Section 7.1 DNA Cloning with Plasmid Vectors
... The essence of cell chemistry is to isolate a particular cellular component and then analyze its chemical structure and activity. In the case of DNA, this is feasible for relatively short molecules such as the genomes of small viruses. But genomes of even the simplest cells are much too large to dir ...
... The essence of cell chemistry is to isolate a particular cellular component and then analyze its chemical structure and activity. In the case of DNA, this is feasible for relatively short molecules such as the genomes of small viruses. But genomes of even the simplest cells are much too large to dir ...
Communication: Formation of Knots in Partially Replicated DNA
... DiNardo et al., 1984; Rybenkov et al., 1997; Wang, 1991). In a crowded cellular environment or in in vitro reactions topoisomerases can lead to inadvertent formation of DNA knots (Dean et al., 1985; Shishido et al., 1987). Under in vivo conditions, DNA knots are usually rare and short-lived as furth ...
... DiNardo et al., 1984; Rybenkov et al., 1997; Wang, 1991). In a crowded cellular environment or in in vitro reactions topoisomerases can lead to inadvertent formation of DNA knots (Dean et al., 1985; Shishido et al., 1987). Under in vivo conditions, DNA knots are usually rare and short-lived as furth ...
Multifractal analysis of DNA sequences using a novel chaos
... other dimension-like and entropy-like measures, inspired by the information-theoretical measures that are widely used in linguistics [31,32], the analysis of local singularities does not focus on a single exponent, but considers all the scaling behaviors within the sequence. Moreover, some of these ...
... other dimension-like and entropy-like measures, inspired by the information-theoretical measures that are widely used in linguistics [31,32], the analysis of local singularities does not focus on a single exponent, but considers all the scaling behaviors within the sequence. Moreover, some of these ...
IV. Enzymology of DNA Replication
... located near the top of the tube after ultracentrifugation b) DNA from bacteria grown for only one generation on heavy nitrogen showed only one band that was located near the middle of the tube after ultracentrifugation c) DNA from bacteria grown for two generations on heavy nitrogen showed two band ...
... located near the top of the tube after ultracentrifugation b) DNA from bacteria grown for only one generation on heavy nitrogen showed only one band that was located near the middle of the tube after ultracentrifugation c) DNA from bacteria grown for two generations on heavy nitrogen showed two band ...
Paper
... Joseph K. Pickrell,1* Yoav Gilad,1 Jonathan K. Pritchard1,2 Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechani ...
... Joseph K. Pickrell,1* Yoav Gilad,1 Jonathan K. Pritchard1,2 Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechani ...
Reaction of Systemic Lupus Erythematosus Antinative DNA
... hold, N. J.). Pancreatic DNAse andl mon-onucleotides were mA/gel for 4.5 h; this amperage avoided heating and denafrom Sigma Chemical Co. (St. Louis, NM.), and SI nuclease turation of the DNA fragments. Horizontal sections 4-mm thick of Aspergilltus orylzae, from Miles Laboratories Inc., Ames were c ...
... hold, N. J.). Pancreatic DNAse andl mon-onucleotides were mA/gel for 4.5 h; this amperage avoided heating and denafrom Sigma Chemical Co. (St. Louis, NM.), and SI nuclease turation of the DNA fragments. Horizontal sections 4-mm thick of Aspergilltus orylzae, from Miles Laboratories Inc., Ames were c ...
Characterization of two rice DNA methyltransferases
... colony-lifts and hybridization to radiolabeled probes using standard procedures. The following probes were used to obtain the various subclones: R8 probe for the H1 fragment, ES probe for the S1 fragment, SN probe for the H2 fragment, PS probe for the S2 fragment and BH probe for the S3 fragment (se ...
... colony-lifts and hybridization to radiolabeled probes using standard procedures. The following probes were used to obtain the various subclones: R8 probe for the H1 fragment, ES probe for the S1 fragment, SN probe for the H2 fragment, PS probe for the S2 fragment and BH probe for the S3 fragment (se ...
transformation
... a culture medium to produce many copies of the bacterium. The gene is activated and the bacterium begins to produce the protein that the gene codes for. One real-life application of this is the production of human insulin by bacteria. In this activity, students will model how DNA transformation work ...
... a culture medium to produce many copies of the bacterium. The gene is activated and the bacterium begins to produce the protein that the gene codes for. One real-life application of this is the production of human insulin by bacteria. In this activity, students will model how DNA transformation work ...
Large Scale SNP Scanning on Human Chromosome Y and DNA
... include their multiple loci and incomplete penetration. To pinpoint these loci in terms of particular regions of the chromosomes, association studies, which compare allele frequency between affected individuals (probands) and controls, must be performed across the entire human genome. With approxima ...
... include their multiple loci and incomplete penetration. To pinpoint these loci in terms of particular regions of the chromosomes, association studies, which compare allele frequency between affected individuals (probands) and controls, must be performed across the entire human genome. With approxima ...
