Ways to detect unique sequences within mammalian DNA
... EX: humans have 3 billion base pairs with 1 million restriction fragments formed from a single restriction enzyme digest - TOO difficult to isolate a single band on a gel from this large number of fragments To characterize a specific gene use blot hybridization - see Figure 1 - WE DID THIS!! ...
... EX: humans have 3 billion base pairs with 1 million restriction fragments formed from a single restriction enzyme digest - TOO difficult to isolate a single band on a gel from this large number of fragments To characterize a specific gene use blot hybridization - see Figure 1 - WE DID THIS!! ...
You Light Up My Life
... Explain How It Duplicates • DNA is two nucleotide strands held together by hydrogen bonds • Hydrogen bonds between two strands are easily broken ...
... Explain How It Duplicates • DNA is two nucleotide strands held together by hydrogen bonds • Hydrogen bonds between two strands are easily broken ...
Part 1 – History, DNA Structure, DNA Replication
... Read the text and answer the following questions. 1. What have people wondered since the beginning of human history? _________________________________ 2. Who discovered that individual traits are passed on from one generation to the next? In what year?________________________________________________ ...
... Read the text and answer the following questions. 1. What have people wondered since the beginning of human history? _________________________________ 2. Who discovered that individual traits are passed on from one generation to the next? In what year?________________________________________________ ...
DNA Quiz - BiologySemester58
... selection on the quiz paper. ____ 21. In addition to the DNA in the nucleus, DNA is also found in the chloroplasts and mitochondria of eukaryotic cells. ____ 22. Maurice Wilkins used X-ray crystallography on DNA fibres, which suggested that DNA was helical in structure. ____ 23. Chargaff's rules sta ...
... selection on the quiz paper. ____ 21. In addition to the DNA in the nucleus, DNA is also found in the chloroplasts and mitochondria of eukaryotic cells. ____ 22. Maurice Wilkins used X-ray crystallography on DNA fibres, which suggested that DNA was helical in structure. ____ 23. Chargaff's rules sta ...
Spatial ordering and abnormal optical activity of DNA liquid
... because there are disordered water molecules between the ds DNA molecules. The ds DNA molecules possess some disorder around their positions; they can slide and bent with respect of each other, as well as they can rotate around their long axis. It corresponds to the liquid character of their packing ...
... because there are disordered water molecules between the ds DNA molecules. The ds DNA molecules possess some disorder around their positions; they can slide and bent with respect of each other, as well as they can rotate around their long axis. It corresponds to the liquid character of their packing ...
DNA: Structure, Function, and History
... Rosalind Franklin X-ray crystallographer that produced image Watson and Crick used to determine helical structure with two strands ...
... Rosalind Franklin X-ray crystallographer that produced image Watson and Crick used to determine helical structure with two strands ...
Applied molecular technique
... degrades the peptidoglycan layer of the cell wall. A successive treatment with detergent dissolves the lipids of the cell membrane. Chelating agents, such as EDTA (ethylene diamine tetraacetate), are also used, especially with gram-negative bacteria, to remove the metal ions that bind components of ...
... degrades the peptidoglycan layer of the cell wall. A successive treatment with detergent dissolves the lipids of the cell membrane. Chelating agents, such as EDTA (ethylene diamine tetraacetate), are also used, especially with gram-negative bacteria, to remove the metal ions that bind components of ...
Ch. 16 The Molecular Basis of Life
... can have errors in the replication of DNA 1 in 100,000 basepairs DNA polymerase proofreads the nucleotide with the template if an error is found, DNA polymerase removes the nucleotide and then continues synthesis if error is missed can get mismatch repair enzymes fix the incorrectly pair ...
... can have errors in the replication of DNA 1 in 100,000 basepairs DNA polymerase proofreads the nucleotide with the template if an error is found, DNA polymerase removes the nucleotide and then continues synthesis if error is missed can get mismatch repair enzymes fix the incorrectly pair ...
DNA to Protein - Louisiana Believes
... relieve the double helix thermodynamic strain. This allows a point of rotation as the two halves of the DNA are separated by weakening the hydrogen bonds. The double strands are then pulled apart, creating a Y formation called the replication fork. At the replication fork, the sequence of bases is u ...
... relieve the double helix thermodynamic strain. This allows a point of rotation as the two halves of the DNA are separated by weakening the hydrogen bonds. The double strands are then pulled apart, creating a Y formation called the replication fork. At the replication fork, the sequence of bases is u ...
Which is not correct?
... These are examples of a A. Frame shift mutation B. Point mutation When talking about point mutations, it is important to remember which bases are purines (A/G) and which are pyrimidines (C/T). When a point mutation causes a purine to convert to another purine (for example, C to T), this is known as ...
... These are examples of a A. Frame shift mutation B. Point mutation When talking about point mutations, it is important to remember which bases are purines (A/G) and which are pyrimidines (C/T). When a point mutation causes a purine to convert to another purine (for example, C to T), this is known as ...
