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DNA and RNA - CK
DNA and RNA - CK

... in which DNA is copied. It occurs during the synthesis (S) phase of the eukaryotic cell cycle. DNA replication begins when an enzyme breaks the bonds between complementary bases in DNA (see Figure 1.4). This exposes the bases inside the molecule so they can be “read” by another enzyme and used to bu ...
genomic library
genomic library

... • Restriction enzymes cut DNA into specific fragments • Restriction enzymes recognize specific base sequences in double-stranded DNA and cleave both strands of the duplex at specific places • Characteristics of restriction enzymes: 1. Cut DNA sequence-specifically 2. Bacterial enzymes; hundreds are ...
DNA Replication
DNA Replication

... History of DNA • Early scientists thought protein was the cell’s hereditary material because it was more complex than DNA • Proteins were composed of 20 different amino acids in long polypeptide chains ...
March 13
March 13

... introns are self-splicing (type II): no spliceosomes or other enzymes! 2) mRNA editing:many cp mRNAs differ from the gene encoding them •an ACG is modified post-transcriptionally to a functional AUG start codon in several tobacco mRNAs; many other post-transcriptional changes have also been identifi ...
DNA Replication Reading - Lesley Anderson`s Digital Portfolio
DNA Replication Reading - Lesley Anderson`s Digital Portfolio

... in the body. A few cells from a drop of blood or from saliva on a cigarette butt are all detectives need to produce a DNA “fingerprint” of a criminal suspect. Apply How does replication ensure that cells have complete sets of DNA? MAIN IDEA ...
DNA Profiling
DNA Profiling

... crime or to the scene of a crime. • If some biological tissue (saliva on a cigarette butt, semen stain or a hair) is found at a crime scene, its DNA profile is compared with one taken from a suspect. • If patterns match, suspect is associated with the crime scene. Note: See Figure 16.18 pg. 155 – co ...
DNA - morescience
DNA - morescience

... working with Drosophila (fruit flies) genes are on chromosomes but is it the protein or the DNA of the chromosomes that are the genes?  through 1940 proteins were thought to be genetic material… Why? What’s so impressive about proteins?! ...
RayBio Genomic DNA Magnetic Beads Kit
RayBio Genomic DNA Magnetic Beads Kit

... supernatant using a pipette, without disturbing the beads that have collected at the magnet. 10. Remove the tube from the magnetic separator and add 500 µL Wash Solution 2 (prepared with ethanol, see section 6.C). Vortex briefly to resuspend the beads and return the tube to the magnetic separator fo ...
Name_______________ Pre-Assessment
Name_______________ Pre-Assessment

... Below is a description of steps to DNA replication. However, the steps of DNA replication are out of order. Match each step with the part of the DNA replication it represents. Record the corresponding letter for that step next to its corresponding part in DNA replication. Also, mark whether you are ...
Host-Microbiome Research Network Germ
Host-Microbiome Research Network Germ

... GTG AAT CAT CGA ATC TTT GAA ...
APDC Unit IX CC DNA Bio
APDC Unit IX CC DNA Bio

... 1. Describe how a plasmid can be genetically modified to include a piece of foreign DNA that alters the phenotype of bacterial cells transformed with the modified plasmid. 2. How can a genetically modified organism provide a benefit for humans and at the same time pose a threat to a population or ec ...
DNA Notes Name_____________________________ assign
DNA Notes Name_____________________________ assign

... GA Biology Standards: SB2. Students will analyze how biological traits are passed on to successive generations. a. Distinguish between DNA & RNA. b. Explain the role of DNA in storing & transmitting cellular information. d. Describe the relationships between changes in DNA and potential appearance ...
Chapter 16
Chapter 16

... hydroxyl at another end. 7. The two DNA strands are antiparallel, that is, their sugar phosphate backbones run in opposite directions. ...
Page 1 AP Biology TEST #5 - Chapters 11-14, 16
Page 1 AP Biology TEST #5 - Chapters 11-14, 16

... 59. DNA fingerprinting works because A) genes containing the same alleles make it simple to compare different individuals. B) PCR allows amplification of proteins from single cells. C) there are multiple alleles for some DNA sequences, making it possible to obtain unique patterns for each individual ...
I Current Comments@ EUGENE GA/?FlELi2
I Current Comments@ EUGENE GA/?FlELi2

... high impact work, rather than formally summarize the technical gist of the research. Some of these one-page commentaries cettainly deserve to be expanded into articles or books. But the brevity of Ciration Classics” commentaries is one of its main vittues. While some authors find it challenging to ...
DNA modelling - Teacher instructions - Lesson element
DNA modelling - Teacher instructions - Lesson element

... Using the instruction sheet learners should decide which coloured pipe cleaner represents each DNA base and also keep in mind its correlating pair. Any cuts that need to be made should then be done. Once cut to shape the pairs of colours should be matched and the ends twisted together. Learners shou ...
Comp 5a Packet
Comp 5a Packet

... 7. Did Griffith's experiment prove DNA was the genetic material? 8. What 2 main things make up chromosomes? 9. What did Hershey and Chase use in their experiments to prove DNA was the cell's genetic material? 10. What scientists showed the amount of the 4 nitrogen bases present in DNA? 11. Name the ...
DNA the Crown Jewels 2012
DNA the Crown Jewels 2012

... 5. mtDNA analysis is more sensitive than nuclear DNA profiling but it is also more time consuming and expensive. 6. Another problem is that all maternally related people have the same mtDNA. 7. In cases involving a missing or long deceased, an mtDNA reference sample could be obtained from any matern ...
Gregor Mendel—Overview - National Science Teachers Association
Gregor Mendel—Overview - National Science Teachers Association

... nine. When she was eleven, she attended a public day school in London, St. Paul’s ...
Experiment 2 Plasmid DNA Isolation, Restriction Digestion and Gel
Experiment 2 Plasmid DNA Isolation, Restriction Digestion and Gel

... The alkaline lysis preparation is the most commonly used method for isolating small amounts of plasmid DNA, often called minipreps. This method uses SDS as a weak detergent to denature the cells in the presence of NaOH, which acts to hydrolyze the cell wall and other cellular molecules. The high pH ...
DNA Replication Practice Test Answer Section
DNA Replication Practice Test Answer Section

... ____ 18. The addition of nucleotides to form a complementary strand of DNA a. is catalyzed by DNA polymerase. b. is accomplished only in the presence of tRNA. c. prevents separation of complementary strands of RNA. d. is the responsibility of the complementary DNA mutagens. ____ 19. Which of the fol ...
No Slide Title
No Slide Title

... How does sugar pucker change distance between phosphates? Know diff erence between syn and anti - when do you see syn? Know all the hydrogen bond donors and acceptors in the Watson-Crick base pairs How do donors and acceptors change when the enol form of the bases are found vs. the keto form? What b ...
lecture notes
lecture notes

... phenotype of Dam- mutants (mutator)- same spectrum as others phenotype if overproduce dam methylase- also mutator, not enough time to distinguish strands OH=> Dam- is hypersensitive to 2-aminopurine. Idea (pg 476 of 3rd ed) is that 2AP is normally incorporated but causes bulge that is recognized by ...
dna - Nutley Public Schools
dna - Nutley Public Schools

DNA EVIDENCE: Officials admit error, dismiss case
DNA EVIDENCE: Officials admit error, dismiss case

... includes a conviction for aggravated stalking. Although he had served out his sentence for this crime, such a serious criminal record gave the Immigration and Naturalization Service the right to take him into custody while they determined whether he should be allowed to remain in the United States. ...
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DNA profiling



DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.
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