Real-time Quantification of HER2/neu Gene Amplification by
... The techniques used to evaluate the HER2/neu gene status have included gene-based assays such as Southern and slot blotting, in-situ hybridization (fluorescent and nonfluorescent) and PCR methods [7]. Each of the techniques mentioned has its advantages and disadvantages. In order to perform a fast p ...
... The techniques used to evaluate the HER2/neu gene status have included gene-based assays such as Southern and slot blotting, in-situ hybridization (fluorescent and nonfluorescent) and PCR methods [7]. Each of the techniques mentioned has its advantages and disadvantages. In order to perform a fast p ...
Impact of Sample Type and DNA Isolation Procedure on
... derived from the different DNA isolation methods using DESeq2 analyses. In pairwise ...
... derived from the different DNA isolation methods using DESeq2 analyses. In pairwise ...
PDF - The Journal of General Physiology
... Intact Phage.--The intact phage was mixed with the Ilford G-5 emulsion on three different dates corresponding to 0, 40, and 50 per cent of the incorporated p3~ decayed. A total of nine different emulsions was counted; the average star size extended from 7.4 to 15.4 rays per star. The average of thes ...
... Intact Phage.--The intact phage was mixed with the Ilford G-5 emulsion on three different dates corresponding to 0, 40, and 50 per cent of the incorporated p3~ decayed. A total of nine different emulsions was counted; the average star size extended from 7.4 to 15.4 rays per star. The average of thes ...
Detection of two novel porcine herpesviruses with high similarity to
... Evidence for the existence of porcine gammaherpesviruses was obtained by PCR and sequence analysis. Initially, samples of peripheral blood mononuclear cells (PBMC), spleens, lungs, kidneys and livers of pigs from Germany and Spain were tested with a PCR assay which targets conserved regions of the h ...
... Evidence for the existence of porcine gammaherpesviruses was obtained by PCR and sequence analysis. Initially, samples of peripheral blood mononuclear cells (PBMC), spleens, lungs, kidneys and livers of pigs from Germany and Spain were tested with a PCR assay which targets conserved regions of the h ...
Lecture 19 POWERPOINT here
... Cloning and growing • One can use the techniques of cell biology to manufacture artificial and real products, be they genes, proteins, or organisms • If you want to insert some DNA into another molecule then the best place to start is to use the same restriction enzyme to cut both - so they have th ...
... Cloning and growing • One can use the techniques of cell biology to manufacture artificial and real products, be they genes, proteins, or organisms • If you want to insert some DNA into another molecule then the best place to start is to use the same restriction enzyme to cut both - so they have th ...
1. The figure below represents a water molecule. H O H Water
... Explain how these three properties help organisms survive in the pond. In your answer you should make clear the links between the behaviour of the water molecules and the survival of the organisms. ...
... Explain how these three properties help organisms survive in the pond. In your answer you should make clear the links between the behaviour of the water molecules and the survival of the organisms. ...
Organization and Synthesis of DNA
... Cross-shaped structures arise from palindromic structures, including interrupted palindromes like this example These are less stable than regular duplexes but they are common, and they do create recognition sites for DNA-binding proteins, including restriction enzymes ...
... Cross-shaped structures arise from palindromic structures, including interrupted palindromes like this example These are less stable than regular duplexes but they are common, and they do create recognition sites for DNA-binding proteins, including restriction enzymes ...
Multifractal analysis of DNA sequences using a novel chaos
... used to probe the range of correlation of the sequences [4,5]. Linguistic features were claimed to have been found in noncoding DNA sequences [6], a point that has provoked controversy [7–10]. Still others have emphasized the fractality hidden in some or other representations of the sequences [11–14 ...
... used to probe the range of correlation of the sequences [4,5]. Linguistic features were claimed to have been found in noncoding DNA sequences [6], a point that has provoked controversy [7–10]. Still others have emphasized the fractality hidden in some or other representations of the sequences [11–14 ...
Magnusiomyces capitatus (de Hoog et al.) de Hoog et Smith
... 1. Fungal genomic DNA is provided in a dried form. Store at +2°C to 8°C upon receipt. Store at 20°C if stored for more than 6 months). Note: Do not store in freezers with a defrost cycle. This will expose the product to increased temperatures. 2. Concentration by PicoGreen® measurement was foun ...
... 1. Fungal genomic DNA is provided in a dried form. Store at +2°C to 8°C upon receipt. Store at 20°C if stored for more than 6 months). Note: Do not store in freezers with a defrost cycle. This will expose the product to increased temperatures. 2. Concentration by PicoGreen® measurement was foun ...
