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GLOSSARY Adenine : (6-aminopurine)
GLOSSARY Adenine : (6-aminopurine)

... DNA supercoiling: The coiling of DNA upon itself, as a result of bending, under-winding or overwinding of the DNA helix. ...
Concept 18.3. How get genetic variation in prokaryotes: • E. coli is
Concept 18.3. How get genetic variation in prokaryotes: • E. coli is

... E. coli is the lab rat of molecular biology. DNA is ds, circular and associated with proteins = 1mm length. Eukaryotic DNA is linear and associated with lots of proteins. 4.6 million bases = 4,400 genes, 1/1000th DNA in Human somatic cells. DNA fills nucleoid-dense region of DNA. In addition have pl ...
View PDF
View PDF

... Eukaryotic DNA is linear and associated with lots of proteins. 4.6 million bases = 4,400 genes, 1/1000th DNA in Human somatic cells. DNA fills nucleoid-dense region of DNA. In addition have plasmids ( several dozen genes). Divide by binary fission. Fig. 18.14 Replication of Bacterial DNA-single orig ...
Name
Name

... 2.What is rDNA 3. What is a vector? 4.Define the following terms: ...
Lab 1: Split Pea DNA Extraction Questions to consider Where is
Lab 1: Split Pea DNA Extraction Questions to consider Where is

... What is the white stringy stuff? DNA is a long, stringy molecule. The salt that you added in step one helps it stick together. So what you see are clumps of tangled DNA molecules! DNA normally stays dissolved in water, but when salty DNA comes in contact with alcohol it becomes undissolved. This is ...
Arrays
Arrays

... Doctors will soon love DNA chips • Researchers love DNA chips because they give a huge amount of information, fast, at low cost. • Doctors will soon learn to love them because there are many times when a doctor would like to know something about a patient's genes (such as whether the patient is like ...
File
File

... How do nitrogenous bases bind to each other? Which part of a nucleotide makes up the actual “code” that DNA is said to contain? Who were the two men who discovered the structure of DNA? What did Rosalind Franklin do that greatly helped the men in question 4 prove that they had discovered the structu ...
DNA Analysis of Various Mouse Organs
DNA Analysis of Various Mouse Organs

... • Gel electrophoresis allowed for visualization of DNA from the varying organ tissues. ...
DNA Review Packet
DNA Review Packet

... DNA Review Packet DNA Notes to study: DNA Structure, DNA Replication, Protein Synthesis, and Mutations. Read each question and fill in the proper answer. 1. Label EVERY sugar (S), phosphate (P), and nitrogen base (A, T, C, G) in the diagram below. ...
Nucleic Acids and DNA Replication
Nucleic Acids and DNA Replication

... • Chargraff’s Rule: for any given species the % of Ts will by equivalent of the % of As while the % of Cs will be equivalent to the % of Gs ...
DNA Extraction Lab
DNA Extraction Lab

... Mashed Strawberry Filtered Strawberry Strawberry with Extraction Solution Strawberry with Isopropyl Alcohol DNA ...
I - cloudfront.net
I - cloudfront.net

... Take the tour of DNA by clicking on “What is DNA?” and answer the questions below: 1. In what organelle (CELL PART) would I find your DNA (YOUR INSTRUCTIONS)? _________________ 2. What does DNA stand for? __________________________ 3. The DNA molecule comes in the form of a ___________ladder and sci ...
2/4:DNA extraction lab
2/4:DNA extraction lab

... membranes. Cell membranes and nuclear membranes consist primarily of lipids. Dishwashing detergent, like all soaps, breaks up clumps of lipids. This is why you use detergents to remove fats. Why did I add enzymes? The nucleus of each of your cells contains multiple long strands of DNA with all the i ...
DNA REPLICATION Review of DNA Structure
DNA REPLICATION Review of DNA Structure

... RNA primer complementary to the DNA templates • After formation of the primer, DNA polymerase III – elongates the new strand by adding nucleotides to the 3’end (~50 per ...
1 kb ladder.eng Ed.08. March 14
1 kb ladder.eng Ed.08. March 14

... 5- Visualise DNA by staining with ethidium bromide or with SYBR® Green I. *The mixture should be scaled up or down, depending on the width of the agarose gel. Use 0.1µg of DNA ladder/mm of lane. The 1kb DNA Ladder was not designed for precise quantification of DNA mass, but can be used for semi-quan ...
Molecules of Life
Molecules of Life

... • This protein synthesis is carried out by ribosomes. ...
DNA Replication - Blue Valley Schools
DNA Replication - Blue Valley Schools

... • Base pairs are formed by the hydrogen bonding of A with T and G with C. • This pattern is constant for all organisms. • The sequence of bases in a nucleotide strand is different from one species to the next. ...
DNA
DNA

... connected chains of nucleotides wound into a double helix • Double – two strands Helix- spiral • Looks like a twisted ladder ...
Dining On Delicious DNA Handout
Dining On Delicious DNA Handout

... 2. Crush strawberry inside bottom of bag by pressing on it with your fingers. 3. Add 10mL of the extraction buffer you made at the beginning. 4. Continue pressing the strawberry, mixing it with the buffer solution. 5. Assemble a filtration device over an empty test tube. Cut cheesecloth to fit over ...
DNA
DNA

... ways to form an endless amount words • In the same way, the 4 bases can be combined in endless different ways to form an endless amount of different sequences, resulting in different genes ...
Coding Exercises Worksheet
Coding Exercises Worksheet

... tRNA Mutations occur when there is a spelling error in the DNA code. Show what would happen step by step if the 8th letter in the DNA strand from above was changed to a “G”. Use the chart at the top of the page to determine the amino acid coded for by mRNA DNA: T A C C C A A G T C G T A A C T G C G ...
File
File

... organisms or their components for the purpose of making useful products. ...
The genetic engineers toolkit
The genetic engineers toolkit

... They leave blunt or sticky ends. There are over 400 restriction enzymes so genetic engineers can cut DNA almost any where they want. they can cut out specific genes using restriction enzymes. ...
C503
C503

The Biology Behind DNA Fingerprinting
The Biology Behind DNA Fingerprinting

... As an example, let’s say that in a population of 1000 people, 650 people have the AA genotype, 300 have the Aa genotype, and 50 have the aa genotype. The frequency of the A allele is determined by summing the number of A alleles in the population and then dividing by 2 times the total. ...
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DNA repair



DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.
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