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DNA and RNA Structure
DNA and RNA Structure

... Nucleic acids are essential materials found in all living organisms. Their main function is to maintain and transmit the genetic code. This information is stored in the form of long polymer chains. Although the information they carry is one-dimensional, it is essential to understand the 3D structure ...
lecture 12, part 2, dna technology, 050509c
lecture 12, part 2, dna technology, 050509c

Exam 2
Exam 2

... and seeing which infection results in virus replication indicating genetic material was present. If the RNase treated sample from the virulent can transform the avirulent virus to virulent virus, that means RNA is not the genetic material,. If the DNase treated sample from the virulent can transform ...
1 kb
1 kb

... - rapid method to generate large amounts of specific segment of DNA (product usually < 10 kb in length) - need very small amount of template DNA …but can lead to contamination problems - need prior sequence info to design primers ...
Plasmid Isolation
Plasmid Isolation

Gel Electrophoresis – Virtual Lab
Gel Electrophoresis – Virtual Lab

... as they move. The smaller fragments of DNA are able to navigate faster than the larger ones, so they make it further down the gel than the larger molecules. The larger DNA fragments travel slower due to resistance and will be closer to the original side of the gel. This is how gel electrophoresis se ...
Assay for Methylation of genes
Assay for Methylation of genes

... From kindred’s with HNPCC, MSI is detected in as many as 85-95% of cases. The key characteristic of HNPCC is MSI caused by a defective DNA mismatch repair system. HNPCC accounts for about 3-5% of all colorectal cancer. For patients with suspected HNPCC, an attractive, cost-effective strategy is t ...
Genetic Profiling using Short Tandem Repeat Analysis
Genetic Profiling using Short Tandem Repeat Analysis

... several highly variable sites in the genome. Thus, its value lies in the fact that it is  based on genotype not phenotype.  A DNA profile, or genetic fingerprint, can be  obtained from saliva left on a stamp, cigarette butt, or even on the mouthpiece of  a telephone. Analysts can make a profile of t ...
DNA Structure and Function
DNA Structure and Function

... • DNA segments connected by ligase ...
DNA Extraction from Fruit - DNALC Lab Center
DNA Extraction from Fruit - DNALC Lab Center

... Have another student read step 5 from the procedure, while the rest of the class listens. Instruct students to draw a diagram of what will happen the precipitation with ethanol is completed in step 5. Make sure students understand that ethanol is a type of alcohol, similar to rubbing alcohol. Becaus ...
DNA TEST
DNA TEST

... 18. The DNA of a certain organism has cytosine as 22% of its bases. What percentage of the bases are thymine? a) 28% b) 78% c) 50% d) 22% 19. Semi conservative replication means that a) Sometimes DNA can replicate and sometimes it cannot, this accounts for aging b) Sometimes newly made DNA molecules ...
Bio 102 Practice Problems
Bio 102 Practice Problems

... Fill in the blanks: Several different enzymes are required in the complex process of DNA replication. The functions of some of these enzymes are described below; fill in each blank with the name of the appropriate enzyme. 1. _________________ is required to make phosphodiester bonds between two adj ...
DNA fingerprinting Genes and DNA
DNA fingerprinting Genes and DNA

... Lab #4 – Analysis of Results • p2 = the expected frequency of the (+/+) genotype in a population • 2pq = the expected frequency of the (+/-) genotype in a population • q2 = the expected frequency of the (-/-) genotype in a population ...
Wildlife Forensics Pre-Visit Lesson This pre
Wildlife Forensics Pre-Visit Lesson This pre

... Students should have a working knowledge of DNA. We expect students to be familiar enough with DNA to know that it organized into chromosomes found in the nucleus of eukaryotic cells. Whether the organism is a bacterium, fungus, plant, or animal there is DNA in the organism’s cells. Each cell conta ...
File
File

... were made using different fragments of DNA taken from O. sativa indica. The plants were then submerged for a period of 11 days. The heights of all the plants were measured at the beginning and at the end of the submergence period. ...
Microbiology 7/e
Microbiology 7/e

...  Cells must copy their chromosomes (DNA synthesis) before they divide so that each daughter cell will have a copy  A region of the chromosome remains uncopied (centromere) in order to hold the sister chromatids together – Keeps chromatids organized to help make sure each daughter cell gets exactly ...
13-2 Manipulating DNA
13-2 Manipulating DNA

... a photocopy machine stuck on "print," a technique known as polymerase chain reaction (PCR) allows biologists to do exactly that. The figure below shows how PCR works. The idea behind PCR is surprisingly simple. At one end of a piece of DNA a biologist wants to copy, he or she adds a short piece of D ...
Chapter 2 DNA to end Extended Response
Chapter 2 DNA to end Extended Response

... a. (genetic) variation in population; b. (variation is) due to mutation / sexual reproduction; c. valid example of variation in a specific population; d. more offspring are produced than can survive / populations over-populate; e. competition / struggle for resources/survival; f. example of competit ...
18 - cloudfront.net
18 - cloudfront.net

... a photocopy machine stuck on "print," a technique known as polymerase chain reaction (PCR) allows biologists to do exactly that. The figure below shows how PCR works. The idea behind PCR is surprisingly simple. At one end of a piece of DNA a biologist wants to copy, he or she adds a short piece of D ...
Chapter 4 - Version B
Chapter 4 - Version B

... 26. The percentage composition of a nucleic acid molecule found in bacterial cells is 32.3% adenine 30.7% thymine 19.1% cytosine 17.9% guanine The molecule is most likely to be a. double-stranded DNA. b. mitochondrial DNA. c. messenger RNA. ...
Chapter 4 - Version A
Chapter 4 - Version A

... 17. During DNA replication, one of the new strands of DNA is synthesized continuously, while the other is synthesized as a number of separate fragments of DNA that are subsequently linked by DNA ligase. This is because a. replication starts at many points on the chromosome b. RNA primers only anneal ...
Worksheet 1 (isolation)
Worksheet 1 (isolation)

... Besides the coding information (exons), DNA contains a lot of non-coding information (introns). During RNA processing these non-coding parts are removed. Before the synthesis of a protein starts, the corresponding RNA molecule is formed by RNA transcription. One strand of the DNA double helix is use ...
CRISPR-Cas9 in gene therapy: much control on breaking
CRISPR-Cas9 in gene therapy: much control on breaking

ppt
ppt

... Besides the coding information (exons), DNA contains a lot of non-coding information (introns). During RNA processing these non-coding parts are removed. Before the synthesis of a protein starts, the corresponding RNA molecule is formed by RNA transcription. One strand of the DNA double helix is use ...
Document
Document

... DNA replication machinery then uses each 3’-OH on target DNA as a primer and copies the transposon ...
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DNA repair



DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.
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