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The ability to isolate plasmid DNA is crucial to recombinant DNA
The ability to isolate plasmid DNA is crucial to recombinant DNA

... NaOH (sodium hydroxide – a base). In addition to lysing the cells, these components serve several other valuable functions. The SDS and the NaOH solubilize and denature cellular constituents, and the elevated pH will begin to degrade RNA. The alkaline conditions also result in DNA denaturation (stra ...
Activity
Activity

... -Students will use paper and pencil to replicate DNA using a known sequence from the globin gene. -Students will determine which enzymes are necessary for replication, which direction replication occurs in, which bases are complementary and learn the meaning of the terms: semi-conservative replicati ...
DNA PowerPoint
DNA PowerPoint

... Each new DNA molecule has one new stand and one strand from the original molecule. The enzyme DNA polymerase, the principal enzyme, “proofreads” the new DNA strands, helping to maximize the odds that each molecule is a perfect copy of the original. ...
DNA RNA Review - OG
DNA RNA Review - OG

... molecules, each containing 1 “original” strand, and 1 “new” strand ...
Chapter 16 DNA
Chapter 16 DNA

... intermediate Cells grown longer 14N, make lighter DNA ...
DNA History, Structure, and Replication – Part 2
DNA History, Structure, and Replication – Part 2

... 3. The radioactive viruses where then exposed to bacteria. The viruses infected the bacteria. In the radioactive Sulfur container, the radioactive sulfur did NOT enter the bacteria. It remained outside the bacteria. When the viruses reproduced inside the bacteria, the reproduced viruses that came ou ...
09-DNA-Replication
09-DNA-Replication

...  The work of Sutton and Morgan established that genes reside on chromosomes  But chromosomes contain proteins and DNA  So which one is the hereditary material  Several experiments ultimately revealed the nature of the genetic material ...
Chapter Worksheet
Chapter Worksheet

... If the statement is true, write true. If the statement is false, replace the italicized term or phrase to make it true. 1. Gene regulation is the ability of an organism to control which genes are transcribed. ...
Discovering DNA: Structure and Replication
Discovering DNA: Structure and Replication

... Monomer of a nucleic acid is a nucleotide The three parts of a nucleotide: – 1. Phosphate group – 2. Sugar (deoxyribose) – 3. Nitrogen base ...
answer key
answer key

... Because they run in opposite directions to each other without crossing (ie: one points from 3’  5’, and the other points from 5’  3’). 7. Describe the process of DNA replication in a cell. Include all enzymes and components involved, and be sure to indicate what is produced at the end of this proc ...
Genes- PRACTICE PROBLEMS- ANSWERS
Genes- PRACTICE PROBLEMS- ANSWERS

... 71. The ribosome moves the mRNA using energy and new amino acids are continually added by new tRNA molecules. Each new tRNA moves from the A site to the P site and then leaves the ribosome. 72. Termination occurs when the ribosome reaches a stop codon. The ribosome subunits separate from the mRNA an ...
Chapter 4.1 Notes: “DNA: The Molecule of Life”
Chapter 4.1 Notes: “DNA: The Molecule of Life”

... Write the nucleotide base sequence that complements the following DNA sequence: A T G C C A T G C --------> TAC GGT ACG ...
I. DNA - Humble ISD
I. DNA - Humble ISD

... _Deoxyribonucleic acid_______ contains the _genetic code____ and the _working instructions______ for a cell. In a _eukaryotic___ cell, DNA is located in the _nucleus___. Although _prokaryotic____ cells do not contain a nucleus, they do contain a _single__ molecule of DNA, or one _chromosome_________ ...
Biol120 Mock Final Examination
Biol120 Mock Final Examination

... 16. Based on his experiments with pea plants, Mendel found he was able to predict that: a) Half of the offspring will have the same genotype as one of their parents b) As gametes are formed half the gametes carry one allele, and the other half carry the other allele c) The alleles of the genes that ...
DNA/RNA/Transcription/Translation Notes DNA, RNA, Replication
DNA/RNA/Transcription/Translation Notes DNA, RNA, Replication