Nucleic Acids | Principles of Biology from Nature Education
... Structure of Nucleic Acids The human body contains an amazing variety of different types of cells that originate from just one fertilized egg. But how does that single cell manage to grow and differentiate into the diverse cells that make up a human being? All the information needed for this amazing ...
... Structure of Nucleic Acids The human body contains an amazing variety of different types of cells that originate from just one fertilized egg. But how does that single cell manage to grow and differentiate into the diverse cells that make up a human being? All the information needed for this amazing ...
DNA - Miss Schwippert
... molecule is composed of one “old” strand and one “new” strand (Here a “strand” refers to one chain of nucleotides) ...
... molecule is composed of one “old” strand and one “new” strand (Here a “strand” refers to one chain of nucleotides) ...
Amplification and partial sequencing of Ixodes Scapularis Shaker
... passing pathogens to humans that cause lyme disease, rocky mountain spotted fever and tularemia (9). The research project undertaken provides an easy and efficient means to begin the tick DNA sequencing on a small scale, which can be done in almost any molecular biology laboratory. The sequence homo ...
... passing pathogens to humans that cause lyme disease, rocky mountain spotted fever and tularemia (9). The research project undertaken provides an easy and efficient means to begin the tick DNA sequencing on a small scale, which can be done in almost any molecular biology laboratory. The sequence homo ...
DNA Sequencing
... Only a cell that took up a plasmid, which has the ampR gene, will reproduce and form a colony. Colonies with nonrecombinant plasmids will be blue, because they can hydrolyze X-gal. Colonies with recombinant plasmids, in which lacZ is disrupted, will be white, because they cannot hydrolyze X-gal. By ...
... Only a cell that took up a plasmid, which has the ampR gene, will reproduce and form a colony. Colonies with nonrecombinant plasmids will be blue, because they can hydrolyze X-gal. Colonies with recombinant plasmids, in which lacZ is disrupted, will be white, because they cannot hydrolyze X-gal. By ...
Detecting transposon-induced genomic variants using low
... and did not generated false positive calls, whatever the coverage. This result strengthens the reliability of LoRTE, even in a context of low coverage PacBio datasets. We finally analyzed the results obtained by LoRTE on genuine D. melanogaster PacBio reads. Fig 1C shows the number of deletion/inser ...
... and did not generated false positive calls, whatever the coverage. This result strengthens the reliability of LoRTE, even in a context of low coverage PacBio datasets. We finally analyzed the results obtained by LoRTE on genuine D. melanogaster PacBio reads. Fig 1C shows the number of deletion/inser ...
Analyzing the Changes in DNA Flexibility Due to Base Modifications
... chloride ions to achieve a 1 M concentration. The new PSF and PDB files will be written to dna WI.psf and dna WI.pdb files. ...
... chloride ions to achieve a 1 M concentration. The new PSF and PDB files will be written to dna WI.psf and dna WI.pdb files. ...
Optimizing Data Acquisition for Automated de novo Sequencing
... (fragmentation and detection) were obtained as detailed in Figure 2 (inset). The database search (BioWorks™ 3.3.1) was performed against the Uniprot-SwissProt database (containing 216,381 entries), considering fully tryptic peptides and carboxyamidation on Cys (+ 58.0055 Da) as a fixed modification. ...
... (fragmentation and detection) were obtained as detailed in Figure 2 (inset). The database search (BioWorks™ 3.3.1) was performed against the Uniprot-SwissProt database (containing 216,381 entries), considering fully tryptic peptides and carboxyamidation on Cys (+ 58.0055 Da) as a fixed modification. ...
Datasheet for Q5® Hot Start High-Fidelity 2X Master Mix
... patents in other countries for use only in: (a) standard (non-real time) PCR in the research field only, but not real-time PCR or digital PCR; (b) any in-vitro diagnostics application, except for applications using realtime or digital PCR; and (c) any non-PCR applications in DNA sequencing, isotherm ...
... patents in other countries for use only in: (a) standard (non-real time) PCR in the research field only, but not real-time PCR or digital PCR; (b) any in-vitro diagnostics application, except for applications using realtime or digital PCR; and (c) any non-PCR applications in DNA sequencing, isotherm ...
DNA structure 2008
... developed by F. Sanger • Base-specific chemical cleavage, developed by Maxam and Gilbert • Both use autoradiography - X-ray film develops in response to presence of radioactive isotopes in nucleic acid molecules Garrett and Grisham, Biochemistry, Third Edition ...
... developed by F. Sanger • Base-specific chemical cleavage, developed by Maxam and Gilbert • Both use autoradiography - X-ray film develops in response to presence of radioactive isotopes in nucleic acid molecules Garrett and Grisham, Biochemistry, Third Edition ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.