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DNA Structure and Replication Notes
DNA Structure and Replication Notes

... • On the ends of the chromosomes in eukaryotes are structures called telomeres that are repeating bases that protect the DNA ...
Genetic Engineering
Genetic Engineering

... 4. Combine the cut pieces of DNA together and ...
Genetic Engineering
Genetic Engineering

... 4. Combine the cut pieces of DNA together and ...
251-06 Exam1 2-15
251-06 Exam1 2-15

... The first base of the sequence is the +1 startsite. (1) Does the sequence shown above contain a promoter? Why or why not? This sequence cannot contain a promoter, since it begins at the +1 startsite of transcription. The promoter lies upstream (to the left of) the startsite. The promoter DNA sequenc ...
Human Genome Project
Human Genome Project

... How the Human Compares with Other Organisms •Unlike the human's seemingly random distribution of gene-rich areas, many other organisms' genomes are more uniform, with genes evenly spaced throughout. •Humans have on average three times as many kinds of proteins as the fly or worm because of mRNA tra ...
Harlem DNA Lab brochure
Harlem DNA Lab brochure

... The DNA Learning Center (DNALC) is the world’s first science center devoted entirely to genetics education. The DNALC is an operating unit of Cold Spring Harbor Laboratory (CSHL), a world-renowned research institution that has been home to eight Nobel Prize winners. CSHL is ranked number one in the ...
aneuploidy
aneuploidy

...  Sometimes this can cause no change. Sometimes it can produce a new A.A.  It may or may not interfere with protein synthesis. ...
File - Groby Bio Page
File - Groby Bio Page

... colony, which has lost resistance to tetracycline. This must be a colony of cells which have taken up the recombinant plasmid! ...
Better Crush and Soak, than Crash and Burn!
Better Crush and Soak, than Crash and Burn!

... gels. Perhaps, the most common non-commercial method is the isolation of DNA by the crush and soak method. Although commercially available kits are very convenient and affordable, there are conditions where I personally avoid using these kits. During the gel extraction of small DNA fragments (~200 b ...
What is gene cloning?
What is gene cloning?

... - Independent existence in the bacterial cells - Carry antibiotics resistance genes : ex) ampicillin resistant gene (AmpR) ...
Biology 207 Workshop 9
Biology 207 Workshop 9

... were crossed with albinos of genotype ccbb, three phenotypes were produced: black 102; brown 198; albino 300 a. Explain why one can conclude that the two genes are linked. b. Calculate the percentage recombination between the two genes. c. If each of the 102 black offspring is used as a parent in a ...
DNA Technology
DNA Technology

... The chemical structure of everyone's DNA is the same. The only difference between people (or any animal) is the order of the base pairs. Using these sequences, every person could be identified solely by the sequence of their base pairs. However, because there are so many millions of base pairs, the ...
Antiviral agents
Antiviral agents

...  Extensive studies carried out on the life cycle of HIV ...
Classification of DNA sequences using Bloom Filters
Classification of DNA sequences using Bloom Filters

... Classification of DNA sequences using Bloom Filters • New generation sequencing technologies – Complex datasets – New efficient, specialized sequence analysis algorithms ...
2014
2014

... DNA duplex by the polymerase then forms the ___________________ complex. After elongation, termination of transcription can occur with a ρ-independent mechanism with the formation of both a ______________ and __________________. Alternatively in the ρ-dependent mechanism, a rut site is recognized by ...
Clone
Clone

... modified to carry new genes • Plasmids useful as cloning vectors must have • a replicator (origin of replication) • a selectable marker (antibiotic resistance gene) • a cloning site (site where insertion of foreign DNA will not disrupt replication or inactivate ...
DISCOVERY OF DNA
DISCOVERY OF DNA

... 5. The S strain and the R strain of S. pneumoniae are different in that a. the R strain produces a capsule but the S strain does not. b. the S strain produces a capsule but the R strain does not. c. the R strain is virulent but the S strain is not. d. the R strain contains protein but the S strain d ...
Evolution of DNA by celluLar automata HC Lee Department of
Evolution of DNA by celluLar automata HC Lee Department of

... • Wolfram: simple rules can lead to complex systems ...
Mutation Notes:
Mutation Notes:

... • Which type of mutation is more serious? – Frameshift mutation affects every amino acid after the mutation – Point mutation affects only the amino acid at the mutation ...
DNA, restriction enzymes
DNA, restriction enzymes

... Polypeptide amide bonds, like ester bonds, are labile to either acid- or base-catalyzed hydrolysis. Ester bonds in lipids are hydrolyzed, and so fats and phospholipids are saponified. RNA is hydrolysed by base, because deprotonation of the ribose 2'-OH group allows the 2' O atom to attack the P of t ...
DNA Structure
DNA Structure

... AT rich fragment less stable 2 H-bonds/bp versus 3 H-bonds/bp ...
pGLO2011 Wilkes
pGLO2011 Wilkes

... transforming principle from pneumococcus. Biochemical tests revealed it to be deoxyribonucleic acid (DNA). Taken together, all this evidence pointed to DNA as the components of genes. ...
chapter 8 and 9
chapter 8 and 9

... Example: nitrous acid strips the amino group from nucleotides Base analogs Resemble nucleotide bases; erroneously incorporated into DNA Analog base-pairs with a different nucleotide Intercalating agents Insert between base-pairs, pushing nucleotides apart; extra nucleotide may then be erroneously ad ...
Study Questions 2
Study Questions 2

... DNA in cells deviates from the ideal B form by having increased overall pitch, with an average of approximately 10.5 base pairs per turn instead of 10 in the ideal B form. In addition, DNA in solution is irregular, including deviations at the level of the co-planarity of the base pairs (propeller tw ...
DNA sequencing - University of Louisville Bioinformatics
DNA sequencing - University of Louisville Bioinformatics

... could be interpreted in terms of genetic code. • Analysis of mutations in genes identified by traditional phage genetics combined with amino acids allowed phage genes to be located on the DNA sequence. • For the first time, DNA sequence identified long open reading frames that could be assigned to g ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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