... ● Synthesized from DNA during transcription. ● Contains codons that code for amino acids. rRNA- make up the ribosomes. tRNA ● Transfers amino acid to ribosome during translation. ● Contain an anticodon that complement the codon on mRNA. ...
CHAPTER 4 Notes, Part 2: FLOW OF GENETIC INFORMATION Fall
CHAPTER 4 Notes, Part 2: FLOW OF GENETIC INFORMATION Fall

... GC-rich region followed by a string of U's which can form a base-paired hairpin loop. (Fig. 5.28) ...
DNA ‐ The Double Helix
DNA ‐ The Double Helix

... Messenger
RNA
is
similar
to
DNA,
except
that
it
is
a
single
strand,
and
it
has
no
thymine.

Instead
of
 thymine,
mRNA
contains
the
base
Uracil.

In
addition
to
that
difference,
mRNA
has
the
sugar
ribose
 instead
of
deoxyribose.

Thus,
RNA
stands
for
Ribonucleic
Acid.
 ...
Biology: DNA Unit Review
Biology: DNA Unit Review

... 20. Which mRNA codon will start the process of translation?________________________________________ 21. Which amino acid does every protein begin with?______________________________________________ 22. Which mRNA codons will end the process of translation?________________________________________ 23. ...
Nucleic acids and protein synthesis
Nucleic acids and protein synthesis

... expression in eukaryotes.  During RNAi, microRNAs (miRNAs) bind to transcribed mRNA to block it from being translated into protein. ...
Slide 1
Slide 1

... The nucleosome is composed of an octameric core of histone proteins around which the DNA double helix is wrapped. Nucleosomes are usually packed together (with the aid of histone H1 molecules) into quasi-regular arrays to form a 30-nm fiber. Despite the high degree of compaction in chromatin, its st ...
TAKS Objective 2: The student knows the structures and functions of
TAKS Objective 2: The student knows the structures and functions of

... Students will turn in extra credit to their science teacher. The requirements and format is found on the attachment. Some slides consist of activities that the students will perform. ...
Date: Monday/Tuesday, March
Date: Monday/Tuesday, March

... a. mRNA  exits  the  nucleus   b. DNA  is  “unzipped”  by  special  enzymes  in  the  nucleus   c. DNA  re-­‐winds  into  a  double  helix   d. mRNA  is  created  by  complimentary  base  pairing  to  a  single  DNA  strand   4. The  fi ...
Document
Document

... acid produced by a codon because of redundancy in the genetic code • Missense mutations still code for an amino acid, but not the correct amino acid • Nonsense mutations change an amino acid codon into a stop codon, nearly always leading to a nonfunctional protein © 2011 Pearson Education, Inc. ...
DNA - Weebly
DNA - Weebly

... proteins by the arrangement of nitrogenous bases. • The code for a particular amino acid (the base unit of proteins) is determined by a sequence of three base pairs on the DNA molecule. • A gene is a specific location on a chromosome, consisting of a segment of DNA, that codes for a particular prote ...
Inquiry: How is DNA used to store and transmit cell information?
Inquiry: How is DNA used to store and transmit cell information?

... DNA Replication • DNA is unique among all known molecules because it is the only one that is capable of duplicating itself. • The process of duplication is called replication. • During replication, the two complementary strands which form the DNA molecule unzip and then are used as templates from w ...
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Helicase



Helicases are a class of enzymes vital to all living organisms. Their main function is to unpackage an organism's genes. They are motor proteins that move directionally along a nucleic acid phosphodiester backbone, separating two annealed nucleic acid strands (i.e., DNA, RNA, or RNA-DNA hybrid) using energy derived from ATP hydrolysis. There are many helicases resulting from the great variety of processes in which strand separation must be catalyzed. Approximately 1% of eukaryotic genes code for helicases. The human genome codes for 95 non-redundant helicases: 64 RNA helicases and 31 DNA helicases. Many cellular processes, such as DNA replication, transcription, translation, recombination, DNA repair, and ribosome biogenesis involve the separation of nucleic acid strands that necessitates the use of helicases.
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