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CH 16 Vocab
CH 16 Vocab

... A short stretch of RNA with a free 3' end, bound by complementary base pairing to the template strand, that is elongated with DNA nucleotides during DNA replication. Pyrimidine One of two types of nitrogenous bases found in nucleotides, characterized by a six-membered ring. Cytosine (C), thymine (T) ...
Something`s Fishy
Something`s Fishy

... You have learned that DNA is a linear sequence of nucleotides made up of adenine, thymine, guanine, and cytosine. This sequence of A, T, G, and C is unique to each individual. Restriction enzymes cut DNA. Each restriction enzyme recognizes a specific group of “target” base pairs and makes a cut with ...
Nucleic Acid Biotechnology Techniques
Nucleic Acid Biotechnology Techniques

... • Plasmid vectors pBR322 and pUC are cloning ...
computational biology
computational biology

... Since protein-coding DNA is divided into codons three bases long, insertions and deletions can alter a gene so that its message is no longer correctly parsed. These changes are called frameshifts. There are other types of mutations as well, but this short list should give you an idea of the ...
mb_ch13
mb_ch13

... The Future of Genomics, continued • Microarrays – DNA microarrays, two-dimensional arrangements of cloned genes, allow researchers to compare specific genes such as those that cause cancer. ...
Reviews - Mi Portal
Reviews - Mi Portal

... even if a cell with an unrepaired DSB continues to divide, the broken chromosome fragments will mis-segregate and be degraded, producing aneuploidy. In response to this threat, cells have elaborated an impressive arsenal of DNA-repair pathways. There are two general types of repair: homologous recom ...
Mechanism of Surface Stress due to DNA strands on Gold
Mechanism of Surface Stress due to DNA strands on Gold

... • At the end of each cycle, the amount of DNA has doubled • By the end of 30 cycles, you will have about 1 billion molecules from the original one you started with!! ...
CH 16 and 17 PowerPoint
CH 16 and 17 PowerPoint

... • characterize the gene regulatory networks that control these machines • characterize the functional repertoire of complex microbial communities in their ...
Figure S5.
Figure S5.

... ...
dna_2
dna_2

... • Finally, they destroyed the DNA and injected the extract into the mice. The mice survived. • DNA was the transforming factor. • Avery and the other scientists discovered that DNA stores and transmits the genetic information from one generation of an organism to the next. ...
View ePoster - 2015 AGU Fall Meeting
View ePoster - 2015 AGU Fall Meeting

... methyl-coenzyme M reductase alpha subunit (mcrA). PmoA genes of Type II methanotrophs were found three times more than Type I methanotrophs. A pmoA gene sequence represents 42% of the library matches only and is identical to a putative protein sequence annotated on Ca. D. audaxviator genome, but fur ...
What`s the Point - Biology Junction
What`s the Point - Biology Junction

... 5. Insert and extra thymine base (T) in the 7th position (after the sixth T and the next letter C). Write the new base sequence (16 letters total). New DNA base sequence: _____________________________________ 6. Determine the mRNA and amino acid sequence made from this changed DNA Gene #5. REMEMBER, ...
PP4 (Ch.12-25)DNA
PP4 (Ch.12-25)DNA

... •Anti-parallel to each other •Always builds 5’ to 3’ •DNA Polymerase can only add to the 3’ end If DNA were synthesized in the 3' to 5' direction, the energy for the process would come from the 5' end of the growing strand rather than from free nucleotides. If the 5' nucleotide needed to be removed ...
How is genome sequencing done
How is genome sequencing done

... Millions of DNA Sequences – Long Reads with 454 Sequencing™ Reference DNA Sequence (genome or chromosome) ...
replicate, transcribe, translate
replicate, transcribe, translate

... DNA replication is the process cells use to make new DNA, and is semi-conservative in that each new DNA double-helix formed contains half of the DNA strand replicated. Replication as it occurs within cells requires a DNA template, energy provided by nucleoside triphosphate molecules (dNTPs and rNTPs ...
DNA/RNA/Protein Synthesis Pre-Test
DNA/RNA/Protein Synthesis Pre-Test

... A. Found that A=T and C=G B. Found that traits of bacteria were passed from parents to offspring C. Used X-Ray to reveal structure of crystals D. Found that DNA was made of more than 1 nucleotide; DNA had a backbone made of phosphate and sugar E. Determined that actual structure of DNA; 1 strand is ...
Presentación de PowerPoint - International Potato Center
Presentación de PowerPoint - International Potato Center

... supported phylogenetic groups. Group 4 is new as compared to Arabidopsis and is clearly distinguished by a a different type of zinc finger motif with the C-X4C motif typical to Group 2 but with a H-X-C motif typical for group 3. Groups 2d and 2e each form well supported phylogenetic groups. Group 4 ...
DNA conformation and restriction enzyme activity
DNA conformation and restriction enzyme activity

... from HeLa cells on DEAE-Bio-Gel. These enzymes show similar preferences for activated and denatured D N A to those of our a,E/a,T, a,E and a,T respectively. Those authors showed that polymerase a2contains two accessory subunits, C 1 and C2, which confer on it the ability to utilize single-stranded D ...
Chapter 18 Gene Expression and Protein Synthesis
Chapter 18 Gene Expression and Protein Synthesis

... mutagens do not become mutations because cells have repair mechanisms called nucleotide excision repair (NER). • NER can prevent mutations by cutting out damaged areas and resynthesizing the proper sequence. ...
Chromothripsis: how does such a catastrophic event impact human
Chromothripsis: how does such a catastrophic event impact human

... de novo chromosomal structural aberrations found at term (Pellestor et al., 2011a). This confirms the great vulnerability of spermatogenesis to DNA damage and its limited or less efficient DNA repair capacity when compared with somatic tissue cells. In the course of spermatogenesis and according to th ...
Chromosomal Mutations
Chromosomal Mutations

... Effects of Mutations • Any new trait in a population, good or bad, is a result of a mutation! • Neutral: no effect on protein function • Harmful: cause genetic diseases • Beneficial: gives the organism a better chance of ...
Artemis as genome viewing and annotation tool
Artemis as genome viewing and annotation tool

... Overview of the genome sequencing and sequence analysis. Demonstration of Artemis. Hands on guided exercise in Artemis. Demonstration of ACT . Hands on guided exercise in ACT Generating ACT comparison files ...
13.2 – Manipulating DNA
13.2 – Manipulating DNA

...  Sometimes called "molecular photocopying," the polymerase chain reaction (PCR) is a fast and inexpensive technique used to "amplify" - copy - small segments of DNA.  Because significant amounts of a sample of DNA are necessary for molecular and genetic analyses, studies of isolated pieces of DNA ...
LECT14 DNA
LECT14 DNA

... Is base-pairing the same? Yes. But the bases join around the axis and not through the axis and are tilted 20 degrees. Why is A DNA important to know? A DNA is seen in single-stranded RNA molecules that fold back on themselves. A DNA is also seen in DNA-RNA hybrids. Low humidity causes it to form fro ...
Human Genetics Traits lab
Human Genetics Traits lab

... that the placing the left over the right is due to a dominant gene (F) while placing the right on top occurs only in the recessive (ff) genotype. Long Palmar Muscle When a person is homozygous for a recessive gene (l) they have a long palmar muscle. This muscle can be detected by examination of the ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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