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Powerpoint show
Powerpoint show

... Vertical bars represent mRNA identified as cDNA clones ...
Abundance of an mRNA is the average number of molecules per cell
Abundance of an mRNA is the average number of molecules per cell

... Bidirectional replication is accomplished when two replication forks move away from the same origin in different directions. Bivalent is the structure containing all four chromatids (two representing each homologue) at the start of meiosis. Blastoderm is a stage of insect embryogenesis in which a la ...
The Nucleic Acid World
The Nucleic Acid World

... It is amazing to realize that the full diversity of life on this planet—from the simplest bacterium to the largest mammal—is captured in a linear code inside all living cells. In almost exactly the way the vivid detail of a musical symphony or a movie can be digitally recorded in a binary code, so t ...
Name: Date: Period: ______ Gel Electrophoresis Lab Ms. Ottolini
Name: Date: Period: ______ Gel Electrophoresis Lab Ms. Ottolini

... Revisions for your Online Lab Portfolio: If you choose to use this lab for your portfolio, please note the following revisions… 1. You will not create a purpose or hypothesis section for this lab. 2. You will not include a methods summary chart. Instead, your “methods” section will include the foll ...
Cytogenetic and AZF microdeletions on the Y chromosome of
Cytogenetic and AZF microdeletions on the Y chromosome of

... by these primer sets have been associated with male infertility [12, 16, 19-27]. The primers have been combined into five Multiplex Master Mix sets for use in multiplex PCR. This makes it possible to determine the presence or absence of all 20 STS by performing five concurrent PCR amplifications (Fi ...
Chapter 11 Nucleic Acids and Protein Synthesis
Chapter 11 Nucleic Acids and Protein Synthesis

... • Write the sequence for the mRNA that could be synthesized using the following DNA base sequence as a template: ...
Upwelling, Downwelling, and El Nino
Upwelling, Downwelling, and El Nino

... Primer is a short RNA segment that is complementary to DNA segment & that is necessary to begin DNA replication Primers are polymerized by an enzyme called primase Portion of parental DNA serves as template for primer w a base sequence that is about 10 nucleotides long in eukaryotes Primer formation ...
Extensive Nuclear DNA Sequence Diversity Among Chimpanzees
Extensive Nuclear DNA Sequence Diversity Among Chimpanzees

... Although data on nucleotide sequence variation in the human nuclear genome have begun to accumulate, little is known about genomic diversity in chimpanzees (Pan troglodytes) and bonobos (Pan paniscus). A 10,154 – base pair sequence on the chimpanzee X chromosome is reported, representing all major s ...
Absence of unidentified CAG repeat expansion in
Absence of unidentified CAG repeat expansion in

... repeat expansions. However, the performance of this method does not allow detection of small expansions (such as those found in SCA6) as the threshold is about 40 repeats. ERDA-1 and CTG18.1 loci represent polymorphic and unstable sequences which may explain RED scores of 40 repeats or more. Therefo ...
Lab resulting
Lab resulting

... parse to AMRS.These is however specific to v/load and dna pcr. d) In ampath care lab,v/loads and dna pcr which are processed using EID system,we receive a .csv file upon dispatch of results.The .csv file is parsed through MIRTH for hl7 generation which is later parsed to AMRS. e) In khuyangu,Teso,Bu ...
06Molecular Basis of Inhertance
06Molecular Basis of Inhertance

... or RNA). A nucleotide has three components – a nitrogenous base, a pentose sugar (ribose in case of RNA, and deoxyribose for DNA), and a phosphate group. There are two types of nitrogenous bases – Purines (Adenine and Guanine), and Pyrimidines (Cytosine, Uracil and Thymine). Cytosine is common for b ...
New Mutations in the KVLQT1 Potassium Channel That Cause Long
New Mutations in the KVLQT1 Potassium Channel That Cause Long

... episodic ventricular tachyarrhythmias, specifically torsade de pointes and ventricular fibrillation.9 –11 Inherited LQTS can result from at least five different genes. Four genes were mapped to chromosome 11p15.5 (LQT1),12,13 7q35–36 (LQT2),14 3p21–24 (LQT3),14 and 4q25–27 (LQT4).15 Several other fa ...
Analysis by pulsed-field gel electrophoresis mutations in the
Analysis by pulsed-field gel electrophoresis mutations in the