A-10484A SNPs. Mutations and DNA Sequence
... using a panel of 10 SNPs. PCR products were obtained for each individual SNP as separate reactions. Primers were designed so that none of the products exceeded 1000 bps in size. During design they were also masked for repeats using the human repeat database. PCR products were verified on a agarose g ...
... using a panel of 10 SNPs. PCR products were obtained for each individual SNP as separate reactions. Primers were designed so that none of the products exceeded 1000 bps in size. During design they were also masked for repeats using the human repeat database. PCR products were verified on a agarose g ...
The Mechanism of Insertion of a Segment of
... transformants obtained, suggesting that this restriction site lies relatively close to the position of the pheA mutation. Digestion with SalGI reduced the number of Nic+ transformants about tenfold. These results have been used to assign tentative positions to the pheA and nic genes on the restricti ...
... transformants obtained, suggesting that this restriction site lies relatively close to the position of the pheA mutation. Digestion with SalGI reduced the number of Nic+ transformants about tenfold. These results have been used to assign tentative positions to the pheA and nic genes on the restricti ...
Forensics Test Key
... the suspects (16). The suspect may have worn gloves (17). The fingerprints could have been from people who came to see the show (18). The glass most closely matches common glass (19). None of the suspects had any pieces of common glass on them (20). The shopping list may not have had anything to do ...
... the suspects (16). The suspect may have worn gloves (17). The fingerprints could have been from people who came to see the show (18). The glass most closely matches common glass (19). None of the suspects had any pieces of common glass on them (20). The shopping list may not have had anything to do ...
DNA Pre-ConceptionStu - the Biology Scholars Program Wiki
... C. Protein, a molecule of nitrate and one of four nitrogen containing bases D. Sugar, a molecule of phosphate and one of four amino acids E. Sugar, a molecule of phosphate and one of four nitrogen containing bases 5. Which one of the following substances is found in DNA but not in RNA? A. Uracil B. ...
... C. Protein, a molecule of nitrate and one of four nitrogen containing bases D. Sugar, a molecule of phosphate and one of four amino acids E. Sugar, a molecule of phosphate and one of four nitrogen containing bases 5. Which one of the following substances is found in DNA but not in RNA? A. Uracil B. ...
the mass spectrometry-based method EpiTYPER
... 22. This file contains the sequences and masses of each so-called “CpG unit”, that is a fragment containing one or multiple CpG sites. The CpG sites are numbered from the 5′ end of the genomic sequence. After each unit there might be a warning message. If the fragment has another (non-) CpG containi ...
... 22. This file contains the sequences and masses of each so-called “CpG unit”, that is a fragment containing one or multiple CpG sites. The CpG sites are numbered from the 5′ end of the genomic sequence. After each unit there might be a warning message. If the fragment has another (non-) CpG containi ...
The Role of DNA Structure and Dynamics in the
... and indirect readout mechanisms. The two symmetric half-sites ACCG·CGGT are highly conserved in the genomes and are hydrogen bound with E2. Although E2 does not contact the N4 spacer, the affinities are modulated by the base composition of this DNA part. Nevertheless, the origin of either the global ...
... and indirect readout mechanisms. The two symmetric half-sites ACCG·CGGT are highly conserved in the genomes and are hydrogen bound with E2. Although E2 does not contact the N4 spacer, the affinities are modulated by the base composition of this DNA part. Nevertheless, the origin of either the global ...
A Human Centromere Protein, CENP-B, Has a DNA Binding Domain
... Steuer et al., 1990). Several monoclonal antibodies that recognize the centromeric region of human chromosomes have been isolated using scaffold proteins as antigens (Cooke et al., 1987; Compton et al., 1991); one of the antigens, a 250300-kD protein, was found to be localized to centromere only at ...
... Steuer et al., 1990). Several monoclonal antibodies that recognize the centromeric region of human chromosomes have been isolated using scaffold proteins as antigens (Cooke et al., 1987; Compton et al., 1991); one of the antigens, a 250300-kD protein, was found to be localized to centromere only at ...
Bio11U_Ch 6_approvedcopyedit_100817
... 4. What paths of investigation should research scientists take with respect to DNA manipulation? What are the ethical considerations associated with genetic technology? ...
... 4. What paths of investigation should research scientists take with respect to DNA manipulation? What are the ethical considerations associated with genetic technology? ...
