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Learning Targets
Learning Targets

... 11. What are the roles of the DNA, mRNA, tRNA, rRNA, and ribosomes in protein synthesis? 12. What are the steps of the transcription phase of protein synthesis? 13. What are the steps of the translation phase of protein synthesis, and why is translation important? 14. Given the DNA codes for various ...
Chapter 9
Chapter 9

... • Amount of adenine always equals amount of thymine, and amount of guanine always equals amount of cytosine A=T and G=C ...
Bio07_TR__U04_CH12.QXD
Bio07_TR__U04_CH12.QXD

... 21. Is the following sentence true or false? Adenine and guanine are larger molecules than cytosine and thymine because they have two rings in their structure. 22. What forms the backbone of a DNA chain? 23. Is the following sentence true or false? The nucleotides must be joined together in a specif ...
NoLimits 1000bp DNA Fragment
NoLimits 1000bp DNA Fragment

... The NoLimits™ DNA Fragment is a chromatographypurified, exceptionally pure individual DNA fragment. It is produced using specifically designed plasmid DNA purified by a proprietary patent-pending technology. ...
Biocatalysis - Chatham University
Biocatalysis - Chatham University

... ESSENTIALS STEPS IN DNA CLONING 1. Cutting target DNA at precise locations. Sequence-specific endonucleases (restriction endonucleases) provide the necessary molecular scissors 2. Selecting a small carrier molecule of DNA capable of selfreplication. These DNAs are called cloning vectors (typically ...
肺癌和乳房癌病人DNA修复能力及关卡基因蛋白 (ATM蛋白
肺癌和乳房癌病人DNA修复能力及关卡基因蛋白 (ATM蛋白

... chromosomal instability is critical for effective diagnostic and prognostic evaluation and therapeutic intervention of cancer. ...
The Double Helix video notes
The Double Helix video notes

... a) What was the other type of molecule that some scientists thought might carry genetic information? _____________________________ b) Why did this other type of molecule seem like a likely candidate? ____________________________________________________________________________________________________ ...
less ID: genetic engineering
less ID: genetic engineering

... 2. Hybrids are often hardier t h a ~either of their parents. ...
Name
Name

... nucleotides in its structure. 5. RNA molecules have _sugar/phosphate (ribose) backbone. 6. DNA uses the sugar _deoxyribose___________ 7. DNA uses the nitrogenous bases adenine, thymine, guanine and cytosine__________ 8. One DNA molecule can include _4________ different nucleotides in its structure. ...
Recombinant DNA Technology
Recombinant DNA Technology

... bp from the binding site • Type III – cleave about 25 b ffrom the ...
Restriction Mapping Restriction Fragment Length Polymorphism
Restriction Mapping Restriction Fragment Length Polymorphism

... by one enzyme contains restriction sites for the other enzyme. Fragments are aligned by size. ...
Key for Practice Exam 4
Key for Practice Exam 4

DNA Structure and Replication
DNA Structure and Replication

... 3. How long does it take for your cells to replicate your DNA? 4. When DNA is replicated during the cell cycle? 5. What is the average rate of DNA replication? 6. How does DNA speed up the process of replication? 7. How are the bases of the nucleotides held together? DNA REPLICATION http://www.hhmi. ...
NAME ______ANSWER KEY CH. 8 HONORS STUDY GUIDE
NAME ______ANSWER KEY CH. 8 HONORS STUDY GUIDE

... 3. During what part of the cell cycle will replication take place & how many times? A: ONCE, S PHASE OF INTERPHASE 4. After replication, explain how the new & old strands of DNA are arranged. A: ONE OLD STRAND IS ATTACHED TO ONE NEW STRAND 5. What is the function of the enzyme DNA polymerase? A: TO ...
Unit 8 Objectives and Vocab L4
Unit 8 Objectives and Vocab L4

... 3. Describe the structure of DNA and explain what kind of chemical bond connects the nucleotides of each strand and what holds the two strands together. 4. Describe the process of DNA replication and explain the role of helicase, primase, DNA polymerase, ligase, leading and lagging strands. 5. Descr ...
Name Date Period BioTechnology: Web Quest Part 1
Name Date Period BioTechnology: Web Quest Part 1

... Read the introduction and the 3 main points of gel electrophoresis. Run the animations. 1. The DNA is being cut into fragments by __________________________________ 2. Where are the fragments transferred? ___________________________________ 3. What goes through the gel that creates a negative charge ...
DNA Notes
DNA Notes

... - When a cell is not dividing, DNA is in the form of chromatin. - Loosely packed DNA that is wrapped around proteins (called histones) ...
No Slide Title
No Slide Title

... The restriction enzyme Eco.R1 found in strain C, E. coli bacteria They don’t make straight cuts, but produce sticky ends These sticky ends can rejoin by forming hydrogen bonds and the sugar-phosphates rejoining with the help of the enzyme ligase The DNA produced by restriction enzymes cutting is cal ...
NAME: :__________PERIOD:____ Cell Structure, DNA Structure
NAME: :__________PERIOD:____ Cell Structure, DNA Structure

... d.) proteins ...
Guidelines and Assignments
Guidelines and Assignments

... 1. (MT1) A. How is the 5-mC distributed within the human genome? B. Do all human genes have CpG island at their promoters? C. How bisulfite treatment may affect the CpG methylation status? D. What methods can be used to detect the methylation status of DNA? Please describe at least four different me ...
242413_Fx_DNA_Fingerprinting_Lab
242413_Fx_DNA_Fingerprinting_Lab

11.2 DNA and reproduction
11.2 DNA and reproduction

Protein Synthesis Reading
Protein Synthesis Reading

... coded letters --- A, G, T, and C. These bases always bond in a certain way. Adenine will only bond to thymine. Guanine will only bond with cytosine. This is known as the "Base-Pair Rule." The bases can occur in any order along a strand of DNA. The order of these bases is the code that contains the i ...
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... DNA - The Double Helix Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. How does it do this? The nucleus controls these activities with chromosomes ...
DNA Sequencing
DNA Sequencing

... 1. Aliquot A + dimethyl sulphate, which methylates guanine residue 2. Aliquot B + formic acid, which modifies adenine and guanine residues 3. Aliquot C + Hydrazine, which modifies thymine + cytosine residues 4. Aliquot D + Hydrazine + 5 mol/l NaCl, which makes the reaction specific for cytosine ...
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DNA repair



DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.
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