Review for Lecture 18
... 7. This continues on to Southern blotting – how does this technique work? How would you set it up? What is the purpose? See example of how it is used in DNA fingerprinting. 8. Understand how dideoxy sequencing is done – the use of dideoxynucleotides to create fragments of DNA of different lengths. H ...
... 7. This continues on to Southern blotting – how does this technique work? How would you set it up? What is the purpose? See example of how it is used in DNA fingerprinting. 8. Understand how dideoxy sequencing is done – the use of dideoxynucleotides to create fragments of DNA of different lengths. H ...
Why is DNA Replication
... attaches to the DNA and adds complimentary bases (A,T,C,G) to make new sides of DNA 3. Then, DNA Polymerase checks and edits the new side to make sure the proper nitrogen bases ...
... attaches to the DNA and adds complimentary bases (A,T,C,G) to make new sides of DNA 3. Then, DNA Polymerase checks and edits the new side to make sure the proper nitrogen bases ...
1. To research…
... DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where ...
... DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where ...
C1. Self-assembly occurs spontaneously, without the aid of other
... C12. The centromere is the attachment site for the kinetochore, which attaches to the spindle. If a chromosome is not attached to the spindle, it is free to “float around” within the cell, and it may not be near a pole when the nuclear membrane re-forms during telophase. If a chromosome is left outs ...
... C12. The centromere is the attachment site for the kinetochore, which attaches to the spindle. If a chromosome is not attached to the spindle, it is free to “float around” within the cell, and it may not be near a pole when the nuclear membrane re-forms during telophase. If a chromosome is left outs ...
Activity--Extracting DNA - e
... An enzyme is a chemical that promotes a reaction. In this case it breaks up the proteins that surround the DNA. 8. Tilt your test tube and slowly pour rubbing alcohol (70-95 percent isopropyl or ethyl alcohol) into the tube down the side so that it forms a layer on top of the pea mixture. Pour until ...
... An enzyme is a chemical that promotes a reaction. In this case it breaks up the proteins that surround the DNA. 8. Tilt your test tube and slowly pour rubbing alcohol (70-95 percent isopropyl or ethyl alcohol) into the tube down the side so that it forms a layer on top of the pea mixture. Pour until ...
Document
... C12. The centromere is the attachment site for the kinetochore, which attaches to the spindle. If a chromosome is not attached to the spindle, it is free to “float around” within the cell, and it may not be near a pole when the nuclear membrane re-forms during telophase. If a chromosome is left outs ...
... C12. The centromere is the attachment site for the kinetochore, which attaches to the spindle. If a chromosome is not attached to the spindle, it is free to “float around” within the cell, and it may not be near a pole when the nuclear membrane re-forms during telophase. If a chromosome is left outs ...
DNA History, Structure, and Replication – Part 2
... Alfred Hershey and Martha Chase (in 1952) A. They worked with the T2 Bacteriophage (a virus that infects bacteria) and E. Coli bacteria.( Fig:16.3) B. This becomes the Hershey-Chase Experiment. (Fig:16.4) 1. They used radioactive Sulfur 35 to label the virus’s protein outer capsid in one container. ...
... Alfred Hershey and Martha Chase (in 1952) A. They worked with the T2 Bacteriophage (a virus that infects bacteria) and E. Coli bacteria.( Fig:16.3) B. This becomes the Hershey-Chase Experiment. (Fig:16.4) 1. They used radioactive Sulfur 35 to label the virus’s protein outer capsid in one container. ...
No Slide Title
... A library is simply a collection of clones. Genomic clones are made from chromosomal DNA of some organism. A Genome Equivalent is the number of clones it would take for the size of the cloned fragments to equal the size of the genome of the organism. Fox example, consider a genome equivalent for mai ...
... A library is simply a collection of clones. Genomic clones are made from chromosomal DNA of some organism. A Genome Equivalent is the number of clones it would take for the size of the cloned fragments to equal the size of the genome of the organism. Fox example, consider a genome equivalent for mai ...
Organization and Control of Eukaryotic Genomes
... 1. Histones: + charged proteins (arginine/lysine) attract to – PO4 2. Nucleosomes: 4 histone protein structure around which is wrapped DNA with linker DNA between; linker variable-average 200 nucleotide pair repeat, 146 in nucleosome. ...
