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DNAfor NathanLec - Sonoma State University
DNAfor NathanLec - Sonoma State University

... a. Form a bond between - 3’ OH group of deoxyribose on last nucleotide - 5’ phosphate of the correct dNTP b. Add nucleotide and release diphosphate c. Move to next nucleotide on template B. Initiation of DNA replication 1. Origins of replication a. Specific sequence of nucleotides b. recognized by p ...
DNA
DNA

... tRNA has anticodon (3 bases that code for a specific amino acid) tRNA matches its anticodon with the mRNA codon and drops off its amino acid to bond with the next amino acid Continues until a “stop codon” is reached ...
The History of DNA
The History of DNA

... • Mice were injected with either R(rough) strain of Streptococcus pneumoniae. The mice live and their immune system kills R bacteria. No live bacteria • Mice injected with the S (smooth) strain of Streptococcus pneumoniae. The mice die. The dead mice have live S bacteria. • Mice injected with heat-k ...
Pretest Ch 12: DNA - Aurora City School
Pretest Ch 12: DNA - Aurora City School

... Prokaryotic DNA replication is different than eukaryotic. How…? 17. What shape does prokaryotic DNA have? ...
DNA Replication - Bi-YOLO-gy
DNA Replication - Bi-YOLO-gy

... 7. How many mRNA strands are produced during transcription? ____________________ 8. A group of 3 mRNA bases is called a _______________________. 9. If a DNA strand reads GCATAGATTACA, what is the complimentary strand made during transcription? 10. Where does mRNA travel to at the end of transcriptio ...
Oct. 5
Oct. 5

... Who figured out (& got the Nobel Prize) the full 3D structure of DNA? ...
DNA Nucleotides Nitrogenous Base Complimentary Base Pairs
DNA Nucleotides Nitrogenous Base Complimentary Base Pairs

... complimentary nitrogenous base pairs (AT or CG). ...
DNA: The material of heredity
DNA: The material of heredity

... DNA replication occurs with a high degree of accuracy, but some errors occur. Even one error can have a serious effect ...
Structure of DNA - Plain Local Schools
Structure of DNA - Plain Local Schools

... 4. If six bases on one strand of DNA are AGTCGG what are the six bases on lthe complementary section of the other strand of DNA? ...
A O R P T Y S
A O R P T Y S

... • Nitrogen bases are read in units of 3 called codons • Each codon represents 1 amino acid ...
DNA Structure and Replication
DNA Structure and Replication

... 1. How many base pairs do all our 46 chromosomes in every one of our cells contain? 2. What do you notice about the two strands of DNA? http://www.fed.cuhk.edu.hk/~johnson/teaching/genetics/animations/dna_replication.htm 1. Explain role of DNA Helicase. 2. DNA polymerase: http://www.hhmi.org/biointe ...
DNA
DNA

...  Chemicals that act like scissors to cut DNA molecules at specific locations ...
DNA- Replication - Seabreeze High School
DNA- Replication - Seabreeze High School

... Replication has “proof-readers” to help reduce errors ...
Biology 20
Biology 20

... 10 nucleotide pairs in each helix turn DNA strands are antiparallel (p. 191; Fig. 10.5B) Why would a cell undergo DNA replication? ...
DNA Structure and Replication
DNA Structure and Replication

... http://nobelprize.org/educational_games/medicine/dna_double_helix/dnahelix.html 1. What is the ‘spiral staircase’ really called? 2. The “rungs” are made of? Click “Organism 1” to start copying both sides of its DNA strand. The better/faster you are, the less mutations! 3. How many mutations did you ...
three possibile models for replication
three possibile models for replication

... 9. Two of the nitrogen bases (A and G) have a double-ring structure. These bases are called purines. 10. Two of the nitrogen bases (T and C) have a single-ring structure. These bases are called pyrimidines. 11. Nitrogen bases from opposite strands bond together using hydrogen bonds across the double ...
DNA polymerase
DNA polymerase

... •DNA replication is the process of copying a DNA molecule. Replication is semiconservative, with each strand of the original double helix (parental molecule) serving as a template (mold or model) for a new strand in a daughter molecule. This process consists of: •Unwinding (initiation): old strands ...
File
File

