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... 2. Why does Adenine bond with Thymine and not with Cytosine? 3. Weak hydrogen bonds hold the DNA helix together. What is the importance of using weak bonds? 4. If 15% of the nucleotides in DNA are cytosine, what percentage of the nucleotides are adenine? Why? 5. What types of bonds exist in DNA and ...
Answered Review Questions The Recipe of Life 1. Describe the
Answered Review Questions The Recipe of Life 1. Describe the

... Streptococcus pneumoniae bacteria. The cells of the “smooth” strain (S) are encapsulated and cause a lethal case of pneumonia. The “rough” strain (R) lack a capsule and are harmless. Mice injected with S cells die. Mice injected with R cells live. Mice injected with S cells that have been heat-kille ...
Protein Synthesis Powerpoint
Protein Synthesis Powerpoint

... • The anticodon of an aa-tRNA molecule binds to the mRNA codon exposed in the A site. • Enzymes catalyze the formation of a bond between the last aa on the lengthening polypeptide and the new aa. The polypeptide chain is transferred from the tRNA in the P site to the tRNA in the A site. • The ribos ...
Ch. 12.2: Replication of DNA
Ch. 12.2: Replication of DNA

... Replication of DNA • Before a cell can divide by mitosis or meiosis, it must first make a copy of its chromosomes. • The DNA in the chromosomes is copied in a process called DNA replication. • Without DNA replication, new cells would have only half the DNA of their parents. • DNA is copied during in ...
How many tetrads are there in metaphase I of
How many tetrads are there in metaphase I of

... 5. Which of the following options would result from the actions of DNA polymerase during DNA replication? A. Two DNA polymerase molecules act to synthesize a short segment of daughter DNA strand from each parental strand; ligase is used to connect these short segments of both daughter strands. B. Tw ...
Julia Louise Harris Behnfeldt Dissertation Defense Booklet
Julia Louise Harris Behnfeldt Dissertation Defense Booklet

... Genomic instability, including chromosome breakage, can arise from dysfunctional cell cycle control, environmental agents that damage DNA or ineffective DNA repair; it’s a hallmark of most cancers. The induction or regulation of genomic instability also represents an opportunity in cancer therapeuti ...
DNA Replication and Protein Synthesis PowerPoint
DNA Replication and Protein Synthesis PowerPoint

... • Transcription - Initiation ✓ RNA polymerase binds to a region on DNA known as the promoter, which signals the start of a gene (does not need a primer) ✓ Promoters are specific to genes ■ TATA Box ✓ Transcription factors assemble at the promoter forming a transcription initiation complex – activato ...
Study guide
Study guide

... Favorite music, dyeing hair, choice of clothing style, language spoken • Why do identical twins have the same DNA and not the same personality? Personality is not a result of DNA (inherited traits), but environmental ones instead ...
DNA Replication Simulation WKST
DNA Replication Simulation WKST

... As you pulled the strands apart, you may have noticed the ends were still wound around each other. If there was nothing to release this tension, it would continue to tangle up the DNA. To demonstrate this, pull on two origins of replication on your string and notice what happens between the replica ...
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DNA - Faperta UGM

... Gadjah Mada University ...
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RNA

... explain how a gene works. • DNA contains coded instructions for making proteins. • The first step of making proteins is decoding the genetic messages by copying the nucleotide sequence from DNA into RNA. ...
DNA REPLICATION Complexity of DNA
DNA REPLICATION Complexity of DNA

... Topoisomerase II - cleaves both strands, allows unwinding of the overwound Watson and Crick strands (an energy-requiring process) and rejoins the ends. Uses ATP as a co-factor. A version of this enzyme in bacteria is called "gyrase." Gyrase makes a double strand scission, forcibly underwinds the DNA ...
Document
Document

... Griffith: bacterial work; transformation: change in genotype and phenotype due to assimilation of external substance (DNA) by a cell Avery: transformation agent was DNA ...
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chp-5 nucleic acid

... – Renaturation/annealing is possible ...
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DNA notes 2014

... signifies that the first two species must be __________________ _________________________________________ to each other than to the other species ...
DNA! - Chapter 10
DNA! - Chapter 10

... 8. During translation, what amino acid sequence would the following mRNA segment be converted into: AUGGACAUUGAACCG? 9. How come there are only 20 amino acids when there are 64 different codons? 10. How come prokaryotes can both transcribe and translate a gene at the same time, but eukaryotes cannot ...
DNA - Science-with
DNA - Science-with

...  in DNA  the amount of adenine is always approximately equal to the amount of thymine (A ~ T)  the amount of cytosine is always approximately equal to the amount guanine. (C ~ G) ...
„DNA damage“?
„DNA damage“?

... • the MMR contributes to replication fidelity by a factor of 103 by removal of base-base mismatches, insertions and deletions (hence the resulting incidence of mutations due to erroneous replication is only 1:1010) • the system must be able discrimitate between parental and daughter DNA strand! • Mu ...
DNA HISTORY NOTES
DNA HISTORY NOTES

... Monomer of a nucleic acid is a nucleotide The three parts of a nucleotide: – 1. Phosphate group – 2. Sugar (deoxyribose) – 3. Nitrogen base ...
DNA Ligase
DNA Ligase

... Helicase: unwinds DNA at origins of replication Initiation proteins separate 2 strands  forms replication bubble Primase: puts down RNA primer to start replication DNA polymerase III: adds complimentary bases to leading strand (new DNA is made 5’  3’) 5. Lagging strand grows in 3’5’ direction by ...
DNA Replication Complex
DNA Replication Complex

... tend to be shorter in cells that have divided multiple times. In cultured cells or somatic cells of an older organism telomeres will likely be shorter. In eukaryotic germ cells, there is an enzyme known as telomerase which lengthens the telomeres and restoring them to their original length to compen ...
Chapter 16 DNA
Chapter 16 DNA

... Helicase: unwinds DNA at origins of replication Initiation proteins separate 2 strands  forms replication bubble Primase: puts down RNA primer to start replication DNA polymerase III: adds complimentary bases to leading strand (new DNA is made 5’  3’) 5. Lagging strand grows in 3’5’ direction by ...
Chapter16ppt
Chapter16ppt

... Helicase: unwinds DNA at origins of replication Initiation proteins separate 2 strands  forms replication bubble Primase: puts down RNA primer to start replication DNA polymerase III: can only add to 3’ end of growing strand adds complimentary bases to leading strand (new DNA is made ...
DNA replication
DNA replication

... • Each human cell contains 46 chromosomes (6 to 9 feet of DNA) • Your body contains 75-100 trillion of cells. • All of your DNA (when uncoiled and tied ...
DNA replication
DNA replication

... • Each human cell contains 46 chromosomes (6 to 9 feet of DNA) • Your body contains 75-100 trillion of cells. • All of your DNA (when uncoiled and tied ...
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Helicase



Helicases are a class of enzymes vital to all living organisms. Their main function is to unpackage an organism's genes. They are motor proteins that move directionally along a nucleic acid phosphodiester backbone, separating two annealed nucleic acid strands (i.e., DNA, RNA, or RNA-DNA hybrid) using energy derived from ATP hydrolysis. There are many helicases resulting from the great variety of processes in which strand separation must be catalyzed. Approximately 1% of eukaryotic genes code for helicases. The human genome codes for 95 non-redundant helicases: 64 RNA helicases and 31 DNA helicases. Many cellular processes, such as DNA replication, transcription, translation, recombination, DNA repair, and ribosome biogenesis involve the separation of nucleic acid strands that necessitates the use of helicases.
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