Lack of biological significance in the `linguistic features` of
... 2 of the original paper of Mantegna et al. (2). To be concrete, we present here (in Fig. 1a) the corresponding graphs, for 6-tuples, of the human sequence HSRETBLAS (1.5% coding) and the Escherichia coli sequence ECUW89 (82.1% coding) DNAs, as also studied by Mantegna et al. (2). (The mentioned acro ...
... 2 of the original paper of Mantegna et al. (2). To be concrete, we present here (in Fig. 1a) the corresponding graphs, for 6-tuples, of the human sequence HSRETBLAS (1.5% coding) and the Escherichia coli sequence ECUW89 (82.1% coding) DNAs, as also studied by Mantegna et al. (2). (The mentioned acro ...
Modified PDF
... thermal inactivation (Lindenbaum et al. 1986) and binding between the two proteins has been observed employing immobilized DBP (B. van Breukelen, unpublished). However, most other common assays to demonstrate such an interaction, such as pull-down or immune precipitation, have been unsuccessful sugg ...
... thermal inactivation (Lindenbaum et al. 1986) and binding between the two proteins has been observed employing immobilized DBP (B. van Breukelen, unpublished). However, most other common assays to demonstrate such an interaction, such as pull-down or immune precipitation, have been unsuccessful sugg ...
An Unusual Sugar Conformation in the Structure of an RNA/DNA
... of the minus strand at the start of reverse transcription, the viral RNA recruits a host tRNA forming an RNA/RNA duplex. However once (2 )-strand DNA synthesis begins, a chimeric hybrid, an Okazaki fragment, is formed at the junction of the growing DNA chain with the tRNA/RNA. This junction has been ...
... of the minus strand at the start of reverse transcription, the viral RNA recruits a host tRNA forming an RNA/RNA duplex. However once (2 )-strand DNA synthesis begins, a chimeric hybrid, an Okazaki fragment, is formed at the junction of the growing DNA chain with the tRNA/RNA. This junction has been ...
The DnaE polymerase from Deinococcus radiodurans features
... interacts with the core α subunit. The β-clamp is a homodimer, and confers to the holoenzyme high processivity by tethering the polymerase to the DNA substrate [20, 21]. In addition, the progression of a replication fork is assisted by the association of DNA Pol III with DNA helicase and primase [22 ...
... interacts with the core α subunit. The β-clamp is a homodimer, and confers to the holoenzyme high processivity by tethering the polymerase to the DNA substrate [20, 21]. In addition, the progression of a replication fork is assisted by the association of DNA Pol III with DNA helicase and primase [22 ...
Cleavage of a model DNA replication fork by a Type I restriction
... proAB+ lacIqZDM15]} was a gift from Dr Akio Nomoto (University of Tokyo). JM109 (DE3) was constructed using a DE3 Lysogenization Kit (Novagen) following the manufacturer’s protocol. A plasmid for expression of the EcoR124I R subunit, pACR124, and one for its M and S subunit, pJS4M, were kindly prov ...
... proAB+ lacIqZDM15]} was a gift from Dr Akio Nomoto (University of Tokyo). JM109 (DE3) was constructed using a DE3 Lysogenization Kit (Novagen) following the manufacturer’s protocol. A plasmid for expression of the EcoR124I R subunit, pACR124, and one for its M and S subunit, pJS4M, were kindly prov ...
Chapter 13 Mutation, DNA Repair, and Recombination
... Mismatch Repair in E. coli Mismatching or mispairing of G and T (DNA polymerase/exonuclease proofreading activity) The A in GATC sequences is methylated subsequent to DNA replication. In newly replicated DNA, the parental strand is methylated, but the new strand is not. This difference allows ...
... Mismatch Repair in E. coli Mismatching or mispairing of G and T (DNA polymerase/exonuclease proofreading activity) The A in GATC sequences is methylated subsequent to DNA replication. In newly replicated DNA, the parental strand is methylated, but the new strand is not. This difference allows ...
Title Heterochromatin Blocks Constituting the Entire
... small apes).2,13 Similar to Hominidae, this family includes species such as white-handed and hoolock gibbons that do not have large subtelomeric heterochromatin blocks.14 This sporadic distribution of heterochromatin blocks among species suggests that such blocks undergo rapid increase and decrease ...
... small apes).2,13 Similar to Hominidae, this family includes species such as white-handed and hoolock gibbons that do not have large subtelomeric heterochromatin blocks.14 This sporadic distribution of heterochromatin blocks among species suggests that such blocks undergo rapid increase and decrease ...