... 1. Histones: + charged proteins (arginine/lysine) attract to – PO4 2. Nucleosomes: 4 histone protein structure around which is wrapped DNA with linker DNA between; linker variable-average 200 nucleotide pair repeat, 146 in nucleosome. ...
Hotstart Taq DNA Polymerase
... HotStart Taq DNA polymerase is designed for quantitative PCR, a technique that enhances the specificity, sensitivity and yield of DNA amplification. HotStart Taq DNA polymerase is a recombinant Taq DNA polymerase which has been chemical mediated by the addition of heat-labile blocking groups to its ...
... HotStart Taq DNA polymerase is designed for quantitative PCR, a technique that enhances the specificity, sensitivity and yield of DNA amplification. HotStart Taq DNA polymerase is a recombinant Taq DNA polymerase which has been chemical mediated by the addition of heat-labile blocking groups to its ...
DNA and Chromosomes
... amount of genetic material so that when a cell splits, both will have the same amount of DNA as the original did. In bacteria, DNA replication is how cells reproduce. In human beings and other animals, other steps are required. This is called BINARY FISSION and it is a form a asexual reproductio ...
... amount of genetic material so that when a cell splits, both will have the same amount of DNA as the original did. In bacteria, DNA replication is how cells reproduce. In human beings and other animals, other steps are required. This is called BINARY FISSION and it is a form a asexual reproductio ...
DNA Sequencing:
... Note that the higher the concentration of the ddNTP in the reaction, the shorter the products will be, hence, you will get sequence CLOSER to your primer. With lower concentrations of ddNTP, chain termination will be less likely, and you will get longer products (sequence further AWAY from the prime ...
... Note that the higher the concentration of the ddNTP in the reaction, the shorter the products will be, hence, you will get sequence CLOSER to your primer. With lower concentrations of ddNTP, chain termination will be less likely, and you will get longer products (sequence further AWAY from the prime ...
Name Class Date DNA Replication Make Up #18 Lesson Objectives
... reconstruct the other half by the mechanism of base pairing. Because each strand can be used to make the other strand, the strands are said to be complementary. DNA copies itself through the process of replication: The two strands of the double helix unzip, forming replication forks. New bases are a ...
... reconstruct the other half by the mechanism of base pairing. Because each strand can be used to make the other strand, the strands are said to be complementary. DNA copies itself through the process of replication: The two strands of the double helix unzip, forming replication forks. New bases are a ...
Ethanol precipitation of DNA with salts
... interested in forming ion pairs between the polyanion (DNA) and the cation (Na+, Mg++, etc). In dilute aqueous solution, DNA and counterions like Na+ and Mg++ are more or less in the free ion form rather than the ion pair form (that is, each ion is surrounded by one or more layers of water molecules ...
... interested in forming ion pairs between the polyanion (DNA) and the cation (Na+, Mg++, etc). In dilute aqueous solution, DNA and counterions like Na+ and Mg++ are more or less in the free ion form rather than the ion pair form (that is, each ion is surrounded by one or more layers of water molecules ...
Inherited traits are traits that you get from your parents
... 20) DNA has the ability to make an exact copy of itself. Draw and explain how DNA Replicates. Why is this ability important for life to continue? The DNA molecule splits apart and each ½ strand of DNA is used as a template to make a new molecule. Each new DNA molecule is an exact copy of the origina ...
... 20) DNA has the ability to make an exact copy of itself. Draw and explain how DNA Replicates. Why is this ability important for life to continue? The DNA molecule splits apart and each ½ strand of DNA is used as a template to make a new molecule. Each new DNA molecule is an exact copy of the origina ...
Supramolecular Factories Inspired by Processive Enzymes
... unidirectionally along the track, initiating specific chemical reactions as it ...
... unidirectionally along the track, initiating specific chemical reactions as it ...
Ch 13 Genetic Engineering
... • Transforming Plant Cells – Using bacterium that normally infect plant cells and cause tumors – Taking away the cell wall some plant cells will take up DNA on their own – If successful recombinant DNA will be found in a chromosome of the cell ...
... • Transforming Plant Cells – Using bacterium that normally infect plant cells and cause tumors – Taking away the cell wall some plant cells will take up DNA on their own – If successful recombinant DNA will be found in a chromosome of the cell ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.