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AP Biology - HPHSAPBIO
AP Biology - HPHSAPBIO

... 23. Explain the general process of transcription, including the three major steps of initiation, elongation, and termination. 24. Explain how RNA is modified after transcription in eukaryotic cells. 25. Define and explain the role of ribozymes. 26. Describe the functional and evolutionary significan ...
DNA Structure and Function
DNA Structure and Function

Unit 10 powerpoint bio 134
Unit 10 powerpoint bio 134

... •  5.  The  enzyme  that  puts  new  bases  down   during  replicaMon  is  called:   •  6.  Errors  in  DNA  are  called  _______________.   ...
DNA Recombination - Home - KSU Faculty Member websites
DNA Recombination - Home - KSU Faculty Member websites

... Get your new recombinant plasmid back into the bacteria. This is easy because bacteria will take in DNA that’s floating around near them. We ...
MITOCHONDIAL GENETICS
MITOCHONDIAL GENETICS

... Error correction is a property of some, but not all, DNA polymerases. This process corrects mistakes in newly-synthesized DNA. When an incorrect base pair is recognized, DNA polymerase reverses its direction by one base pair of DNA. The 3'->5' exonuclease activity of the enzyme allows the incorrect ...
How do we know that DNA carries genetic information?
How do we know that DNA carries genetic information?

... Overview of the History of Molecular Biology  1929 – the chemical components of DNA  1940 – the relationship between genes and proteins  1944 – Genes are made up of DNA  1953 – the double helical structure of the DNA molecule (Watson and Crick)  1957 – the “Central Dogma” and the “sequence hyp ...
protein synthesis lab
protein synthesis lab

... To define different types of mutations. To understand the three types of point mutations; silent, missense, and nonsense. To understand how an addition or deletion of a nucleotide causes a frameshift mutation. To understand the four types of chromosomal mutations; deletion, duplication, inversion, t ...
DNA Structure and Replication
DNA Structure and Replication

Chapter 12 - SCA Moodle
Chapter 12 - SCA Moodle

... certain nitrogen bases and provide enough force to hold the two strands together • Hydrogen bonds could only form between certain base pairs adenine and thymine and guanine and cytosine • This principal is called Base pairing • This explains Chargaff’s Rule ...
DNA  1. Evidence for DNA as the genetic material.
DNA 1. Evidence for DNA as the genetic material.

... Each time the cell divides, one strand of DNA cannot be completely replicated so a single-stranded section remains. Enzymes remove these single-stranded sections. iii. As a result, the chromosomes get shorter each time the cell divides. iv. To prevent the loss of genetic information at the ends of t ...
What is a DNA?
What is a DNA?

... • Capable of self-replication and synthesis of RNA. • Contains instructions for our body cells to perform their specific functions. ...
From Hard Drives to Flash Drives to DNA Drives
From Hard Drives to Flash Drives to DNA Drives

... As our need for high-capacity information storage continues to increase, several researchers have begun to explore the possibility of using DNA for this purpose.4 The very fabric of life uses a binary code, but instead of the 1s and 0s computers use, the code in our DNA is composed of 4 letters: A, ...
Strawberry DNA Extraction
Strawberry DNA Extraction

... All living organisms contain DNA. Some fruits are especially suited for DNA extractions due to their multiple sets of chromosomes. Strawberries are octoploid, which means they have 8 copies of each chromosome (human body cells are diploid; they contain two copies of each chromosomes). DNA extraction ...
Student Activity PDF - TI Education
Student Activity PDF - TI Education

... How many separate strands of DNA are in the double helix? A. 0 B. 1 C. 2 D. 3 ...
Exam III 1710 F '01 Sample.doc
Exam III 1710 F '01 Sample.doc

... alter their phenotype by use or disuse of a character (stretch the neck, for example) and that such aquired characteristics could be passed on to the following generation. The scientist best known for this theory was: a. Lamarck. b. Mendel. c. Morgan. d. Darwin. e. Barr. ...
E1. Sticky ends, which are complementary in their DNA sequence
E1. Sticky ends, which are complementary in their DNA sequence

... E5. First, the chromosomal DNA that contains the source of the gene that you want to clone must be obtained from a cell (tissue) sample. A vector must also be obtained. The vector and chromosomal DNA are digested with a restriction enzyme. They are mixed together to allow the sticky ends of the DNA ...
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Slide 1

lec36_2013 - Andrew.cmu.edu
lec36_2013 - Andrew.cmu.edu

... - cut within, nuclease - cleave nucleic acid]. Used by bacteria to degrade invading viral DNA. Named after bacterial species the particular enzyme was isolated from, i.e. Eco = E. Coli. 1. Enzyme binds to specific recognition sequences with near absolute specificity and high affinity (KD = 10-10 M). ...
Chimpanzee DNA - AISG SP Moodle
Chimpanzee DNA - AISG SP Moodle

... Name ________________ Investigating Common Descent One of the most common misconceptions about human evolution is seen in the statement “humans came from apes.” This statement assumes that organisms evolve through a step-by-step progression from lower forms to higher forms of life and the direct tra ...
DNA * Deoxyribonucleic Acid
DNA * Deoxyribonucleic Acid

... damaged or changed which can alter the genetic message. – Many mutations happen spontaneously when DNA is replicating. – Some mutations can occur when DNA is exposed to mutagenic factors such as x-rays, UV radiation, and chemicals. ...
12Johnson
12Johnson

... • Because so much DNA is being replicated in the many cells of the body, there is a potential for errors to occur  DNA repair involves comparing the daughter strand to the parent DNA template to check for mistakes • the proofreading is not perfect because mutations are still possible, although rare ...
7.1 DNA Introduction
7.1 DNA Introduction

... Erwin Chargaff (1947): Chargaff rules: A = T, C = G Hershey & Chase (1952): confirmation that DNA is ...
HCS604.03 Exercise 1 Dr. Jones Spring 2005 Recombinant DNA
HCS604.03 Exercise 1 Dr. Jones Spring 2005 Recombinant DNA

... combination will result in bacterial colonies that are blue. The multiple cloning site in the plasmid is located within the α -peptide coding region of ß –galactosidase. When this is disrupted by insertion of foreign DNA, complementation does not occur and ß – galactosidase activity is produced. Bac ...
pUC18 DNA HAE III Digest (D6293) - Datasheet - Sigma
pUC18 DNA HAE III Digest (D6293) - Datasheet - Sigma

... 1 µg/ml ethidium bromide, 8 bands (80–587 bp) were clearly resolved and the pattern was consistent with the expected fragment sizes. Note: Ethidium bromide background can be reduced by destaining 30–45 minutes in 1× electrophoresis buffer. Precautions and Disclaimer This product is for R&D use only, ...
DNA Barcoding and the International Barcode of Life Initiative
DNA Barcoding and the International Barcode of Life Initiative

... 85K. Importantly, DNA barcoding enjoys proportionally higher and increasing research contributions from developing countries, compared to some older and larger technologyintensive disciplines, such as Genomics (Adamowicz and Steinke, in review). Growth of the barcode library. The DNA barcode library ...
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DNA repair



DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.
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