USB® Thermo Sequenase Cycle Sequencing Kit
... and ddNTPs are balanced so that the majority of chains will be terminated within the desired length of sequence. When these fragments are separated on a suitable gel matrix, sequence information can be obtained from the order of the bands. Cycle sequencing(5-12) uses repeated cycles of thermal denat ...
... and ddNTPs are balanced so that the majority of chains will be terminated within the desired length of sequence. When these fragments are separated on a suitable gel matrix, sequence information can be obtained from the order of the bands. Cycle sequencing(5-12) uses repeated cycles of thermal denat ...
DNA without Warrant - UR Scholarship Repository
... Vitruvian Man of privacy and personhood within the squared circle of constitutionality.'0 Arrestee gene capture is the latest piece in the identification-investigation puzzle that already includes faces and bodies (mugshots, showups and lineups), eyes (color, retina and iris scans), fingertips (fing ...
... Vitruvian Man of privacy and personhood within the squared circle of constitutionality.'0 Arrestee gene capture is the latest piece in the identification-investigation puzzle that already includes faces and bodies (mugshots, showups and lineups), eyes (color, retina and iris scans), fingertips (fing ...
24.5 Nucleic Acids
... DNA typing uses the variation in the DNA of individuals as a basis for creating DNA profiles to identify a person from samples of his or her hair, skin cells, or body fluid. • Because DNA sequences, like fingerprints, are unique for each individual, DNA typing has also been called DNA fingerprinting ...
... DNA typing uses the variation in the DNA of individuals as a basis for creating DNA profiles to identify a person from samples of his or her hair, skin cells, or body fluid. • Because DNA sequences, like fingerprints, are unique for each individual, DNA typing has also been called DNA fingerprinting ...
DNA Polyacrylamide Gel Electrophoresis
... V/cm) to prevent denaturation of small fragments of DNA by heating. Other electrophoresis buffers such as 1x TAE can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much buffering capacity as TBE. For electrophoresis runs greater than 8 ...
... V/cm) to prevent denaturation of small fragments of DNA by heating. Other electrophoresis buffers such as 1x TAE can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much buffering capacity as TBE. For electrophoresis runs greater than 8 ...
PcrA Helicase Tightly Couples ATP Hydrolysis to Unwinding Double
... rapid mixing of RepD with Junction 3, in which the fluorescein is attached at the 50 -end of the ICRII arm rather than the end of the duplex. The traces (Figure 4a) show a rapid rise in anisotropy followed by a slow decrease to a level below that of the initial DNA. This would be expected from rapid ...
... rapid mixing of RepD with Junction 3, in which the fluorescein is attached at the 50 -end of the ICRII arm rather than the end of the duplex. The traces (Figure 4a) show a rapid rise in anisotropy followed by a slow decrease to a level below that of the initial DNA. This would be expected from rapid ...
G-quadruplex and G-rich sequence stimulate Pif1p
... and gene expression regulatory regions such as promoters (4). The existence of G4 in living cells has been confirmed using an engineered antibody that can recognize G4 structure with high affinity and specificity (5,6). Initial computational analyses have revealed that there are >375 000 G4 motifs i ...
... and gene expression regulatory regions such as promoters (4). The existence of G4 in living cells has been confirmed using an engineered antibody that can recognize G4 structure with high affinity and specificity (5,6). Initial computational analyses have revealed that there are >375 000 G4 motifs i ...
PDF version - EpiGeneSys
... saturation, and thus chromatin fibre compaction. However, this method consumes a large amount of material, and thus is often not practical.(comment 7) In some circumstances, a thorough analysis of protein content may be necessary ? see (Huynh et al, 2005). This may be the case when working with unus ...
... saturation, and thus chromatin fibre compaction. However, this method consumes a large amount of material, and thus is often not practical.(comment 7) In some circumstances, a thorough analysis of protein content may be necessary ? see (Huynh et al, 2005). This may be the case when working with unus ...
molecular biology
... four different sugar ring conformations are designated as C-2' endo or C-2' exo, C-3' endo or C-3' exo. In the C-2' endo structure, the 2' carbon lies above the furanose ring on the same side as the base and the 5'-carbon. C-3' exo structure is quite similar. In C-3' endo structure, it is the 3' car ...
... four different sugar ring conformations are designated as C-2' endo or C-2' exo, C-3' endo or C-3' exo. In the C-2' endo structure, the 2' carbon lies above the furanose ring on the same side as the base and the 5'-carbon. C-3' exo structure is quite similar. In C-3' endo structure, it is the 3' car ...
DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.