. Both protein and DNA samples provided the transforming factor. b. DNA was not complex enough to be the genetic material c. Only samples with DNA provided transforming activity d. Even though DNA wa ...
... 1. Experiments by Avery, MacLeod, and McCarty supported DNA as the genetic material by showing that a. Both protein and DNA samples provided the transforming factor. b. DNA was not complex enough to be the genetic material c. Only samples with DNA provided transforming activity d. Even though DNA wa ...
DNA Structure and Function
... • Next replication includes these changes • Source of new alleles (new variation) – A few are beneficial (make a better protein) – Most are silent (make no change in a protein) – Some are harmful (most that change a ...
... • Next replication includes these changes • Source of new alleles (new variation) – A few are beneficial (make a better protein) – Most are silent (make no change in a protein) – Some are harmful (most that change a ...
BIG IDEA #2 - Science - Miami
... Meiosis: Making Haploid Cells Used for Sexual Reproduction Introduction: Genes, Mutations and Viruses ...
... Meiosis: Making Haploid Cells Used for Sexual Reproduction Introduction: Genes, Mutations and Viruses ...
Detection of Viral, Bacterial and Human Genomic DNA
... denaturation, 50 cycles of 95°C for 15 seconds for denaturation and 60°C for 30 seconds for annealing and extension. For the detection of bacterial DNA, 2 L of isolated DNA was added to 18 L of real-time PCR reaction mixture (SYBR Green) containing 1 μM 16S primer mix. SYBR Green reactions were ru ...
... denaturation, 50 cycles of 95°C for 15 seconds for denaturation and 60°C for 30 seconds for annealing and extension. For the detection of bacterial DNA, 2 L of isolated DNA was added to 18 L of real-time PCR reaction mixture (SYBR Green) containing 1 μM 16S primer mix. SYBR Green reactions were ru ...
MITOCHONDIAL GENETICS
... DNA polymerase can add free nucleotides to only the 3 end of the newly-forming strand. This results in elongation of the new strand in a 5'-3' direction. No known DNA polymerase is able to begin a new chain (de novo). DNA polymerase can add a nucleotide onto only a preexisting 3'-OH group, and, ther ...
... DNA polymerase can add free nucleotides to only the 3 end of the newly-forming strand. This results in elongation of the new strand in a 5'-3' direction. No known DNA polymerase is able to begin a new chain (de novo). DNA polymerase can add a nucleotide onto only a preexisting 3'-OH group, and, ther ...
Homework Assignment #1
... 3. (2 pts) RNA polymerase III internal promoters are more than 50 nucleotides downstream of the initiation site. How is RNA polymerase III positioned for correct initiation? Answer: The transcription factor TFIIIC and TFIIIA bind to these internal promoter elements and by themselves do not bind to R ...
... 3. (2 pts) RNA polymerase III internal promoters are more than 50 nucleotides downstream of the initiation site. How is RNA polymerase III positioned for correct initiation? Answer: The transcription factor TFIIIC and TFIIIA bind to these internal promoter elements and by themselves do not bind to R ...
An Overview of Mutation Detection Methods in Genetic Disorders
... Two groups of tests, molecular and cytogenetic, are used in genetic syndromes. In general, single base pair mutations are identified by direct ...
... Two groups of tests, molecular and cytogenetic, are used in genetic syndromes. In general, single base pair mutations are identified by direct ...
HEPATITIS B VIRAL (HBV DNA), QUANTITATIVE
... HBV DNA detected, but below the lower limit of linear range of the assay. These results should be interpreted with caution HBV DNA detected within the linear range of the assay HBV DNA detected above the linear range of the assay ...
... HBV DNA detected, but below the lower limit of linear range of the assay. These results should be interpreted with caution HBV DNA detected within the linear range of the assay HBV DNA detected above the linear range of the assay ...
gene_expression_info
... • Polypeptides are chains of amino acid residues joined by peptide bonds. • Proteins are large polypeptides. • There are 20 different aa and their sequence determines the structure and function of the protein. • The sequence of bases in a DNA molecule determines the sequence of aa. • A gene is a len ...
... • Polypeptides are chains of amino acid residues joined by peptide bonds. • Proteins are large polypeptides. • There are 20 different aa and their sequence determines the structure and function of the protein. • The sequence of bases in a DNA molecule determines the sequence of aa. • A gene is a len ...
Prof. Kamakaka`s Lecture 14 Notes
... –More than half of all coding sequence SNPs result in non-synonymous codon changes. ...