... The following DNA probes were used: (i) the 1.3-kb BamHI fragment of pUC4K12 that encodes the nptll gene; (ii) the 2.9-kb BamHI/HindIII fragment of pG4.214that encodes TBPl from N . meningitidis; and (iii) the 2.1-kb EcoRI/HindIII fragment from pSD. 1615 that encodes TBP2 from N. meningitidis. DNA f ...
File
File

... The resulting mutations may or may not affect an organism. Some mutations that affect individual organisms can also affect a species or even an entire ecosystem. ...
Molecular Basis of Inhertance
Molecular Basis of Inhertance

... or RNA). A nucleotide has three components – a nitrogenous base, a pentose sugar (ribose in case of RNA, and deoxyribose for DNA), and a phosphate group. There are two types of nitrogenous bases – Purines (Adenine and Guanine), and Pyrimidines (Cytosine, Uracil and Thymine). Cytosine is common for b ...
Student`s guide -
Student`s guide -

... the samples provided, there is a difference between the DNA sequences of the alleles D and d. In d, there is a base-pair sequence that can be ‘recognised’ and cut by the restriction enzyme BamHI. In contrast, D has no restriction site and therefore it cannot be cut by the enzyme. Individuals who are ...
Targeted Investigation of the Neandertal Genome by Array
Targeted Investigation of the Neandertal Genome by Array

... regions, ruling out experiments such as the retrieval of exomes, large chromosomal regions, or validation of sites of interest identified in the low-coverage shotgun genome data. Because microarrays can carry hundreds of thousands of probes, we investigated the use of massively parallel hybridizatio ...
Chapter 13-14 Review
Chapter 13-14 Review

... Know the double helix model of DNA, and who proposed it Know the structure of a nucleotide, and of DNA, chemical bonding Know the three types of RNA and their functions Know what a gene is, and what it codes for, and how Beadle and Tatum’ experiment lad to this hypothesis Know what Chargaff’s rules ...
The Role of Mismatch Repair in Bacterial Evolution
The Role of Mismatch Repair in Bacterial Evolution

... The indirect selective benefit of mutators depends on opportunities for adaptation (28,30,31). The fraction of mutations that improve adaptation depends on the evolutionary history of the population in the new environment. If the population is already well adapted, then most if not all mutations wil ...
Hyper-eccentric structural genes in the mitochondrial genome of the
Hyper-eccentric structural genes in the mitochondrial genome of the

... (fig. 2A). The boundary position between the modules could be elucidated clearly, except in a few exceptional cases. For example, as the boundary between modules 7 and 8 overlapped by one T residue, no precise boundary was recognizable (fig. 2B). Five sites for U-insertion type of RNA editing were p ...
letters Structural basis for the diversity of DNA recognition by bZIP
letters Structural basis for the diversity of DNA recognition by bZIP

... nature sequence are supported by mutagenesis experiments that have shown increased binding of GCN4 to TTAGTAA when the corresponding bZIP residues (Ala and Cys/Ser) are substituted with Gln and Phe, respectively8. In addition, a V285Q mutant in CCAAT/enhancer binding protein (C/EBP) (Gln 90 in Pap1) ...
SAM Teachers Guide Nucleic Acids and Proteins - RI
SAM Teachers Guide Nucleic Acids and Proteins - RI

... hydrogen bonding is optimal when the shapes of the two molecules allow them to line up closely together.  Possible Discussion Question: • What is a hydrogen bond? Why is it important in biology? Emphasize that a hydrogen bond is just a polar attraction between molecules that occurs very frequently i ...
TG_REV_NAP_r6 - RI
TG_REV_NAP_r6 - RI

... hydrogen bonding is optimal when the shapes of the two molecules allow them to line up closely together. Possible Discussion Question:  What is a hydrogen bond? Why is it important in biology? Emphasize that a hydrogen bond is just a polar attraction between molecules that occurs very frequently in ...
12–1 DNA
12–1 DNA

... Griffith called this process transformation because one strain of bacteria (the harmless strain) had changed permanently into another (the diseasecausing strain). Griffith hypothesized that a factor must contain information that could change harmless bacteria into disease-causing ones. ...
Studies on Chlamydomonas Chloroplast Transformation: Foreign
Studies on Chlamydomonas Chloroplast Transformation: Foreign

... Taken together, the availability of chloroplast DNA mutants, the ability to correct stably deletions in the C. reinhardtii chloroplast genome with introduced homologous DNA, and the high degree of DNA sequence homology to the chloroplast genomes of higher plants suggest that this organism may be wel ...
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Microsatellite



A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.
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