Can Jury Trial Innovations Improve Juror Understanding of DNA
... is found in the nucleus of each cell and is unique to each individual (except in cases of identical twins). Nuclear DNA is a powerful identifier and has been used for forensic purposes for decades. Mitochondrial DNA (mtDNA)—which is found in the mitochondria of a cell, outside of the nucleus and sep ...
... is found in the nucleus of each cell and is unique to each individual (except in cases of identical twins). Nuclear DNA is a powerful identifier and has been used for forensic purposes for decades. Mitochondrial DNA (mtDNA)—which is found in the mitochondria of a cell, outside of the nucleus and sep ...
Chapter 12 Recombinant DNA Technology Key Concepts
... gene from the genome. Although it is relatively easy to isolate DNA from living tissue, DNA in a test tube looks like a glob of mucus. How could it be possible to isolate a single gene from this tangled mass of DNA threads? Recombinant DNA technology provides the techniques for doing just that, and ...
... gene from the genome. Although it is relatively easy to isolate DNA from living tissue, DNA in a test tube looks like a glob of mucus. How could it be possible to isolate a single gene from this tangled mass of DNA threads? Recombinant DNA technology provides the techniques for doing just that, and ...
Generalized Transduction by Phage P22 in Salmonella typhimurium. II. Mechanisms of Integration of Transducing DNA.
... the fate of the DNA of these particles in recipient bacteria. Our studies on the formation of P22 transducing particles, presented in the preceding paper, show that P22 transducing particles, like Pl transducing particles (Ikeda & Tomizawa, 1965), contain predominantly bacterial DNA which was synthe ...
... the fate of the DNA of these particles in recipient bacteria. Our studies on the formation of P22 transducing particles, presented in the preceding paper, show that P22 transducing particles, like Pl transducing particles (Ikeda & Tomizawa, 1965), contain predominantly bacterial DNA which was synthe ...
Identification and removal of colanic acid from plasmid DNA
... co-purify with DNA by anion-exchange chromatography. The commercially available laboratory scale purification methods, including Qiagen, use anionexchange chromatography for at least one major step in the purification process. Finally, on the basis of their similar buoyant densities, many polysaccha ...
... co-purify with DNA by anion-exchange chromatography. The commercially available laboratory scale purification methods, including Qiagen, use anionexchange chromatography for at least one major step in the purification process. Finally, on the basis of their similar buoyant densities, many polysaccha ...
Resolution of the type material of the Asian elephant, Elephas
... Sequences of 4258 bp of mtDNA (from MT-ND5 to control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were de ...
... Sequences of 4258 bp of mtDNA (from MT-ND5 to control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were de ...
Genetic Markers for Sex Identification in Forensic DNA Analysis
... individual based on DNA evidence in instances such as identification of victims of mass disaster, missing persons investigations, and sexual assault cases. Analysis of Y-specific target sequences on the Y chromosome is a largely effective method for determining the chromosomal sex of an individual a ...
... individual based on DNA evidence in instances such as identification of victims of mass disaster, missing persons investigations, and sexual assault cases. Analysis of Y-specific target sequences on the Y chromosome is a largely effective method for determining the chromosomal sex of an individual a ...
Resolution of the type material of the Asian elephant, Elephas
... Sequences of 4258 bp of mtDNA (from MT-ND5 to control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were de ...
... Sequences of 4258 bp of mtDNA (from MT-ND5 to control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were de ...
DNA damage in round spermatids of mice with a targeted disruption
... The mixture of tissue and medium was drawn up into a Pasteur pipette and transferred into a polystyrene Falcon conical centrifuge test tube and vortexed. If the specimen contained spermatozoa, a percoll gradient (45–90%; 400 g for 30 min) was used at this point to remove larger cells before injectio ...
... The mixture of tissue and medium was drawn up into a Pasteur pipette and transferred into a polystyrene Falcon conical centrifuge test tube and vortexed. If the specimen contained spermatozoa, a percoll gradient (45–90%; 400 g for 30 min) was used at this point to remove larger cells before injectio ...
biomolecules (introduction, structure and functions)
... factors, enzymes, splicing factors). Many of these proteins have a significant effect on DNA or RNA conformation. Interactions with proteins may be general or sequence specific and may involve subtle or overt changes in structure. The restriction enzymes EcoRI and EcoRV, for e.g., both introduce a p ...
