Extensive protein and DNA backbone sampling
... fragment moves, in which a continuous stretch of base-paired DNA is replaced by a base paired region of equal length taken from a DNA duplex of known structure (Figure 2A); interface fragment moves, in which the orientation of the protein relative to the DNA is updated based on a template interface ...
... fragment moves, in which a continuous stretch of base-paired DNA is replaced by a base paired region of equal length taken from a DNA duplex of known structure (Figure 2A); interface fragment moves, in which the orientation of the protein relative to the DNA is updated based on a template interface ...
somatic hypermutation of the 5' noncoding region of the Frequent MARTINOrrI*t,
... from chromosomal rearrangements and linkage to immunoglobulin genes. These alterations identify a mechanism of genetic instability in malignant B cells and may have been selected during lymphomagenesis for their role in altering BCL6 expression. ...
... from chromosomal rearrangements and linkage to immunoglobulin genes. These alterations identify a mechanism of genetic instability in malignant B cells and may have been selected during lymphomagenesis for their role in altering BCL6 expression. ...
Dear Jennifer
... Bucks High School South, and Mr. Ippolito, Port Chester High School, for sharing helpful activities which provided us with many useful ideas. ...
... Bucks High School South, and Mr. Ippolito, Port Chester High School, for sharing helpful activities which provided us with many useful ideas. ...
Purification of Agrobacterium rhizogenes protein (GALLS) required
... Understanding expression of Agrobacterium rhizogenes proteins (GALLS) required for gene transfer to plants. ...
... Understanding expression of Agrobacterium rhizogenes proteins (GALLS) required for gene transfer to plants. ...
Chromosomal breakpoint positions suggest a direct role for radiation
... (Santoro et al., 1993). However, malignant phenotype was obtained by cotransfection with H-RAS or K-RAS oncogenes. An alternative to the direct pathway is that radiation does not act directly to produce the RET/PTC3 fusion, but instead may cause persistent genomic instability, which eventually produ ...
... (Santoro et al., 1993). However, malignant phenotype was obtained by cotransfection with H-RAS or K-RAS oncogenes. An alternative to the direct pathway is that radiation does not act directly to produce the RET/PTC3 fusion, but instead may cause persistent genomic instability, which eventually produ ...
An accessible database for mouse and human whole transcriptome
... We carried out an automatic primer design procedure to target all possible transcripts and all ‘possible’ exon–exon and intron– exon junctions in the human and mouse transcriptomes. Not all consecutive exon–intron–exon triplets generate ‘possible’ primer pairs (i.e. satisfy the constraints). The res ...
... We carried out an automatic primer design procedure to target all possible transcripts and all ‘possible’ exon–exon and intron– exon junctions in the human and mouse transcriptomes. Not all consecutive exon–intron–exon triplets generate ‘possible’ primer pairs (i.e. satisfy the constraints). The res ...
Rapid and accurate cancer somatic mutation
... in tumor tissue (i.e., high assay sensitivity). This tool must account ...
... in tumor tissue (i.e., high assay sensitivity). This tool must account ...
fourth quarter atlas analysis
... • Add A product peak fragment size and corresponding allelic ladder Genes in same size, was named digital ...
... • Add A product peak fragment size and corresponding allelic ladder Genes in same size, was named digital ...
A natural chimeric yeast containing genetic material from three species
... The Saccharomyces sp. ClDl isolate (CBS 8614) and several other Saccharomyces sensu stricto yeasts were analysed for their mitochondrial and nuclear genes. The data show that Saccharomyces sp. CID1, found so far only in one location in Europe, is a natural hybrid between three different Saccharomyce ...
... The Saccharomyces sp. ClDl isolate (CBS 8614) and several other Saccharomyces sensu stricto yeasts were analysed for their mitochondrial and nuclear genes. The data show that Saccharomyces sp. CID1, found so far only in one location in Europe, is a natural hybrid between three different Saccharomyce ...
Preventing transcriptional gene silencing by active DNA demethylation
... specific ribonuclease III, Dicer [16]. In several systems, these small interfering RNAs (siRNAs) have been shown to interact with other proteins such as Argonaute to form an RNA-induced silencing complex (RISC) and target homologous mRNAs for degradation [17,18]. Several well-known epigenetic phenome ...