... –More than half of all coding sequence SNPs result in non-synonymous codon changes. ...
DNA as the Genetic Material
... 1. During DNA replication, this is carried out by DNA polymerase, which proofreads each nucleotide against its template as it is added to a strand. When mistake is found, polymerase removes it and resumes synthesis B. Genetic maintenance is also required in DNA 1. Often DNA molecules are subjected t ...
... 1. During DNA replication, this is carried out by DNA polymerase, which proofreads each nucleotide against its template as it is added to a strand. When mistake is found, polymerase removes it and resumes synthesis B. Genetic maintenance is also required in DNA 1. Often DNA molecules are subjected t ...
Nucleic Acids - saddleback.edu
... sugar-phosphate polymers and both have nitrogen-containing bases attached – but there are 3 differences between them. 1) RNA contains a different monosaccharide residue ...
... sugar-phosphate polymers and both have nitrogen-containing bases attached – but there are 3 differences between them. 1) RNA contains a different monosaccharide residue ...
Text S1.
... added to the 3’ end using a Terminal deoxynucleotidyl Transferase. Two PCR reactions were used to amplify the gDNA-Tn junctions and to attach specific sequences required for Illumina sequencing and indexing. The first PCR uses a polyC specific (olj376) and a transposon specific (olj491) primer pair. ...
... added to the 3’ end using a Terminal deoxynucleotidyl Transferase. Two PCR reactions were used to amplify the gDNA-Tn junctions and to attach specific sequences required for Illumina sequencing and indexing. The first PCR uses a polyC specific (olj376) and a transposon specific (olj491) primer pair. ...
HL DNA_Jeopardy 2016
... Draw a line on the graph below to show the relationship between light intensity and the rate of photosynthesis. (2) And identify two things that would be not produced in low light intensity during the Light Dependent reaction that would affect the Calvin Cycle. ...
... Draw a line on the graph below to show the relationship between light intensity and the rate of photosynthesis. (2) And identify two things that would be not produced in low light intensity during the Light Dependent reaction that would affect the Calvin Cycle. ...
Conjugative plasmids are circular pieces of DNA that not only
... Conjugative plasmids are circular pieces of DNA that not only replicate in host bacteria, but also encode machinery to transfer itself into bacteria that do not contain the plasmid. This process is termed conjugation. Nickase is an enzyme that initiates the transfer of the plasmid from “donor” bacte ...
... Conjugative plasmids are circular pieces of DNA that not only replicate in host bacteria, but also encode machinery to transfer itself into bacteria that do not contain the plasmid. This process is termed conjugation. Nickase is an enzyme that initiates the transfer of the plasmid from “donor” bacte ...
SNaPshot® Multiplex System for SNP genotyping
... Capillary electrophoretic analysis of methylation status in CpGrich regions by single-base extension of primers modified with N6-methoxy-2,6-diaminopurine. ...
... Capillary electrophoretic analysis of methylation status in CpGrich regions by single-base extension of primers modified with N6-methoxy-2,6-diaminopurine. ...
Nucleic Acid Deoxyribose Nucleic Acid (DNA)Ribose Nucleic Acid
... form becomes the beta OH that is used to bond to a base unit. • The OH in the second position serves to distinguish between the ribose in RNA and the deoxyribose in DNA. • The OH on the third carbon will bond to the phosphate group of other nucleotides. • The OH group on the fourth carbon is involve ...
... form becomes the beta OH that is used to bond to a base unit. • The OH in the second position serves to distinguish between the ribose in RNA and the deoxyribose in DNA. • The OH on the third carbon will bond to the phosphate group of other nucleotides. • The OH group on the fourth carbon is involve ...
Pathchat no 32 Paternity (rev)
... Markers are named according to their location. If a marker is part of a gene, the gene name is used in the designation. Markers outside gene regions are designated by their chromosomal position, e.g. D5S818 – D is DNA, 5 for chromosome 5, S single copy sequence and the number indicates the order in ...
... Markers are named according to their location. If a marker is part of a gene, the gene name is used in the designation. Markers outside gene regions are designated by their chromosomal position, e.g. D5S818 – D is DNA, 5 for chromosome 5, S single copy sequence and the number indicates the order in ...
File
... Make covalent bonds between nucleotides of the new strands Fast, accurate process Error only one in a billion nucleotides ...
... Make covalent bonds between nucleotides of the new strands Fast, accurate process Error only one in a billion nucleotides ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).