... factors, enzymes, splicing factors). Many of these proteins have a significant effect on DNA or RNA conformation. Interactions with proteins may be general or sequence specific and may involve subtle or overt changes in structure. The restriction enzymes EcoRI and EcoRV, for e.g., both introduce a p ...
Jurisdiction, Privacy, and Ownership: DNA Technology and Field
... A mass fatality is an event resulting in more deaths than the local available resources can process.4 A mass fatality event can be manmade (armed conflict), natural disaster (tsunami), or accident that may be due to manmade or natural causes (plane crash). Mass fatalities occur under chaotic and unc ...
... A mass fatality is an event resulting in more deaths than the local available resources can process.4 A mass fatality event can be manmade (armed conflict), natural disaster (tsunami), or accident that may be due to manmade or natural causes (plane crash). Mass fatalities occur under chaotic and unc ...
Title A Fluorescently Labeled, Hyperbranched Polymer
... duplex, Scheme 1C) changes the structure of the polymer due to the strong ionic interaction between the two molecules, which leads to exposure of the rhodamine fluorophores in the process. The second step involves adding the complementary sequence to the solution containing the duplex. This forms a ...
... duplex, Scheme 1C) changes the structure of the polymer due to the strong ionic interaction between the two molecules, which leads to exposure of the rhodamine fluorophores in the process. The second step involves adding the complementary sequence to the solution containing the duplex. This forms a ...
Essential knowledge 3.A.1 - local.brookings.k12.sd.us
... Evidence of student learning is a demonstrated understanding of each of the following: 1. Both have three components — sugar, phosphate and a nitrogenous base — which form nucleotide units that are connected by covalent bonds to form a linear molecule with 3' and 5' ends, with the nitrogenous bases ...
... Evidence of student learning is a demonstrated understanding of each of the following: 1. Both have three components — sugar, phosphate and a nitrogenous base — which form nucleotide units that are connected by covalent bonds to form a linear molecule with 3' and 5' ends, with the nitrogenous bases ...
NJP17
... as the ‘recognition energy’, was first calculated in [14]. The theory of homology recognition was then developed with a higher level of sophistication in a series of subsequent works (for review see [10, 15], and for a recent study [16] with citations to later articles contained therein). The recogni ...
... as the ‘recognition energy’, was first calculated in [14]. The theory of homology recognition was then developed with a higher level of sophistication in a series of subsequent works (for review see [10, 15], and for a recent study [16] with citations to later articles contained therein). The recogni ...
number of fifty human tumours
... of chromosome counts were made, it is evident that except for Tumour No. 29 there is quite good agreement between basic DNA content and chromosome number. For Tumour No. 29, however, the observed chromosome number is approximately twice that expected from the DNA data. This discrepancy is possibly d ...
... of chromosome counts were made, it is evident that except for Tumour No. 29 there is quite good agreement between basic DNA content and chromosome number. For Tumour No. 29, however, the observed chromosome number is approximately twice that expected from the DNA data. This discrepancy is possibly d ...
Validation of picogram- and femtogram-input DNA libraries
... Genomic DNA extracted from 40 bacterial and 14 archaeal taxa for which reference genomes are available (54 isolate genomes), were combined to create a mock community (Table S1). Purified genomic DNAs from 49 of the 54 mock community members were obtained from collaborators. These DNAs were assessed ...
... Genomic DNA extracted from 40 bacterial and 14 archaeal taxa for which reference genomes are available (54 isolate genomes), were combined to create a mock community (Table S1). Purified genomic DNAs from 49 of the 54 mock community members were obtained from collaborators. These DNAs were assessed ...
The Structure and Function of Nucleic Acids
... A study of the structure and function of nucleic acids is needed to be able to understand how information controlling the characteristics of an organism is stored in the form of genes in a cell and how these genes are transmitted to future generations of offspring. The rapid developments in the area ...
... A study of the structure and function of nucleic acids is needed to be able to understand how information controlling the characteristics of an organism is stored in the form of genes in a cell and how these genes are transmitted to future generations of offspring. The rapid developments in the area ...
The Preservation and Persistence of Human DNA in Soil during
... of DNA of a particular organism does not necessarily apply to all other organisms, as many of the processes leading to the release of extracellular DNA are not fully ...
... of DNA of a particular organism does not necessarily apply to all other organisms, as many of the processes leading to the release of extracellular DNA are not fully ...
DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.