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Genetic Engineering
Genetic Engineering

... a.) DNA extraction – simple chemical process to get DNA out of cell; cells are opened & DNA is separated from other cell parts b.) cutting DNA – restriction enzymes are used to cut DNA at specific sequences of nucleotides ...
Document
Document

... Hierarchical shotgun method Assemble contigs from various chromosomes, then sequence and assemble them. A contig is a set of overlapping clones or sequences from which a sequence can be obtained. The sequence may be draft or finished. A contig is thus a chromosome map showing the locations of those ...
Lecture 1 - Graham Ellis
Lecture 1 - Graham Ellis

... Why is DNA important? 1. DNA contains the instructions needed to construct other components of cells such as protein and RNA. 2. There are 20 different kinds of amino acid that combine to make proteins. There are many possible combinations, resulting in many different types of protein. 3. The cell ...
PowerPoint® slides
PowerPoint® slides

... LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. University will not be liable for any costs, damages, fees or other liability, nor for any direct, indirect, special, incidental or consequential damages (including lost profits) with respect to any claims by ...
Chapter 13 Selective breeding is a technique of choosing specific
Chapter 13 Selective breeding is a technique of choosing specific

... Sometimes it can be harmful if two genetically similar organisms have recessive genes for a genetic defect. For example, dalmations are known for hearing problems that have resulted from inbreeding for generations. Genetic engineering is the intentional changing of an organism's DNA to increase vari ...
Go to - Net Start Class
Go to - Net Start Class

... The various controls when clicked highlight parts of the DNA molecule or move it into different positions. The students can also use the mouse to grab the DNA to move it to see its structure. The color legend is given when you “click for explanation” under C H O N P. For example, clicking “Backbone ...
DNA Notesheet
DNA Notesheet

... 19. In what two ways is mRNA different from the DNA it is copying? 1_ 2_ 20. What is polymerase? 21. If the DNA strand being copied is T-G-C-A-G-T, what would be the mRNA strand? 22. What is transcription? _ 23. After detaching from DNA, mRNA leaves the ...
Microbial Genetics and Taxonomy
Microbial Genetics and Taxonomy

... Genetic screening - for inherited forms of breast CA DNA fingerprinting – use gel electrophoresis and southern blotting to identify unique DNA sequences of individuals or organisms Gene therapy – missing or defective genes are replaced with normal genes (in SCID- Severe Combined Immunodeficiency) Me ...
DNA_NOTES
DNA_NOTES

... • The ribosome looks for the "start" _________ - AUG, this is where the chain begins • ____________________ has an anticodon at one end and an amino acid at the other, it binds to a complementary codon. Draw a tRNA molecule to the right. • Translate the following mRNA to tRNA: ...
PowerPoint Slides
PowerPoint Slides

... The Helios Gene Gun is a new way for in vivo transformation of cells or organisms (i.e. gene therapy and genetic immunization (DNA vaccination)). This gun uses Biolistic ® particle bombardment where DNA- or RNA-coated gold particles are loaded into the gun and you pull the trigger. A low pressure he ...
DNA Similarities
DNA Similarities

... pairs). How much similarity would you expect between a whale and a fish? A whale and a dog? A dog and a shrimp? A shrimp and a bacterium? As always, there are two types of similarity to be considered: analogy and homology. If the sequences show similarity because they code for proteins with similar ...
Review article Zinc finger protein (ZFP) in plants
Review article Zinc finger protein (ZFP) in plants

... How do the ZFP transcription factors work? Regulation of gene expression is controlled by the sequence-specific binding of transcription factors to their DNA recognition sites, which is a key step in the regulation of networks. For instance, CCCH-type zinc finger is capable of binding to the 5’-UAUU ...
Nucleotides and DNA Structure
Nucleotides and DNA Structure

... A) hydrogen bonding between purines. B) the phosphodiester backbone. C) Ionic nucleobase attraction. D) the stacking interactions between base pairs. ...
Molecules of Life
Molecules of Life

... 2. Suggest why ribosomes can’t be seen using a light microscope. 3. Why was Rosalind Franklin’s work so important in the discovery of DNA? 4. Why is it important that scientists repeat the work of other scientists. 5. As DNA is made of two strands, can you think of a possible copying mechanism it ma ...
A Bacterial Plasmid: What can you tell me about the plamid?
A Bacterial Plasmid: What can you tell me about the plamid?

... organism’s DNA. Create sticky ends that are complementary to the plasmid’s sticky ends. • Insert the gene using ligase. How does one determine which RE’s to use? ...
Ch 020 DNA Technology II
Ch 020 DNA Technology II

... Restriction enzymes (endonucleases): in nature, these enzymes protect bacteria from intruding DNA; they cut up the DNA (restriction); very specific Restriction site: recognition sequence for a particular restriction enzyme Restriction fragments: segments of DNA cut by restriction enzymes in a reprod ...
Exam Review 2B -- Rodermel
Exam Review 2B -- Rodermel

... Attach to single-stranded DNA and prevent secondary structures from forming Unwinds DNA at replication fork ...
DNA Structure
DNA Structure

... • Central Dogma of Molecular Genetics: • DNA makes RNA which makes protein • To be a genetic molecule, DNA must: replicate, store info, express info, vary by mutation ...
Leukaemia Section t(11;20)(q23;q11) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(11;20)(q23;q11) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... Schematic representation of MLL-EB1 and EB1-MLL fusion proteins. AT-H, AT hooks; MT, DNA methyltansferase motif; Zn-F, zinc finger domain; SET, [Su(var]3-9, enhancer of zeste, and trithorax] domain; En, microtubule-binding domain; ABD, APC-binding domain; numbers indicate amino acids of each protein ...
Chapter 15 – Recombinant DNA and Genetic Engineering
Chapter 15 – Recombinant DNA and Genetic Engineering

... • Gene Therapy: transfer of one or more modified genes into an individual’s cells – Correct genetic defect – Boost immune system • Recombinant DNA Technology: science of cutting and recombining DNA from different species – Genes are then placed into bacterial, yeast or mammalian cells and replicated ...
Cloning the Progesterone 5 beta- reductase gene
Cloning the Progesterone 5 beta- reductase gene

... We soon found that the Butterfly we chose (the Monarch) does not actually produce the toxin itself but rather sequesters it from its food source, the Milkweed, as a larvae and pupa. The milkweed itself did not have a gene on file that we could isolate so we did some research on what exactly this tox ...
EOC Review Chapters6
EOC Review Chapters6

... used in the future so the DNA strand will shorten. C. The DNA will be exactly like the original since only the original strand of DNA is used as a template. D. When the new strand is used as a template the strand built from it will differ from the original DNA. ...
Impacts of DNA-based technologies and PCR basics
Impacts of DNA-based technologies and PCR basics

... Proteins (and some RNAs) compose cells Cells compose organisms ...
Document
Document

... Likewise, in an effort to confirm that gene editing was at least possible, cells from rice plants were transformed with vectors carrying CRISPR gateway vector targeting CHLOROPHYLL A ...
DNA Technology
DNA Technology

... ABC’s “Family Matters” • Steve Urkel (nerd) altered his DNA to become Stefan Urquelle (cool guy) to win the affections of Laura Winslow (this upset his present girlfriend Myra Munkhouse). This would be an example of genetic ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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