No Slide Title
... • DNA is fractionated, 5’ biotin labeled and attached to streptavidin coated beads. The non-labeled strand is released. • The DNA on each bead is amplified onto the bead • One bead is deposited in each well • Pyrosequencing takes place in the ...
... • DNA is fractionated, 5’ biotin labeled and attached to streptavidin coated beads. The non-labeled strand is released. • The DNA on each bead is amplified onto the bead • One bead is deposited in each well • Pyrosequencing takes place in the ...
Gene Tagging with Transposons
... back to wild type (ie, colonies kept turning blue) • lac- mutants were due to transposons which then moved back out of the gene Agar w/X-Gal ...
... back to wild type (ie, colonies kept turning blue) • lac- mutants were due to transposons which then moved back out of the gene Agar w/X-Gal ...
Evolution of prokaryotic genomes
... (d) Site-specific recombination can generate genetic variation upon use of secondary crossing-over sites Site-specific DNA inversion systems have been thoroughly studied both in vivo and in vitro with bacteria, bacteriophages and plasmids (Glasgow et al., 1989). An example is the periodic inversion ...
... (d) Site-specific recombination can generate genetic variation upon use of secondary crossing-over sites Site-specific DNA inversion systems have been thoroughly studied both in vivo and in vitro with bacteria, bacteriophages and plasmids (Glasgow et al., 1989). An example is the periodic inversion ...
document
... Every enzyme has a set of physical conditions (temperature, pH, etc.) at which it performs best. 37C is human body temperature. These bacteria live in the human body. The enzymes have evolved to function best at this temperature. 7) What is a restriction digest and what does it have to do with gel ...
... Every enzyme has a set of physical conditions (temperature, pH, etc.) at which it performs best. 37C is human body temperature. These bacteria live in the human body. The enzymes have evolved to function best at this temperature. 7) What is a restriction digest and what does it have to do with gel ...
Mutation PPT
... base pairs in a particular sequence. • An allele is one variant of that instruction. ...
... base pairs in a particular sequence. • An allele is one variant of that instruction. ...
HMG B domain
... and HMGA1b (HMGY). These two are identical in sequence except for a deletion of 11 residues between the the first and second AT hook in the latter. Alternative splicing also produces HMGA1c. The related HMGA2 (HMGI-C) protein is coded for by a separate gene. ...
... and HMGA1b (HMGY). These two are identical in sequence except for a deletion of 11 residues between the the first and second AT hook in the latter. Alternative splicing also produces HMGA1c. The related HMGA2 (HMGI-C) protein is coded for by a separate gene. ...
the DNA Binding Lab Lesson Plan Powerpoint
... Why study how molecules interact with DNA? 1. All genetic information is stored in DNA A. Proteins bind to DNA to use information B. Proteins bind DNA to copy it C. Proteins bind DNA to repair it ...
... Why study how molecules interact with DNA? 1. All genetic information is stored in DNA A. Proteins bind to DNA to use information B. Proteins bind DNA to copy it C. Proteins bind DNA to repair it ...
Communication
... Describe how sections of DNA containing a desired gene can be extracted from a donor organism using restriction enzymes. ...
... Describe how sections of DNA containing a desired gene can be extracted from a donor organism using restriction enzymes. ...
Chapter 12
... – One source contains the gene that will be cloned – Another source is a gene carrier, called a vector – Plasmids (small, circular DNA molecules independent of the bacterial chromosome) are often used as vectors ...
... – One source contains the gene that will be cloned – Another source is a gene carrier, called a vector – Plasmids (small, circular DNA molecules independent of the bacterial chromosome) are often used as vectors ...
FOSL - Biotechnology Unit Date During class we will… Outside of
... HS- LS1-6: Construct and revise an explanation based on evidence for how carbon, hydrogen, and oxygen from sugar molecules may combine with other elements to form amino acids and/or other large carbon-based molecules. HS-LS3-1: Ask questions to clarify relationships about the role of DNA and chromos ...
... HS- LS1-6: Construct and revise an explanation based on evidence for how carbon, hydrogen, and oxygen from sugar molecules may combine with other elements to form amino acids and/or other large carbon-based molecules. HS-LS3-1: Ask questions to clarify relationships about the role of DNA and chromos ...
Answers to Conceptual Questions C1. Answer: First
... primer would be complementary to the 5′ end of the mRNA and would be unique to the βglobin sequence. The other primer would be complementary to the 3′ end. This second primer could be a poly-dT primer or it could be a unique primer that would bind slightly upstream from the polyA-tail region. E13. A ...
... primer would be complementary to the 5′ end of the mRNA and would be unique to the βglobin sequence. The other primer would be complementary to the 3′ end. This second primer could be a poly-dT primer or it could be a unique primer that would bind slightly upstream from the polyA-tail region. E13. A ...
Gene
... genes have taken over important human functions, such as regulating responses to stress. "This is kind of a shocker and will no doubt inspire some further study," Collins says. Indeed, scientists previously thought that this kind of horizontal gene transfer was not possible in vertebrates. Another c ...
... genes have taken over important human functions, such as regulating responses to stress. "This is kind of a shocker and will no doubt inspire some further study," Collins says. Indeed, scientists previously thought that this kind of horizontal gene transfer was not possible in vertebrates. Another c ...
