Unit 7 (Molecular Biology - DNA) Study Guide KEY
... a. Where are they located? mRNA b. How many bases are they made of? 3 c. What do they “code” for? amino acid (monomer of protein) d. Using the answer given in part c, how many of they does each codon “code” for? 1 20. What is an anticodon? 3 bases on tRNA that are complementary to codon on mRNA 21. ...
... a. Where are they located? mRNA b. How many bases are they made of? 3 c. What do they “code” for? amino acid (monomer of protein) d. Using the answer given in part c, how many of they does each codon “code” for? 1 20. What is an anticodon? 3 bases on tRNA that are complementary to codon on mRNA 21. ...
3. Sequence preprocessing
... – some replace Ns with random base, some with fixed base (e.g. SHAHA2 & Velvet = A) ...
... – some replace Ns with random base, some with fixed base (e.g. SHAHA2 & Velvet = A) ...
What is DNA? - mrgscience.com
... Part III. Learning About DNA Replication DNA can replicate itself. In this way, the hereditary information encoded in its structure is passed on to new cells formed by mitosis. During replication, the DNA double helix untwists, and the bonds between the nitrogen bases of each rung break. Nucleotide ...
... Part III. Learning About DNA Replication DNA can replicate itself. In this way, the hereditary information encoded in its structure is passed on to new cells formed by mitosis. During replication, the DNA double helix untwists, and the bonds between the nitrogen bases of each rung break. Nucleotide ...
Plasmid w/ kanamycin resistance (pKAN)
... 8,000bp 6,000bp 5,000bp 4,000bp 3,000bp 2,000bp 1,500bp 1,000bp 500bp ...
... 8,000bp 6,000bp 5,000bp 4,000bp 3,000bp 2,000bp 1,500bp 1,000bp 500bp ...
Unit 4: DNA: Our Genetic Material Notes
... 1. Rosalind Franklin used X-ray diffraction to get information about the structure of DNA. 2. She aimed an X-ray beam at concentrated DNA samples and recorded the scattering pattern of the X-rays on film. 3. Using clues from Franklin’s pattern, James Watson and Francis Crick built a model that expla ...
... 1. Rosalind Franklin used X-ray diffraction to get information about the structure of DNA. 2. She aimed an X-ray beam at concentrated DNA samples and recorded the scattering pattern of the X-rays on film. 3. Using clues from Franklin’s pattern, James Watson and Francis Crick built a model that expla ...
PCR of Scallop/pGEM Ligated DNA I. Introduction: A PCR reaction is
... This is then followed by a 72 C extension for 7 minutes and storage in the freezer until the following week. 5. The following week, come to lab first, and prepare a 2% TBE agarose gel with a 12-tooth comb as previously described. 6. To the two PCR samples add 2.5 µL of the 10X sample dilutor. Mix ge ...
... This is then followed by a 72 C extension for 7 minutes and storage in the freezer until the following week. 5. The following week, come to lab first, and prepare a 2% TBE agarose gel with a 12-tooth comb as previously described. 6. To the two PCR samples add 2.5 µL of the 10X sample dilutor. Mix ge ...
Assembly of complete KIR haplotypes from a diploid individual
... simplifies the assembly problem such that it is possible to unambiguously assemble individual haplotypes for the highly repetitive 100-200 kb killer Ig-like receptor (KIR) gene loci of chromosome 19. A tiling of targeted fosmids can be used to clone extended lengths of genomic DNA, 100s of kb in len ...
... simplifies the assembly problem such that it is possible to unambiguously assemble individual haplotypes for the highly repetitive 100-200 kb killer Ig-like receptor (KIR) gene loci of chromosome 19. A tiling of targeted fosmids can be used to clone extended lengths of genomic DNA, 100s of kb in len ...
DNA
... – Is the hereditary material in viruses protein or DNA? B. PROCEDURE 1. Label protein and DNA in a phage with radioactive isotopes (S-35 and P-32) ...