... a. uses energy from the hydrolysis of 3. DNA gyrase- reduces tortional strain – prevents 4. ssb – single strand binding protein – increases stability in an environment 5. DNA pol I – removes primers and replaces them with 6. DNA ligase – joins fragments “link em with ligase” ...
Working with Data The Meselson–Stahl Experiment
Working with Data The Meselson–Stahl Experiment

... gradient to separate the labeled molecules based on density. After the first round of replication, all of the DNA was of intermediate density, proving the conservative model false. After the second round of replication, half of the DNA was intermediate and half was light only, proving the dispersive ...
Biochemistry 6/e
Biochemistry 6/e

...  Defective DNA mismatch repair (1/200)  hMSH2 (MutS) and hMLH1 (MutL) p53: mutated in more than half of all tumors  Sensing double strand breaks  Activating repair systems or apoptosis ...
DNA - Mrs. Smith`s Biology Class
DNA - Mrs. Smith`s Biology Class

... pairs differ in various organisms? ...
25/100 bp Mixed DNA Ladder DNA Molecular Weight Markers
25/100 bp Mixed DNA Ladder DNA Molecular Weight Markers

... ● Description : 25/100 bp Mixed DNA Ladder is specially designed for determining the size of double strand DNA from 25 to 2,000 base pairs. The DNA Ladder consists of 17 double strand DNA fragments ranging in size from 25 to 200 bp in 25 bp increments, and additional fragments of 300, 400, 500, 600, ...
SBI4U-Molecular Genetics Molecular Genetics Unit Test –Multiple
SBI4U-Molecular Genetics Molecular Genetics Unit Test –Multiple

... 6) If a free phosphate is found at the 5’ end of a DNA strand, what is found at the other end of the same strand? a) A hydroxyl group on the 5’ carbon of a deoxyribose sugar. b) A hydroxyl group on the 3’ carbon of a deoxyribose sugar. c) A phosphate group on the 5’ carbon of a deoxyribose sugar. d) ...
12-2 Notes
12-2 Notes

... (about 1.6 mm)  This DNA must be folded to 1/1000th of its length just to fit inside the bacteria ...
The Fourth Macromolecule!!!
The Fourth Macromolecule!!!

... copies) ...
< 1 ... 34 35 36 37 38 39 40 41 42 ... 55 >

Eukaryotic DNA replication



Eukaryotic DNA replication is a conserved mechanism that restricts DNA replication to only once per cell cycle. Eukaryotic DNA replication of chromosomal DNA is central for the duplication of a cell and is necessary for the maintenance of the eukaryotic genome.DNA replication is the action of DNA polymerases synthesizing a DNA strand complementary to the original template strand. To synthesize DNA, the double-stranded DNA is unwound by DNA helicases ahead of polymerases, forming a replication fork containing two single-stranded templates. Replication processes permit the copying of a single DNA double helix into two DNA helices, which are divided into the daughter cells at mitosis. The major enzymatic functions carried out at the replication fork are well conserved from prokaryotes to eukaryotes, but the replication machinery in eukaryotic DNA replication is a much larger complex, coordinating many proteins at the site of replication, forming the replisome.The replisome is responsible for copying the entirety of genomic DNA in each proliferative cell. This process allows for the high-fidelity passage of hereditary/genetic information from parental cell to daughter cell and is thus essential to all organisms. Much of the cell cycle is built around ensuring that DNA replication occurs without errors.In G1 phase of the cell cycle, many of the DNA replication regulatory processes are initiated. In eukaryotes, the vast majority of DNA synthesis occurs during S phase of the cell cycle, and the entire genome must be unwound and duplicated to form two daughter copies. During G2, any damaged DNA or replication errors are corrected. Finally, one copy of the genomes is segregated to each daughter cell at mitosis or M phase. These daughter copies each contain one strand from the parental duplex DNA and one nascent antiparallel strand.This mechanism is conserved from prokaryotes to eukaryotes and is known as semiconservative DNA replication. The process of semiconservative replication for the site of DNA replication is a fork-like DNA structure, the replication fork, where the DNA helix is open, or unwound, exposing unpaired DNA nucleotides for recognition and base pairing for the incorporationof free nucleotides into double-stranded DNA.
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