Plasmid w/ kanamycin resistance (pKAN)
... • Mix plasmids with restriction enzymes – BamH1 and Hind III – Restriction enzymes cut the plasmids at precise locations ...
... • Mix plasmids with restriction enzymes – BamH1 and Hind III – Restriction enzymes cut the plasmids at precise locations ...
7. Recombinant DNA Vectors
... artificial chromosome vectors (BACs, PACs, YACs)--cloning chromosomal regions b. Conventional E. coli plasmid cloning vectors typically have: origin of replication that functions in bacteria antibiotic resistance gene(s) selectable marker gene (often lacZ, encoding beta-galactosidase) polylinker (al ...
... artificial chromosome vectors (BACs, PACs, YACs)--cloning chromosomal regions b. Conventional E. coli plasmid cloning vectors typically have: origin of replication that functions in bacteria antibiotic resistance gene(s) selectable marker gene (often lacZ, encoding beta-galactosidase) polylinker (al ...
DNA Replication Simulation WKST
... Now you are ready to start adding new DNA nucleotides using the old strands. Before DNA replication can start, a primer must be added so DNA polymerase can bind. A primer is a short stretch of RNA that initiates DNA replication by allowing polymerase to bind and add nucleotides to the end of it. Pri ...
... Now you are ready to start adding new DNA nucleotides using the old strands. Before DNA replication can start, a primer must be added so DNA polymerase can bind. A primer is a short stretch of RNA that initiates DNA replication by allowing polymerase to bind and add nucleotides to the end of it. Pri ...
Heidi Sleister
... – Strawberries are missing from the school garden. – A search led to 5 people with strawberries. – Do any of these strawberries match the strawberries from the garden? ...
... – Strawberries are missing from the school garden. – A search led to 5 people with strawberries. – Do any of these strawberries match the strawberries from the garden? ...
DNA/RNA
... 5. Find the arrows on each model nucleotide. Find the 3’ end marked on the sugar. The arrow points toward the 3’ end. Arrows are important when building DNA strands. 6. Build a small DNA molecule with just 4 nucleotides: A, T, G, and C. Your DNA should look like a ladder, with 2 nucleotides on each ...
... 5. Find the arrows on each model nucleotide. Find the 3’ end marked on the sugar. The arrow points toward the 3’ end. Arrows are important when building DNA strands. 6. Build a small DNA molecule with just 4 nucleotides: A, T, G, and C. Your DNA should look like a ladder, with 2 nucleotides on each ...
New Measurements of DNA Twist Elasticity
... The idea of studying the response of DNA to mechanical stress is as old as the discovery of the double helix structure itself. While many elements of DNA function require detailed understanding of specific chemical bonds (for example the binding of small ligands), still others are quite nonspecific ...
... The idea of studying the response of DNA to mechanical stress is as old as the discovery of the double helix structure itself. While many elements of DNA function require detailed understanding of specific chemical bonds (for example the binding of small ligands), still others are quite nonspecific ...
13.2 – Manipulating DNA
... Because significant amounts of a sample of DNA are necessary for molecular and genetic analyses, studies of isolated pieces of DNA are nearly impossible without PCR amplification. Often heralded as one of the most important scientific advances in molecular biology, PCR revolutionized the study o ...
... Because significant amounts of a sample of DNA are necessary for molecular and genetic analyses, studies of isolated pieces of DNA are nearly impossible without PCR amplification. Often heralded as one of the most important scientific advances in molecular biology, PCR revolutionized the study o ...
The wrong file for Lecture 8 was posted on the website. I`ve sent the
... There are a number of different moderately repetitive sequences. Minisatellites are variable number tandem repeats (VNTPs). The sequences are variable in length (10-100 bp), but within a repeat sequence, the individual sequences will be the same. VNTPs create regions of 1000-5000 bp in length ...
... There are a number of different moderately repetitive sequences. Minisatellites are variable number tandem repeats (VNTPs). The sequences are variable in length (10-100 bp), but within a repeat sequence, the individual sequences will be the same. VNTPs create regions of 1000-5000 bp in length ...
DNA Structure DNA Structure
... replication but some errors still occur replication, occur. These mistakes or mutations are a mixed blessing. They provide the genetic variation that is essential for evolution but, unfortunately, most are harmful. ...
... replication but some errors still occur replication, occur. These mistakes or mutations are a mixed blessing. They provide the genetic variation that is essential for evolution but, unfortunately, most are harmful. ...
9.3 DNA Fingerprinting
... – The probability that two people share identical numbers of repeats in several locations is ...
... – The probability that two people share identical numbers of repeats in several locations is ...
MUTATIONS - Valhalla High School
... • May produce a new trait or may result in a protein that does not work correctly • In some rare cases, it may have a positive effect • Can be passed on to offspring ...
... • May produce a new trait or may result in a protein that does not work correctly • In some rare cases, it may have a positive effect • Can be passed on to offspring ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.