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DNA Replication
DNA Replication

... Chargaff showed the amounts of the four bases on DNA ( A,T,C,G)  In a body or somatic cell: A = 30.3% T = 30.3% G = 19.5% C = 19.9% ...
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The structure and mechanism of DNA gyrase from divergent

... The enzyme alters the level of supercoiling through the concerted passage of a segment of DNA through a double-stranded DNA gap made by the A subunits. This process utilises the free energy from the hydrolysis of ATP by the B subunits. The sequences of several type I1 topoisomerases from phage, bact ...
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... N formed two bands: one DNA band was at the ...
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44 DNA and Its Role in Heredity
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... _______ 10.) Who determined that the DNA molecule was a helix (using Xray diffraction)? a.) Chargaff b.) Watson and Crick c.) Rosalind Franklin d.) Mendel Complete the next two questions on this handout only! 11.) “Match” the DNA base pairs with their proper “partners” (in other words, I will give y ...
DNA Function in Heredity Chapter 11
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chapter 16 – the molecular basis of inheritance
chapter 16 – the molecular basis of inheritance

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The DNA strand that is replicated smoothly and continuously is

... 1. is a site where one DNA strand serves as a template, but the other strand is not replicated. 2. is created by the action of the enzyme RNA polymerase. 3. is only seen in prokaryotic chromosomes. 4. is a Y-shaped structure where both DNA strands are replicated simultaneously. ...
DNA replication
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... The genetic code is composed of triplets: one triplet encode one amino acid The genetic code is redundant: many amino acids are encoded by more than one triplets The genetic code is „comma-free”: the triplets are not isolated units The genetic code is universal: every living being is descended from ...
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DNA replication - Cloudfront.net
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Eukaryotic DNA replication



Eukaryotic DNA replication is a conserved mechanism that restricts DNA replication to only once per cell cycle. Eukaryotic DNA replication of chromosomal DNA is central for the duplication of a cell and is necessary for the maintenance of the eukaryotic genome.DNA replication is the action of DNA polymerases synthesizing a DNA strand complementary to the original template strand. To synthesize DNA, the double-stranded DNA is unwound by DNA helicases ahead of polymerases, forming a replication fork containing two single-stranded templates. Replication processes permit the copying of a single DNA double helix into two DNA helices, which are divided into the daughter cells at mitosis. The major enzymatic functions carried out at the replication fork are well conserved from prokaryotes to eukaryotes, but the replication machinery in eukaryotic DNA replication is a much larger complex, coordinating many proteins at the site of replication, forming the replisome.The replisome is responsible for copying the entirety of genomic DNA in each proliferative cell. This process allows for the high-fidelity passage of hereditary/genetic information from parental cell to daughter cell and is thus essential to all organisms. Much of the cell cycle is built around ensuring that DNA replication occurs without errors.In G1 phase of the cell cycle, many of the DNA replication regulatory processes are initiated. In eukaryotes, the vast majority of DNA synthesis occurs during S phase of the cell cycle, and the entire genome must be unwound and duplicated to form two daughter copies. During G2, any damaged DNA or replication errors are corrected. Finally, one copy of the genomes is segregated to each daughter cell at mitosis or M phase. These daughter copies each contain one strand from the parental duplex DNA and one nascent antiparallel strand.This mechanism is conserved from prokaryotes to eukaryotes and is known as semiconservative DNA replication. The process of semiconservative replication for the site of DNA replication is a fork-like DNA structure, the replication fork, where the DNA helix is open, or unwound, exposing unpaired DNA nucleotides for recognition and base pairing for the incorporationof free nucleotides into double-stranded DNA.
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