... specific ribonuclease III, Dicer [16]. In several systems, these small interfering RNAs (siRNAs) have been shown to interact with other proteins such as Argonaute to form an RNA-induced silencing complex (RISC) and target homologous mRNAs for degradation [17,18]. Several well-known epigenetic phenome ...
Modified PDF
... hindsight this was not surprising, since DBP is a very abundant protein with at least 10 million copies per infected cell. Moreover, its strong interaction with ssDNA makes it easy to be detected. The DNA binding capacity and the genetically determined need for stoichiometric amounts immediately sug ...
... hindsight this was not surprising, since DBP is a very abundant protein with at least 10 million copies per infected cell. Moreover, its strong interaction with ssDNA makes it easy to be detected. The DNA binding capacity and the genetically determined need for stoichiometric amounts immediately sug ...
Supplementary Methods Tables
... TCGA data at the DCC is organized by platform, data type and data level. Each platform may produce several types of data. For example, SNP-based platforms yield three data types: SNP, Copy Number Results, and Loss of Heterozygosity (LOH). The TCGA concept of data level segregates raw data from deriv ...
... TCGA data at the DCC is organized by platform, data type and data level. Each platform may produce several types of data. For example, SNP-based platforms yield three data types: SNP, Copy Number Results, and Loss of Heterozygosity (LOH). The TCGA concept of data level segregates raw data from deriv ...
Male-Biased Mutation Rate and Divergence in Autosomal, Z
... adjustment was required to improve the alignment of repetitive sequences. Pairwise distances were estimated by use of the baseml program in PAML version 3.11 (Yang 1997), with the Tamura-Nei (Tamura and Nei 1993) model of sequence evolution. Distances were estimated on the assumption that all sites ...
... adjustment was required to improve the alignment of repetitive sequences. Pairwise distances were estimated by use of the baseml program in PAML version 3.11 (Yang 1997), with the Tamura-Nei (Tamura and Nei 1993) model of sequence evolution. Distances were estimated on the assumption that all sites ...
From Gene to Protein*Transcription and Translation
... There are multiple different types of tRNA. Each type of tRNA molecule has three nucleotides that form an anti-codon. The three nucleotides in the tRNA anti-codon are complementary to the three nucleotides in the mRNA codon for a specific type of amino acid. For each type of tRNA, a specific enzyme ...
... There are multiple different types of tRNA. Each type of tRNA molecule has three nucleotides that form an anti-codon. The three nucleotides in the tRNA anti-codon are complementary to the three nucleotides in the mRNA codon for a specific type of amino acid. For each type of tRNA, a specific enzyme ...
Nucleic acid hybridisation and polymerase chain reaction
... conventional pathogen detection procedures, while possessing the advantage that they are often able to produce results in a single day, thus providing the opportunity of taking more effective control measures. The presence of infectious agents in fluid or tissue samples can be revealed by microscopi ...
... conventional pathogen detection procedures, while possessing the advantage that they are often able to produce results in a single day, thus providing the opportunity of taking more effective control measures. The presence of infectious agents in fluid or tissue samples can be revealed by microscopi ...
Friedreich ataxia: The clinical picture
... required. Cognitive functions generally are well preserved, although subtle abnormalities, particularly of executive functions, have been detected [5]. Because it involves substantial physical disability, FRDA may have a substantial impact on academic, professional, and personal development. The neu ...
... required. Cognitive functions generally are well preserved, although subtle abnormalities, particularly of executive functions, have been detected [5]. Because it involves substantial physical disability, FRDA may have a substantial impact on academic, professional, and personal development. The neu ...
Mutations of the ret protooncogene in German multiple
... Germline mutations of the ret protooncogene have been identified as the underlying cause of the MEN 2 and FMTC syndromes (2-6). ret is a member of the family of receptor tyrosine kinases and is expressed in tissues derived from neural crest. Mutations at one of five codons for cysteine in the ret ex ...
... Germline mutations of the ret protooncogene have been identified as the underlying cause of the MEN 2 and FMTC syndromes (2-6). ret is a member of the family of receptor tyrosine kinases and is expressed in tissues derived from neural crest. Mutations at one of five codons for cysteine in the ret ex ...
