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RNA Interference Case Study - activity
RNA Interference Case Study - activity

... two strands of the siRNA were separated so that one can bind to the mRNA transcript of the Fas gene. This caused the mRNA to be destroyed by enzymes, therefore preventing the gene product from being made. This therapy prevented liver cell death and considerably increased the survival of mice with ...
The Genome of Theobroma Cacao
The Genome of Theobroma Cacao

... Genome sequencing is the process of determining the sequence of consecutive DNA “letters” spanning all of the chromosomes of a cell from start to finish (the four chemical “letters” of DNA are abbreviated A, C, T and G). A computer representation of the genome sequence of cacao appears as 10 long st ...
What is the NUTRIENT needed for growth and repair
What is the NUTRIENT needed for growth and repair

... Exponential increase in the amount of DNA produced in PCR ...
Homology Modeling Zinc Fingers – Introduction zf
Homology Modeling Zinc Fingers – Introduction zf

... model of zinc finger proteins, more specifically zinc finger proteins in the EGR family. The modeled side chains are found to be in a state that is similar to the crystal structure, even in an unbound state, which is particularly important for the key residues involved in DNA recognition. Since the ...
Analysis of Genomes
Analysis of Genomes

... b. by sequence tagged sites (STSs) - unique sites on chromosome, often specific PCR products - if from cDNAs, they’re called expressed sequence tags (ESTs) ...
Across
Across

... 2. the two sides of DNA held together by weak ____ bonds 5. always pairs with cytosine 6. where protein is assembled from the message on the RNA 7. the shape of DNA, double ____ 9. process of copying DNA 13. composed of a sugar, a base, and a phosphate 14. sections of DNA that code for a trait 15. r ...
Study Guide for LS
Study Guide for LS

...  Insertion is when an extra base is added into the sequence.  Deletion is when a base is deleted from the sequence.  Substitution is when one base is substituted for another. Not all mutations are harmful. Some mutations are beneficial, and others have no effect at all. A mutation in DNA could al ...
A Genomic Timeline
A Genomic Timeline

... The National Center for Human Genome Research is created, with the goal of mapping and sequencing all human DNA by 2005. ...
Supplementary Information (doc 63K)
Supplementary Information (doc 63K)

... DNA repair mutants that we report here is reminiscent of that observed in germ cells of telomere replication defective C. elegans mutants(1). One such mutant, trt-1, which has lost functional telomerase reverse transcriptase, shows a decline in transgenerational replicative capacity but not in post- ...
Mitosis Review 2016
Mitosis Review 2016

... 25. What type of cells is produced during mitosis? How does their chromosomes compare to the parent cell? 26. If mitosis did not occur what would be some possible outcomes? ...
Quantification and Sex Determination of Forensic Evidence Materials
Quantification and Sex Determination of Forensic Evidence Materials

... Increase in fluorescence caused by the binding of SYBR® Green to double-stranded DNA is detected during the PCR. The deletion is detected in a dissociation diagram showing the melting temperatures during the PCR cycles. This system has been tested on control samples, mixed samples and casework examp ...
Exon prediction problem using genetic algorithm as an
Exon prediction problem using genetic algorithm as an

... The gene identification problem can be formulated as the deduction of the amino acid sequences encoded in a given DNA genomic sequence [1]. This is an important but difficult problem, especially in eukaryotes, where genes are often split into exons separated by introns. The beginning or end of these ...
Salmonella typhimurium
Salmonella typhimurium

... fibrosis, sickle-cell anaemia; (in bacteria) ability to grow on certain sugars • Many alleles contribute to many traits of an organism such as size, shape, intelligence, behaviour, and risk of getting diseases e.g. (in humans) cancer, heart disease, asthma • Genetic variation is what evolution acts ...
DNA Technology
DNA Technology

... If the cells containing a desired gene translate the gene into protein, then it is possible to identify them by screening for the protein. This if often done by using antibodies that bind to the protein. ...
The Wild World of Biotechnology!! Applications Genetic
The Wild World of Biotechnology!! Applications Genetic

... This is a hit or miss process that requires a great deal of luck to be successful Restriction enzymes generally cut at palindromic DNA sequences ...
Reporting Category 2
Reporting Category 2

... Division of the sex cells (gametes) Makes 4 new haploid cells Haploid vs diploid Crossing over increases genetic diversity so cells are NOT identical ...
Structure of retroviruses
Structure of retroviruses

... 2-The viral envelope formed from the host cell membrane, contains a complex HIV protein that arises through the surface of the virus particle and appears as spiked knobs on electron micrographs of the virus. 3-The virion has a cone-shaped icosahedral core containing the major capsid protein called p ...
Bioinformatics
Bioinformatics

... PROTEIN ...
Biotechnology
Biotechnology

... amino acids ...
variability exercise
variability exercise

... 1. Consider the gene encoding DNA polymerase. All living organisms contain a gene encoding DNA polymerase. ...
Biotechnology
Biotechnology

... two copies of a mutant gene • This gene is called p53. The mutated version is linked to colon cancer. • How do you think we will go about this? – RFLPs and gel electrophoresis ...
Transcription and Translation Exercise
Transcription and Translation Exercise

... 5. If a protein has 150 amino acids, how many DNA nucleotides would make up the coding region of the gene? ...
deoxyribonucleic acid contained in the chromosomes humans have
deoxyribonucleic acid contained in the chromosomes humans have

... ­humans have 46, dogs­78, mice­40, some  bacteria­only one ...
How does it all work
How does it all work

... Ex. A, T, G, C • Each group of three bases codes for one amino acid See page 128 in your book! ...
DNA vs RNA
DNA vs RNA

... ADENINE (A) = THYMINE (T) GUANINE (G) = CYTOSINE (C) B Rosalind Franklin (1952) Took an X-ray of the DNA structure so the patterns could be seen. THE X-RAYS SHOW THAT DNA IS TWISTED AROUND EACH OTHER LIKE A HELIX AND HAS 2 STRANDS. X-ray of DNA ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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