... – Is the hereditary material in viruses protein or DNA? B. PROCEDURE 1. Label protein and DNA in a phage with radioactive isotopes (S-35 and P-32) ...
BIOL212aluIntronLabSpr2012
... In Your Notebook: This first week in lab is a great time to develop good lab notebook skills! Your notebook is your record of what you did and observed during your time in lab. (Please consult your instructor and/or handout for guidelines on general lab notebook requirements and format.) As always, ...
... In Your Notebook: This first week in lab is a great time to develop good lab notebook skills! Your notebook is your record of what you did and observed during your time in lab. (Please consult your instructor and/or handout for guidelines on general lab notebook requirements and format.) As always, ...
For the last three and a half billion years, evolution has been
... http://www3.oup.co.uk/nar/database/c/ ...
... http://www3.oup.co.uk/nar/database/c/ ...
Plasmid w/ kanamycin resistance (pKAN)
... • Obtain the plasmids (pKAN and pAMP) P stands for plasmid pKAN = plasmid with antibiotic kanamycin resistance pAMP = plasmid with antibiotic ampicillin resistance ...
... • Obtain the plasmids (pKAN and pAMP) P stands for plasmid pKAN = plasmid with antibiotic kanamycin resistance pAMP = plasmid with antibiotic ampicillin resistance ...
plasmid to transform
... 3. Prepare bacterial cells for transformation of plasmid. a. Treat with calcium chloride, which allows plasmid to pass through bacterial cell walls. This is the most common ...
... 3. Prepare bacterial cells for transformation of plasmid. a. Treat with calcium chloride, which allows plasmid to pass through bacterial cell walls. This is the most common ...
DNA in a Bottle
... - The'DNA'from'a'single'human'put'end'to'end'could'travel'to'the'sun'and' back'over'600'times'(moon'and'back'6000'times)' - Typing'at'60'words'per'minute,'it'would'take'~50'years'to'type'the' human'genome' - Errors'are'caused'by'alterations'to'a'human’s'DNA'about'1000'times'a' day' - DNA'was'discove ...
... - The'DNA'from'a'single'human'put'end'to'end'could'travel'to'the'sun'and' back'over'600'times'(moon'and'back'6000'times)' - Typing'at'60'words'per'minute,'it'would'take'~50'years'to'type'the' human'genome' - Errors'are'caused'by'alterations'to'a'human’s'DNA'about'1000'times'a' day' - DNA'was'discove ...
14–16 Video transcript: Chickens and Campylobacter
... So what happens next when the material's back at the lab is that we isolate and grow the Campylobacter. Then we boil the bacterial cells to extract the DNA, and then we sequence the DNA and this means that we compare the isolates we've collected this morning with a whole database full of isolates th ...
... So what happens next when the material's back at the lab is that we isolate and grow the Campylobacter. Then we boil the bacterial cells to extract the DNA, and then we sequence the DNA and this means that we compare the isolates we've collected this morning with a whole database full of isolates th ...
Source Identification of Body Fluid Stains Using DNA
... necessary to consider the probability that a relative of a suspect may have the same profile. If it is not possible to obtain known standards from pertinent siblings or other relatives, the conditional probability, p', can be calculated using formulae described in the NRC II report (2, page 113). Th ...
... necessary to consider the probability that a relative of a suspect may have the same profile. If it is not possible to obtain known standards from pertinent siblings or other relatives, the conditional probability, p', can be calculated using formulae described in the NRC II report (2, page 113). Th ...
Next-Generation Sequencing: an overview of technologies and
... – Sequencing by synthesis – Sequencing by ligation • Mostly produce short reads- from <400bp • Read numbers vary from ~ 1 million to ~ 1 billion per run ...
... – Sequencing by synthesis – Sequencing by ligation • Mostly produce short reads- from <400bp • Read numbers vary from ~ 1 million to ~ 1 billion per run ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.