GENECLEAN® Kit
... When working with radio-labeled DNA, add 1 µl of Label Block to each 10 µl of GLASSMILK® and incubate for 5 minutes at room temperature before adding to the DNA/NaI solution in the next step. 5. Add GLASSMILK® to the DNA/NaI solution as calculated in Step 3. Vortex gently or stir with a pipet tip to ...
... When working with radio-labeled DNA, add 1 µl of Label Block to each 10 µl of GLASSMILK® and incubate for 5 minutes at room temperature before adding to the DNA/NaI solution in the next step. 5. Add GLASSMILK® to the DNA/NaI solution as calculated in Step 3. Vortex gently or stir with a pipet tip to ...
Widespread expression of the bovine Agouti gene results from at
... (exons 2, 3 and 4). A single 402 bp fragment was amplified from skin samples of each breed and different tissues (brain, heart, kidney, spleen, lung and liver). PCR fragments were purified and subjected to nucleotide sequence analysis to verify that they contain bovine Agouti coding sequences. These ...
... (exons 2, 3 and 4). A single 402 bp fragment was amplified from skin samples of each breed and different tissues (brain, heart, kidney, spleen, lung and liver). PCR fragments were purified and subjected to nucleotide sequence analysis to verify that they contain bovine Agouti coding sequences. These ...
Identification of a cis-Element That Determines Autonomous DNA
... sequence of the 36 bp may be sufficient to act as a core origin element. This cis-element consensus sequence is an opportunity for focused analyses of core origin elements and the regulation of initiation of DNA replication. ...
... sequence of the 36 bp may be sufficient to act as a core origin element. This cis-element consensus sequence is an opportunity for focused analyses of core origin elements and the regulation of initiation of DNA replication. ...
controversy and its implications Genetic hitchhiking versus
... et al. 2003) and several plant species such as wild tomatoes (Stephan & Langley 1998; Roselius et al. 2005)), it has provoked extensive modelling and analysis efforts. The development of methods for distinguishing BGS and hitchhiking was a major activity in those years (until about 2000). An importa ...
... et al. 2003) and several plant species such as wild tomatoes (Stephan & Langley 1998; Roselius et al. 2005)), it has provoked extensive modelling and analysis efforts. The development of methods for distinguishing BGS and hitchhiking was a major activity in those years (until about 2000). An importa ...
controversy and its implications Genetic hitchhiking versus
... et al. 2003) and several plant species such as wild tomatoes (Stephan & Langley 1998; Roselius et al. 2005)), it has provoked extensive modelling and analysis efforts. The development of methods for distinguishing BGS and hitchhiking was a major activity in those years (until about 2000). An importa ...
... et al. 2003) and several plant species such as wild tomatoes (Stephan & Langley 1998; Roselius et al. 2005)), it has provoked extensive modelling and analysis efforts. The development of methods for distinguishing BGS and hitchhiking was a major activity in those years (until about 2000). An importa ...
Intrinsic sequence specificity of the Cas1 integrase directs
... flap structure (Figure 2). By labelling the single-stranded 5’-flap of the downstream duplex at the 5’- ...
... flap structure (Figure 2). By labelling the single-stranded 5’-flap of the downstream duplex at the 5’- ...
The Chloroplast trnT–trnF Region in the Seed Plant
... gymnosperm lineage Gnetales (Gnetum, Welwitschia, and Ephedra), with especially dense sampling in Gnetum for which we sequenced 41 accessions, representing most of the 25–35 species. The trnL intron has a conserved secondary structure and contains elements that are homologous across land plants, whi ...
... gymnosperm lineage Gnetales (Gnetum, Welwitschia, and Ephedra), with especially dense sampling in Gnetum for which we sequenced 41 accessions, representing most of the 25–35 species. The trnL intron has a conserved secondary structure and contains elements that are homologous across land plants, whi ...
Principles of Genetic Variation
... Genetic variation describes differences between the DNA sequences of individual genomes. Because each of us has two nuclear genomes (a paternal genome and a maternal genome), genetic variation occurs within as well as between individuals. At any genetic locus (DNA region having a unique chromosomal ...
... Genetic variation describes differences between the DNA sequences of individual genomes. Because each of us has two nuclear genomes (a paternal genome and a maternal genome), genetic variation occurs within as well as between individuals. At any genetic locus (DNA region having a unique chromosomal ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.