Slide 1
... 12.11 The analysis of genetic markers can produce a DNA profile DNA profiling is the analysis of DNA fragments to determine whether they come from the same individual. DNA profiling – compares genetic markers from noncoding regions that show variation between individuals and ...
... 12.11 The analysis of genetic markers can produce a DNA profile DNA profiling is the analysis of DNA fragments to determine whether they come from the same individual. DNA profiling – compares genetic markers from noncoding regions that show variation between individuals and ...
Selective propagation of the clones
... Lamda DNA transfection efficiency low; when packed more efficient. ...
... Lamda DNA transfection efficiency low; when packed more efficient. ...
Activity--Extracting DNA - e
... In the Space Station Alpha mission preparation, your students learned that the solar storm represents a great danger to the astronauts if they are not shielded from the damaging radiation of the sun. The solar rays can penetrate the layers of the space station and damage the astronauts’ DNA, the gen ...
... In the Space Station Alpha mission preparation, your students learned that the solar storm represents a great danger to the astronauts if they are not shielded from the damaging radiation of the sun. The solar rays can penetrate the layers of the space station and damage the astronauts’ DNA, the gen ...
Promoters - Pennsylvania State University
... The radioactively-labeled DNA probe binds two proteins. Each is sequence specific. ...
... The radioactively-labeled DNA probe binds two proteins. Each is sequence specific. ...
Reproductive Technology
... – Known genes are labeled on Human Genome Browser (www.genome.ucsc.edu) – Gene-like sequences are searched for to try to identify position of unknown genes ...
... – Known genes are labeled on Human Genome Browser (www.genome.ucsc.edu) – Gene-like sequences are searched for to try to identify position of unknown genes ...
... Due October 14, 2015 For example one chromosome could look like this, with three tandem repeat (see above), while a chromosome might have four, giving a larger PCR product. Note that since we have two copies of each chromosome there are two possible PCR products, one from each chromosome. It is poss ...
Lecture#23 - Cloning genes by complementation
... easily, typically on a Petri dish. relies on the cloned gene providing a functional gene product that is absent/nonfunctional in the mutant host -> complementation. Example: Cloning a gene for an E. coli auxotrophic mutant - mutant in some gene called "A". ...
... easily, typically on a Petri dish. relies on the cloned gene providing a functional gene product that is absent/nonfunctional in the mutant host -> complementation. Example: Cloning a gene for an E. coli auxotrophic mutant - mutant in some gene called "A". ...
Types of Restriction Endonucleases | NEB
... Type II enzymes cut DNA at defined positions close to or within their recognition sequences. They produce discrete restriction fragments and distinct gel banding patterns, and they are the only class used in the laboratory for routine DNA analysis and gene cloning. Rather than forming a single famil ...
... Type II enzymes cut DNA at defined positions close to or within their recognition sequences. They produce discrete restriction fragments and distinct gel banding patterns, and they are the only class used in the laboratory for routine DNA analysis and gene cloning. Rather than forming a single famil ...
DNA sequencing
... Genome variations include mutations and polymorphisms. Technically, a polymorphism (a term that comes from the Greek words "poly," or "many," and "morphe," or "form") is a DNA variation in which each possible sequence is present in at least 1% of people. For example, a place in the genome where 93 p ...
... Genome variations include mutations and polymorphisms. Technically, a polymorphism (a term that comes from the Greek words "poly," or "many," and "morphe," or "form") is a DNA variation in which each possible sequence is present in at least 1% of people. For example, a place in the genome where 93 p ...
BIOTECHNOLOGY
... cut DNA in a predictable and precise manner, at a specific nucleotide sequence called a recognition site . Hamilton Smith, John Hopkins University, won the Nobel Prize in 1978 for discovering restriction enzymes in bacteria (Hind III). He found their main purpose was to cut foreign DNA that tried to ...
... cut DNA in a predictable and precise manner, at a specific nucleotide sequence called a recognition site . Hamilton Smith, John Hopkins University, won the Nobel Prize in 1978 for discovering restriction enzymes in bacteria (Hind III). He found their main purpose was to cut foreign DNA that tried to ...
DNA Notes.pps
... 5. What are the main differences between DNA and RNA. DNA has deoxyribose, RNA has ribose; DNA has 2 strands, RNA has one strand; DNA has thymine, RNA has uracil. 6. Using the chart on page 303, identify the amino acids coded for by these codons: ...
... 5. What are the main differences between DNA and RNA. DNA has deoxyribose, RNA has ribose; DNA has 2 strands, RNA has one strand; DNA has thymine, RNA has uracil. 6. Using the chart on page 303, identify the amino acids coded for by these codons: ...
Document
... E2. The plasmid with the wrong orientation would not work because the coding sequence would be in the wrong direction relative to the promoter sequence. Therefore, the region containing the somatostatin sequence would not be transcribed into RNA. E3. One possibility is to clone the toxin-producing g ...
... E2. The plasmid with the wrong orientation would not work because the coding sequence would be in the wrong direction relative to the promoter sequence. Therefore, the region containing the somatostatin sequence would not be transcribed into RNA. E3. One possibility is to clone the toxin-producing g ...